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Method by utilizing acid electroproduction functional water to inhibit tobacco blackleg germs
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A technology of Tobacco black shank bacteria and electro-generated functional water, which is applied in the field of crop pest control, can solve the problems of small application scale, long period, long half-life and the like, and achieves the effects of simple production method, low production cost and remarkable effect.
Inactive Publication Date: 2013-02-20
SHANDONG AGRICULTURAL UNIVERSITY
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But the above-mentioned method of suppressing black shank has certain limitations, as general investment is big, and cycle is long, and application scale is small, is difficult to apply; And medicament control method can cause certain residue, half-life is long, pollutes environment, therefore also exists certain Shortcomings
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Embodiment 1
[0039] Example 1 Inhibition test of acidic electro-generated functional water on tobacco black shank bacteria
[0043] Tobacco blackleg bacteria were first inoculated on the potato dextrose agar plate prepared in advance, placed in a constant temperature incubator at 28±1 °C, and cultivated for 48 h for later use.
[0044] (3) Treatment of the test
[0045] Dilute the prepared acidic electrogenerated functional water or add sodiumbicarbonate solution to prepare acidic electrogenerated functional water with the following indicators:
[0046]
[0047] Sterile water (control) pH=7.33±0.10, ORP=174 mV, ACC=0 mg / L.
[0048] (4) Test method
[0049] Divide the prepared 42 bacteria-free plates inoculated with tobacco black shank bacteria into 7 groups randomly, use a sketch pen to draw the size of the colonies at this time on the back of each plate, and put them all into the...
Embodiment 2
[0053] Example 2 Effect of contact time between acidic electro-generated functional water and tobacco black shank bacteria on the inhibition rate of tobacco black shank bacteria
[0059] According to the results obtained in Example 1, the slightly acidic electrogenerated functional water was used to test, and the prepared strongly acidic electrogenerated functional water was added with sodiumbicarbonate solution to prepare the slightly acidic electrogenerated functional water with the following indicators:
[0061] Sterile water (control) pH=7.33±0.10, ORP=174 mV, ACC=0 mg / L.
[0062] (4) Test method
[0063] Divide the prepared 30 sterile flat plates inocu...
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Abstract
The invention relates to a method by utilizing acid electroproduction functional water to inhibit tobacco blackleg germs, which is characterized in that: acid electroproduction functional water is adequately contacted with tobacco blackleg germs, the contact time is 30s to 300s, the acid electroproduction functional water is sprayed one time every 24h to 72h, and the inhibition rate of the acid electroproduction functional water to the tobacco blackleg germs is calculated by adopting a computer imageprocessing method; and the subacid electroproduction functional water has an inhibition effect on the tobacco blackleg terms, and the inhibition rate can reach more than 50 percent. The production method is simple, the production cost is low, the processing cost is low, and the acid electroproduction functional water utilized by the method is safe, harmless and residue-free and is free from safety potential danager.
Description
technical field [0001] The invention relates to a method for inhibiting tobacco black shank bacteria with acidic electro-generated functional water, and belongs to the technical field of cropdisease and insectpest control. Background technique [0002] "Electrolyzed Functional Water (EFW)" is also known as electrolyzed ionized water, oxidation potential water and electrolyzed water. It is a general term for the acidic electrogenic functional water and alkaline electrogenic functional water obtained by the dilute solution of chlorine-containing compounds and drinking water or hydrochloric acid and drinking water under the treatment of a special electrolysis device. Among them, the acidic electrogenerated functional water has a lower pH value, a higher oxidation-reduction potential (Oxidation-Reduction Potential, ORP) and a certain available chlorine concentration (Available Chlorine Concentration, ACC). It mainly includes: strong acidic electrogenic functional water (pH &l...
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