Preparation method for artificial seeds of dendrobium candidum
A technology of artificial seeds and dendrobium candidum, applied in gardening methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of artificial seed germination rate, seedling rate and storage conditions, and achieve convenient storage and transportation , the effect of increasing the germination rate
Inactive Publication Date: 2012-05-02
澄思源生物科技(上海)有限公司
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The invention discloses a preparation method for artificial seeds of dendrobium candidum. The method comprises: inducing dendrobium candidum stems in a culture medium so as to obtain protocorms, which are then subjected to proliferation culture, and transferring the protocorms into an MS culture solution with 0.5mg/L of ABA (abscisic acid) for shaking culture, changing the culture solution every 10d, after 30d, selecting a protocorm as an embedding propagule, making artificial seeds by an instillation method, taking the MS culture medium, 4mg/L of 6-BA, 0.4mg/L of NAA, 2% of sodium alginate and 0.2% of carbendazim to prepare basic artificial endosperm, adding 2% of chitosan and 5.0-10% of nanometer TiO2 into sodium alginate so as to form an artificial seed coat matrix, and carrying out research on germination and storage of artificial seeds. The preparation method of the invention can enhance seed germination rate and make the seeds convenient for storage and transportation. Adding of a special material into artificial endosperm or an artificial seed coat in the invention can promote germination of the artificial seeds or prolong storage of the artificial seeds.
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