Application of Ampelopsis grossedentata total flavone in preparing medicament for preventing and treating adverse reaction of tumor chemoradiotherapy
A technology for the treatment of grapes with dwarf teeth and adverse reactions, which can be applied to anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., and can solve problems such as damage to structural stability, damage to macromolecular functions, and low ability to repair toxic effects.
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Embodiment 1
[0017] Embodiment 1: Extraction and separation preparation of THT
[0018] Extraction process: add purified water to the mixture of dried young leaves and buds of the vine plant, at a weight ratio of 1:8 to 10 (raw material: water), heat to 70°C and soak for 0.5-1 hour , heated to boiling, heat-preserved and extracted for 30-60 minutes, while hot, tangential flow ultrafiltration with ceramic membrane (30KD interception) was used to collect the extract; Purified water, heated to boiling, heat-preserved and extracted for 20-40 minutes, tangential flow ultrafiltration with ceramic membrane (30KD cut-off) while hot, combined and collected extracts; concentrated extracts under reduced pressure to 20%-40% of the original volume % (or the relative density is 1.06-1.10), add edible alcohol until the final concentration of ethanol is 50%-75%, heat to boiling and reflux for 20-60 minutes, press filter or centrifuge to remove precipitation, recover ethanol, and cool the filtrate Stand s...
Embodiment 2
[0019] Embodiment 2: the colorimetric method determination of the flavonoid content of THT
[0020] 1. Principle: The phenolic hydroxyl group in flavonoids can interact with Al 3+ The ions form a yellow complex with a characteristic absorption wavelength of 314nm. There is a linear relationship between color concentration and flavonoid content within a certain range.
[0021] 2. Instruments and Reagents
[0022] Analytical balance, sensitivity 0.0001g; UV-visible spectrophotometer;
[0023] Anhydrous methanol (AR); 5% AlCl3 solution; dihydromyricetin (control standard).
[0024] 3. Detection method
[0025] (1) Preparation of the standard solution: put the standard dihydromyricetin in a weighing dish, dry it at 105°C to constant weight, take it out and let it cool naturally in a desiccator, weigh 0.0504g accurately immediately, dissolve it in methanol, and constant volume to 50ml.
[0026] (2) Preparation of standard concentration gradient sample solution: draw the above...
Embodiment 3
[0030] Example 3: The inhibitory effect of THT on the formation of methemoglobin caused by tertiary peroxide (TBHP)
[0031] 1. Experimental method
[0032] Add packed erythrocytes to double distilled water to make 1% erythrocytes completely hemolyzed, add each sample component and incubate at 37°C for 30min, add TBHP to make the final concentration reach 250uM, continue to incubate for 30min, measure OD 630 Relative changes in methemoglobin content were detected. Calculation of high ferritin production inhibition rate:
[0033] Generation inhibition rate=(OD of oxidative damage group 630 - Sample group OD 630 ) / (Oxidative damage group OD 630 - control group OD 630 )
[0034] 2. Experimental results
[0035] TBHP releases H gradually in the aqueous phase 2 o 2 , H 2 o 2 Catalyzed by free or bound iron in heme to produce superoxide anion and hydroxyl radicals through the Fenton reaction. The maximum light absorption values of the α and β peptide chains of normal ery...
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