A kind of photoreceptor and its synthesis method

A photoreceptor and synthesis method technology, which is applied in chemical instruments and methods, luminescent materials, fluorescence/phosphorescence, etc., can solve the problems of large cytotoxicity and adverse reactions, narrow excitation spectrum, poor photostability, etc., and achieves easy molecular design, Simple synthesis and the effect of preventing gene mutation

Active Publication Date: 2016-09-07
ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as traditional fluorescent materials are concerned, due to the disadvantages of large cytotoxicity and adverse reactions, narrow excitation spectrum, poor photostability, and weak fluorescence intensity, their applications are greatly restricted.

Method used

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  • A kind of photoreceptor and its synthesis method
  • A kind of photoreceptor and its synthesis method
  • A kind of photoreceptor and its synthesis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 (Synthesis of a new photoreceptor):

[0019] Such as figure 1 As shown, the optimized structures of the photosensitive flavoprotein chromophore molecules used in the examples are respectively coded S-pCT (neutral molecule) and S-pCT - (Ionic molecule) means that the optimized structure of the chromophore after replacing S atom with selenium atom Se is coded Se-pCT (neutral molecule) and Se-pCT respectively - (Ionic molecule) said.

[0020] Synthesis of sodium phenylselenide: Add 2.40g (0.01mol) magnesium powder to a 250ml three-necked flask, add 35ml anhydrous ether, and start stirring; add 10ml anhydrous ether and 10.7ml bromobenzene mixture to the dropping funnel, The mixed solution in the dropping funnel was slowly added dropwise to the three-necked flask. After the addition was completed, the temperature was slowly raised to 45°C, and the reaction was refluxed for 45 minutes. Stop heating and cool to 36°C. Add 7.01 g of selenium powder to the three-necked flas...

Embodiment 2

[0022] Example 2 (Measurement of absorption spectrum after selenization reaction)

[0023] Weigh a few milligrams to more than ten milligrams of the target product in a small sample tube and add DMSO to make 10 -3 Standard storage solution of M, stored in the refrigerator at -37℃. When using, use H 2 Dilute the O / DMSO buffer to the required concentration and control the DMSO content at 0.5%.

[0024] The absorption spectra of the photosensitive flavoprotein chromophore molecule S-pCT and the target product Se-pCT after selenization were measured in DMSO solution. Take 3μL of stock solution and 3mL of H 2 O / DMSO buffer solution (0.05M Tris-HCl, 50% DMSO, pH=7.5) is prepared into a 5μM test solution, and the test solution is added to a 1cmⅹ1cmⅹ4cm cuvette, and the absorption spectrum and selenium of the sample can be measured separately The change of spectrum after chemical reaction.

[0025] The experimental results of selenization reaction on broadening the red shift of the spectru...

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Abstract

The invention relates to a novel photoreceptor and a synthetic method thereof, and relates to a novel photoreceptor for generating red shift on a fluorescent chromophore spectrum inside an organism by utilizing selenium reaction. The invention provides a novel photoreceptor for widening the red shift of a light-sensitive xanthoprotein chromophore spectrum and a synthetic method thereof. The novel photoreceptor provided by the invention is synthesized by substituting a selenium atom for a sulfur atom on a light-sensitive xanthoprotein chromophore matrix and utilizing the difference of electronegativity of the sulfur atom and the selenium atom, thereby influencing the optical excitation characteristic of the novel photoreceptor in the organism and realizing the research of photochemical reaction inside an organism cell.

Description

Technical field [0001] The present invention provides a novel photoreceptor and its synthesis method which can be used to broaden the red shift of the light-sensitive flavoprotein chromophore spectrum. The present invention replaces sulfur atoms with selenium atoms on the matrix of the photosensitive flavoprotein chromophore to carry out selenization reaction. Selenium atoms are larger than sulfur atoms and have smaller electronegativity, thereby affecting the light excitation characteristics of photoreceptors in organisms. Realize the research of the photochemical reaction characteristics in the cells of organisms. Background technique [0002] Photoactive Yellow Pretein (PYP) is an important bacterial blue light receptor. Its chromophore is a coumaric acid linked to cysteine ​​via thioimidate, which is located in a multifunctional fusion protein. The signal area regulates the gene expression of chalcone synthase and controls the transmission of blue light signals. Under light...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C391/02C09K11/06G01N21/64
Inventor 赵广久张晓玉李鹏曲宗金
Owner ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
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