Preparation method of collagen sponge

Abstract

The invention discloses a preparation method of collagen sponge. The method comprises the following steps: carrying out enzymolysis on cattle heel tendon in protease aqueous solution at pH value of 1.0-5.0; centrifuging the enzymolysis liquid, collecting supernatant, and salting out collagen with saturated salt solution; dialyzing salted-out collagen; cross-linking the dialyzed collagen in a cross-linking agent aqueous solution; and freeze-drying cross-linked collagen gel, cross-linking the collagen in a cross-linking agent aqueous solution and freeze-drying to obtain the collagen sponge. The collagen sponge obtained by the preparation method of the collagen sponge disclosed by the invention has certain elastic and tensile toughness after being immersed in water, and has good biocompatibility and tissue repairing performance, and high protein content and is controllable in degradation.

Description

technical field

[0001] The invention relates to a preparation method of a collagen sponge. Background technique

[0002] Collagen is a kind of extracellular protein, which is a fibrous protein with three peptide chains twisted into a spiral shape. Collagen is the most abundant protein in the human body, accounting for more than 30% of the total protein in the body. Collagen is rich in amino acids such as glycine, proline, and hydroxyproline that the human body needs. Collagen is the most important component of the extracellular matrix. Collagen is considered to be one of the most useful biomaterials due to its superior biocompatibility and safety. The main applications of collagen as a drug delivery system are: collagen masks in ophthalmic surgery, collagen sponges in wounds and burns, pellets and tablets for protein delivery, controlled release gel formulations, controlled release materials for transdermal drug delivery, gene delivery Nanoparticles and cell culture subst...