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Rapid propagation method for directly inducing buds by using jatropha curcas L. seed leaf regenerated roots

A technology for regenerating roots and Jatropha curcas, which is applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of long transformation cycle, long detection cycle, poor genetic stability of transformed plants, etc., and achieve short cultivation cycle, The effect of fast speed and time-saving multiplication

Inactive Publication Date: 2015-05-20
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, the establishment of the genetic transformation system of Jatropha curcas through Agrobacterium is immature, and the transgenic technology using leaves as explants either needs to go through the callus process, or needs to induce budding before testing, leading to leprosy Tree varieties obtained through the Agrobacterium-mediated genetic transformation system still have problems such as poor genetic stability of transformed plants, long transformation cycle, and long detection cycle.

Method used

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  • Rapid propagation method for directly inducing buds by using jatropha curcas L. seed leaf regenerated roots
  • Rapid propagation method for directly inducing buds by using jatropha curcas L. seed leaf regenerated roots
  • Rapid propagation method for directly inducing buds by using jatropha curcas L. seed leaf regenerated roots

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Ripe shelled Jatropha seeds (see figure 1 ) soaked in 75% alcohol by volume for 10 minutes to kill the bacteria attached to the seed shells, then peeled off the shells under aseptic conditions and sterilized with 75% alcohol by volume for 30 seconds, with a mass concentration of 0.1%. The mercuric chloride aqueous solution was sterilized for 6 min, and then the seeds were taken out from the mercuric chloride aqueous solution and washed 5 times with sterile water to remove residual mercuric chloride, and finally the seeds were soaked in sterile water for 6 h to make the cotyledons of the seeds imbibition. Peel off the cotyledons of the sterilized seeds with a knife and cut off a quarter of the cotyledons below, discard, and use the rest for inoculation.

[0036] The steps and conditions of the rapid propagation of Jatropha curcas that present embodiment provides are as follows:

[0037] (1) Put about 80ml of the first rooting medium into each 120mm diameter petri dish, ...

Embodiment 2

[0048] The mature unshelled Jatropha curcas seeds collected from Laji Village, Renhe District, Panzhihua City, Sichuan Province were sterilized, and the operation method was the same as in Example 1. Peel off the cotyledons of the sterilized seeds with a knife and cut off a quarter of the cotyledons below, discard, and use the rest for inoculation.

[0049] The steps and conditions of the rapid propagation of Jatropha curcas that present embodiment provides are as follows:

[0050] (1) Use a petri dish with a diameter of 120mm, fill each petri dish with about 80ml of the first rooting medium, and inoculate 20-25 pieces of sterilized posterior cotyledons, culture in the dark for 5 days, and then inoculate in the second rooting culture basal, cultivated for 20 days under light intensity of 2000~2500Lx and light time of 16h / d to induce cotyledon rooting;

[0051] (2) Inoculate the roots of a few leaves of Scutellaria edulis on the bud differentiation medium, and culture them for...

Embodiment 3

[0061] In this example, mature Jatropha curcas seeds were collected from Laji Village, Renhe District, Panzhihua City, Sichuan Province. The sterilization operation of Jatropha curcas seeds is identical with embodiment 1. Peel off the cotyledons of the sterilized seeds with a knife and cut off a quarter of the cotyledons below, discard, and use the rest for inoculation.

[0062] The steps and conditions of the rapid propagation of Jatropha curcas that present embodiment provides are as follows:

[0063] (1) Use a petri dish with a diameter of 120mm, fill each petri dish with about 80ml of the first rooting medium, and inoculate 20-25 pieces of sterilized posterior cotyledons, culture in the dark for 4 days, and then inoculate in the second rooting culture basal, cultivated for 18 days under light intensity of 2000~2500Lx and light time of 16h / d to induce cotyledon rooting;

[0064] (2) Inoculate the roots of a few leaves of Scutellaria edulis on the bud differentiation mediu...

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Abstract

The invention provides a rapid propagation method for directly inducing buds by using jatropha curcas L. seed leaf regenerated roots. The method comprises the following steps and conditions: (1) inoculating seed leaves of sterilized jatropha curcas L. seeds in a first rooting culture medium, culturing for 4 to 5 days away from light, then inoculating in a second rooting culture medium for 10 to 24 days, and inducing the seed leaves to root; (2) inoculating roots of the seed leaves in a bud differentiation culture medium to culture for 25 to 30 days, and inducing cluster buds; (3) incising the cluster buds, inoculating in a bud multiplication culture medium to culture for 25 to 30 days, and enabling the cluster buds to multiply; (4) incising buds from the multiplied cluster buds, inoculating in a third rooting culture medium to culture for 4 to 7days, and then transferring to a fourth rooting culture medium to culture, wherein the culturing time in the fourth rooting culture medium takes the limit of achieving the regeneration seedling transplant requirement. The method is short in culturing period and efficient, can maintain the stability of jatropha curcas L. heredity, and is simple and convenient to operate and low in cost.

Description

technical field [0001] The invention belongs to the technical field of rapid plant propagation, and in particular relates to a rapid propagation method using the regenerated root of Jatropha curcas cotyledon and directly inducing buds from the regenerated root. Background technique [0002] Jatropha curcas L., a plant of the genus Davidia of the Euphorbiaceae (Davidiaceae), is distributed in tropical and subtropical regions, with a wide distribution area and rich resources. It can not only obtain biodiesel and biomedical materials from it , active ingredients of pesticides, animal feed, etc., because of its good salt tolerance, low requirements on soil, and adaptability to arid and semi-arid climates, it is a good tree species for afforestation in barren hills in hot and dry valleys, with broad Development and utilization prospects. [0003] However, due to the low yield of Jatropha curcas seeds and the shortage of good varieties, the economic benefits of its propagation ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 徐莺彭天祥陈放
Owner SICHUAN UNIV
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