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Etiolated two-line sterile line breeding method and use thereof

A technique of sterile lines and etiolation, applied in the field of agriculture, can solve problems such as infeasibility, impracticality, and inoperability, and achieve the effect of improving quality and ensuring the purity of seeds

Active Publication Date: 2012-04-11
江西现代种业股份有限公司
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  • Description
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  • Application Information

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Problems solved by technology

But actual production proves that this method is impractical and infeasible, because the albino phenomenon of the albino-turned-green rice at the seedling stage is obvious and thorough only at a lower temperature (below 22 degrees Celsius), and although it appears at a high temperature, the duration is extremely short ( Generally, it will turn to normal green when it has three leaves), and it is not operable at all

Method used

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  • Etiolated two-line sterile line breeding method and use thereof
  • Etiolated two-line sterile line breeding method and use thereof

Examples

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Effect test

Embodiment 1

[0028] Example 1 Physical mutagenesis Two-line hybrid rice Guangzhan 63S selection and breeding of the yellowing type Guangzhan 63S in the whole growth period, that is, Huangguang S:

[0029] (1) Take the dry seeds of Guangzhan 63S with a water content of 12%, and treat them with cobalt-60 (Co60) gamma radiation at a dose of 300Gy. Live M 0 Seeds, mixed harvest M 1 The seeds of the plant. Mixed planting M 2 When the leaves grow to two leaves and one center, the mutant strains that can be visually identified with yellowing leaves are screened.

[0030] (2) all yellow flower seedlings of step (1) are transplanted to a 1m at the three-leaf stage 3 In the left and right sealed glass covers; with the same leaf age of corn (C 4 crop) as a control, treated at a temperature of 35-40°C for 5 days, and after 80% of the etiolated seedlings withered and died, the undead etiolated seedlings were transplanted into the field, and the plants that kept the yellowing leaves during the grow...

Embodiment 2

[0033] Example 2 application

[0034] In the laboratory, F. 1 generation seed, F 1The seedlings of the generation are all green. If the seeds of Huang Guang S are mixed in consciously, when the seedlings are cultivated to the 4-leaf stage, the impurities can be removed manually, and the etiolated seedlings can be removed 100%. It indicated that the leaf color of Huangguang S was obviously yellowed.

[0035] In Huangguang S seed production, all the female parents are yellow. If there are green or other colored plants, they are hybrid plants, which can be easily removed manually to ensure purity.

[0036] F produced by Huangguang S and other restorer lines with green leaf color 1 Generation seeds, after planting in the field, F 1 The color of the substitute leaves is green, and the yellowed seedlings are hybrid plants, which can be removed conveniently.

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Abstract

The invention provides an etiolated two-line sterile line breeding method, which comprises the following steps: screening etiolated seedlings by using a two-line sterile line as a starting material and by irradiation-induced mutation of seeds; screening etiolated seedlings with high light efficiency by using a high light efficiency screening technique; and selecting a two-line sterile line which have the same properties, except leave color property, with the original two-line sterile line through pedigree selection. When the method is used, the purity of the two-line hybrid rice can be quickly, conveniently and visually identified in the full-growth period in rice production; meanwhile, retaining other properties of the two-line sterile line, the etiolated sterile line can be used in place of a green-leave two-line sterile line.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a breeding method for etiolated two-line male sterile lines and its application. Background technique [0002] Rice occupies an extremely important position in China's grain. With the continuous acceleration of the seed industrialization process, more and more attention has been paid to the purity of seeds, and the production of hybrids has also received more and more attention. There are many reasons for the generation of arils, which can be roughly divided into three categories, mechanical hybridization, biological hybridization, and hybridization caused by self-fertilization of sterile lines. How to solve the mixed seed hybridization and identify false hybrids at the fastest speed has become the most concerned issue for seed producers and farmers. The commonly used identification methods include Hainan or local field planting identification, isozyme electrophoresis ident...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G16/00A01C1/00A01H1/06A01G22/22
Inventor 徐小红李土明周卫营曲姗姗陈龙蔡华镇彭炳生
Owner 江西现代种业股份有限公司
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