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Preparation method for artificial seeds of dendrobium candidum

A technology of artificial seeds and dendrobium candidum, applied in gardening methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of artificial seed germination rate, seedling rate and storage conditions, and achieve convenient storage and transportation , the effect of increasing the germination rate

Inactive Publication Date: 2012-05-02
澄思源生物科技(上海)有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern research has made a lot of explorations in artificial endosperm, embedding methods, artificial seed coats, storage methods, etc., but there are still many unresolved issues regarding the germination rate, seedling rate and storage conditions of artificial seeds.

Method used

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Embodiment Construction

[0019] The technical characteristics of the present invention will be further elaborated below in conjunction with specific embodiments.

[0020] (1) Induction of protocorms

[0021] Select healthy plants of Dendrobium officinale that grow vigorously and are disease-free, put each 2 sections into a 500ml beaker, add a small amount of washing powder, tie the mouth of the cup with a single layer of gauze, then tie it tightly with engineering thread, and put it under the tap Rinse for 30 minutes, and the amount of flushing water should be adjusted to make the stem section of Dendrobium candidum float in the water. The main function is to wash away the surface dust and large particle colonies, hyphae, etc. Bring the above-mentioned completed materials to the ultra-clean workbench in the sterile room for secondary disinfection and sterilization: remove the bract leaves of the axillary buds of the stem segment, soak them in 70% alcohol for 25 seconds, and then use 0.1% mercuric chl...

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Abstract

The invention discloses a preparation method for artificial seeds of dendrobium candidum. The method comprises: inducing dendrobium candidum stems in a culture medium so as to obtain protocorms, which are then subjected to proliferation culture, and transferring the protocorms into an MS culture solution with 0.5mg / L of ABA (abscisic acid) for shaking culture, changing the culture solution every 10d, after 30d, selecting a protocorm as an embedding propagule, making artificial seeds by an instillation method, taking the MS culture medium, 4mg / L of 6-BA, 0.4mg / L of NAA, 2% of sodium alginate and 0.2% of carbendazim to prepare basic artificial endosperm, adding 2% of chitosan and 5.0-10% of nanometer TiO2 into sodium alginate so as to form an artificial seed coat matrix, and carrying out research on germination and storage of artificial seeds. The preparation method of the invention can enhance seed germination rate and make the seeds convenient for storage and transportation. Adding of a special material into artificial endosperm or an artificial seed coat in the invention can promote germination of the artificial seeds or prolong storage of the artificial seeds.

Description

technical field [0001] The invention relates to a method for making artificial seeds of Dendrobium officinale, which is a high-efficiency and rapid plant propagation technology, and belongs to the field of biotechnology. Background technique [0002] Dendrobium candidum (Dendrobium candidum), also known as black knot grass, is a precious medicinal plant. It has been regarded as a treasure of nourishing yin and tonifying since ancient times. As early as the Qin and Han Dynasties, "Shen Nong's Materia Medica" stated that "Dendrobium candidum mainly injures, eliminates numbness, lowers qi, nourishes five viscera for weakness, strengthens yin, and takes thick and fat intestines for a long time"; in "Compendium of Materia Medica", Li Shizhen believes that Dendrobium candidum It is "the top grade of this classic, sweet, light, and slightly salty. It is mainly used to treat injuries, eliminate numbness and lower qi, replenish internal deficiency, calm stomach qi, grow muscles, impr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 朱恩灿魏渊源
Owner 澄思源生物科技(上海)有限公司
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