Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for inducing formation of porcine fat cells by cell signal channel inhibitor

A technology of cell signaling and inhibitors, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve problems such as biological safety impacts, achieve simplicity and safety, prevent gene mutations, and form mechanisms Promoted effect

Inactive Publication Date: 2013-01-23
JILIN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a method for inducing the formation of pig adipocytes by a cell signaling pathway inhibitor, through which the cell signaling pathway inhibitor acts on the cells, and the obtained pig adipocytes have no insertion of foreign genes, which solves the problem of foreign gene insertion caused by transgenic The question of the impact of mutations on biosafety

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inducing formation of porcine fat cells by cell signal channel inhibitor
  • Method for inducing formation of porcine fat cells by cell signal channel inhibitor
  • Method for inducing formation of porcine fat cells by cell signal channel inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 0.5μM Cell signaling pathway inhibitor SB431542 induces transformation of porcine embryonic fibroblasts into adipocytes

[0025] Pig embryonic fibroblasts were divided into 5 × 10 4 piece / cm 2 The proportion of the cells was evenly inoculated into 6cm cell culture plates and cultured with induction medium. The induction medium formula is: 15% (v / v) Knockout Serum Replacement (Invitrogen) serum, 82.8% (v / v) DMEM / F12 (Invitrogen) medium, 0.1mM non-essential amino acids (Invitrogen), 0.055mM β-sulfhydryl Ethanol (Invitrogen), 2 mM L-glutamine (Invitrogen), 0.5 μM SB431542 (Stemgent). 37°C, 5% CO 2 cultured in an incubator.

[0026] The results showed that after 20 days of induction culture, the formation of bright, lipid-rich cells was observed (see figure 1 , figure 2 ). After oil red O staining, round lipid droplets stained red can be observed, and pig fat-specific genes (PPARγ, C / EBPα, β, δ, LPL, AP2, Adipoq, CFD, SLC2A4) were respectively after PCR Specific...

Embodiment 2

[0030] 0.2μM Cell signaling pathway inhibitor SB431542 induces transformation of porcine embryonic fibroblasts into adipocytes

[0031] Pig embryonic fibroblasts were divided into 5 × 10 4 piece / cm 2 The proportion of the cells was evenly inoculated into 6cm cell culture plates and cultured with induction medium. The induction medium formula is: 15% (v / v) Knockout Serum Replacement (Invitrogen) serum, 82.8% (v / v) DMEM / F12 (Invitrogen) medium, 0.1mM non-essential amino acids (Invitrogen), 0.055mM β-sulfhydryl Ethanol (Invitrogen), 2 mM L-glutamine (Invitrogen), 0.2 μM SB431542 (Stemgent). 37°C, 5% CO 2 cultured in an incubator.

[0032] The results showed that after 20 days of induction culture, the formation of bright cells rich in lipid droplets could be observed. After oil red O staining, round lipid droplets stained red can be observed, which proves that 0.2 μM SB431542 can promote the conversion of porcine embryonic fibroblasts into adipocytes, and the calculated ef...

Embodiment 3

[0034] 1μM Cell signaling pathway inhibitor SB431542 induces transformation of porcine embryonic fibroblasts into adipocytes

[0035] Pig embryonic fibroblasts were divided into 5 × 10 4 piece / cm 2The proportion of the cells was evenly inoculated into 6cm cell culture plates and cultured with induction medium. The induction medium formula is: 15% (v / v) Knockout Serum Replacement (Invitrogen) serum, 82.8% (v / v) DMEM / F12 (Invitrogen) medium, 0.1mM non-essential amino acids (Invitrogen), 0.055mM β-sulfhydryl Ethanol (Invitrogen), 2 mM L-glutamine (Invitrogen), 1 μM SB431542 (Stemgent). 37°C, 5% CO 2 cultured in an incubator.

[0036] The results showed that after 20 days of induction culture, the formation of bright cells rich in lipid droplets could be observed. After oil red O staining, round lipid droplets stained red can be observed, which proves that 1 μM SB431542 can promote the transformation of porcine embryonic fibroblasts into adipocytes, and the calculated effic...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for inducing formation of porcine fat cells by a cell signal channel inhibitor. The method comprises the following steps of uniformly inoculating porcine embryo fibroblasts into a Corning cell culture plate according to a ratio of 4-6* 104 cells / cm2; culturing by using an inducing culture medium, and observing the cells, and performing oil red O dyeing to prove that the cells are fat cells after culturing for 20 days in a culture box containing 5% CO2 at 37 DEG C. The cell signal channel inhibitor instead of a transgene is used for inducing cell transformation,so that genic mutation caused by insertion of an exogenous gene into a cell genome can be effectively prevented; by the method, the research on the forming mechanism of the fat cells is facilitated to a certain extent; and the method with convenience and safety can be widely applied and can be used for researching a fat forming mechanism and also can play a roll in disease therapy and other clinical applications.

Description

Technical field [0001] The present invention disclose a method of inducing the formation of pork fat cells to induce the formation of pork fat cells to transform pig cells to transform fat cells, which belongs to the field of cell induction technology. Background technique [0002] Due to the needs of clinical treatment, people need special differentiation cells to treat diseases, so stem cell induction and differentiation techniques are gradually mature, but then they find that some cells can directly differentiate into another type of differential cells without the status of stem cells and report it.The methods are achieved by genetically modified. The cells that differentiate the ending of the ending are directly converted into other types of functional cells: such as the embryo into fibroblasts, cartilage cells, and retinal epithelial cells into muscle cells with shrinkable characteristics;Transform illegal lymphocytes into macrophages; transform the islet -exogenal cells int...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077
Inventor 欧阳红生逄大欣朱建国周杨高飞唐成程全龙泉张明军
Owner JILIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com