Induction method of rosa chinensis receptacle callus tissues

A callus and torus technology, applied in the field of callus induction of rose torus, can solve the problems of low induction rate, difficult callus induction, poor callus quality, etc.

Inactive Publication Date: 2012-04-11
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high content of peroxidase in rose, there are problems such as difficult callus induction, low induction rate, and poor quality of callus formed, which leads to slow progress in the research on callus induction and regeneration of rose
Up to now, some progress has been made in the induction of callus with explants of leaves, petioles, and stems of rose, but there is no report on the in vitro induction of callus of rose receptacle. Implantation, improving the induction rate and quality of rose callus will greatly promote the development of rose industry and bring huge economic benefits

Method used

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  • Induction method of rosa chinensis receptacle callus tissues
  • Induction method of rosa chinensis receptacle callus tissues
  • Induction method of rosa chinensis receptacle callus tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The selection and disinfection of embodiment 1 receptacle explant

[0018] From April to October, select Chinese rose buds with a diameter of 0.4-0.6 cm in the field that are ready to bud, pretreat them in a refrigerator at 4°C for 1-2 days, rinse them with running water for 1-2 hours, and place the buds in 70% ethanol for 30 seconds on a super-clean workbench , then transferred to 0.1% HgCl 2 In the solution for 8 minutes, rinse with sterile water 3 to 4 times, peel off the calyx, petals, stamen group and pistil group, remove the receptacle and cut into 4 pieces for later use.

Embodiment 2

[0019] Example 2: Effects of different concentrations of 2,4-D and 6-BA combinations on callus induction in starter culture

[0020] The aseptic receptacles of rose were inoculated on the MS starting medium containing different concentrations of 6-BA (0, 0.3, 0.5mg / L) and 2,4-D (4.5, 5, 5.5mg / L) combinations. Culture in dark for 7 days, and then switch to low light (800-1000Lx) for culture. After 30 days, the effects of exogenous hormones on callus induction were compared. The results are shown in Table 1: There are significant differences in the induction effects of each starting medium, among which the induction rate of medium MS+2, 4-D 5.0mg / L+6-BA 0.5mg / L is the highest, and the induction quality is the highest. Well, the callus is yellow-green, loosely granular (eg figure 1 shown), and gradually increased with the prolongation of culture time, and can be cultured to 50 days. Therefore, the most suitable starting medium for rose callus induction is MS+2, 4-D 5.0 ​​mg / L+...

Embodiment 3

[0023] Example 3: Effects of Different Concentrations of 2,4-D and 6-BA Combination on Callus Proliferation

[0024] The yellow-green, loose granular initial callus induced on the optimal starting medium (MS+2, 4-D 5.0mg / L+6-BA 0.5mg / L) was transferred to the proliferation medium (MS +2,4-D 2.0~3.0mg / L+6-BA 0.1~0.3mg / L+NAA 1.5~2.5mg / L) for proliferation culture, the callus can proliferate rapidly, among which the proliferation medium MS+ 2,4-D 2.0mg / L+6-BA 0.2mg / L+NAA 2.0mg / L had the best proliferation effect (Table 2).

[0025] Table 2 Effects of different concentrations of 2, 4-D and 6-BA on callus proliferation

[0026]

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Abstract

The invention relates to an induction method of rosa chinensis receptacle callus tissues. The method comprises the following steps: selecting a receptacle explant and carrying out pretreatment and routine sterilization on the receptacle explant; carrying out induction culture on the sterile receptacle in a start culture medium to generate initial callus tissues, wherein the sterile receptacle is firstly cultured in the dark for one week and then cultured in dim lights for 30-50 days; and transferring the initial callus tissues into an enrichment culture medium and carrying out enrichment culture on the initial callus tissues. By utilizing the method provided by the invention, a great quantity of receptacle callus tissues can be quickly acquired and the inductivity is up to 100%, thus the technical problems of low rosa chinensis callus tissue inductivity and poor rosa chinensis callus tissue induction quality can be overcome, and a favorable foundation is laid for rosa chinensis variety improvement and breeding based on a biotechnology.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for inducing callus of rose receptacle. Background technique [0002] Chinese rose (Rosa hybrida L.) is one of the most important garden plants in my country. As an important material for landscaping and greening, rose also occupies an important position in the production and application of fresh cut flowers. On some important ornamental plants such as orchids, tissue culture technology has begun to be applied and commercialized on a large scale. At the same time, new transgenic strains have been bred by using transgenic technology to introduce target genes into crops such as cotton. New rose strains with strong reversibility and specific flower colors provide the possibility. Since the 1990s, the research on tissue culture of rose has been vigorously carried out at home and abroad, but no fundamental breakthrough has been achieved so far. Stu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘会超贾文庆郭丽娟尤扬刘磊齐安国杜晓华王少平徐小博
Owner HENAN INST OF SCI & TECH
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