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Method for extracting glycosphingolipid total lipids by using nontoxic solvent

A glycosphingolipid and volume-doubling technology, which is applied in the field of preparation of total glycosphingolipids, can solve the problems of complex production process, hindering the promotion and use of glycosphingolipids, and great harm to operators, so as to reduce the operation process and improve the biological quality of the product. Activity, the effect of ensuring safety

Inactive Publication Date: 2012-05-23
吉林省奇健生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It needs multiple extractions with organic solvents and repeated use of different chromatographic methods for purification, the process is complicated, and the production cost is high
Moreover, in the existing method for extracting glycosphingolipids by organic phase distribution, all due to the high toxicity of organic solvents, large usage, large hazards to operators, high energy consumption, serious environmental pollution, complicated production process, long cycle, low yield, Restricting factors such as high cost are difficult to overcome and greatly hinder the promotion and use of glycosphingolipids in industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] 1 kg of fresh pig brain, washed with distilled water, chopped with a knife, added 50 ml of ethyl acetate, and homogenized with a homogenizer to obtain 500 ml of white milk.

[0011] Add 3000 ml of ethyl acetate, stir at 4°C for 12 hours, filter with suction, collect the filtrate, and store at 4°C. Add 3000 ml of ethyl acetate to the residue, stir at 4°C for 12 hours, filter with suction, and collect the filtrate. The 2 filtrates were combined for a total of 6000 ml.

[0012] Rotary evaporation at 50°C, concentrated volume to 250 ml, freeze-dried to obtain 2.1 mg of glycosphingolipid total lipid powder.

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Abstract

The invention discloses a method for extracting glycosphingolipid total lipids by using a nontoxic solvent. According to the method, glycosphingolipid total lipids are extracted from animal brain tissues by using ethyl acetate instead of a toxic organic reagent. No toxic reagent is used in the entire process of the method, so that the safety of a product is ensured.

Description

technical field [0001] The invention belongs to the field of preparation of glycosphingolipids and relates to a method for preparing total glycosphingolipids. Background technique [0002] The parent structure of the glycosphingolipid molecule is ceramide, and the fatty acid is connected to the C-2 amino group of the long-chain sphingosine. Glycosphingolipids are a class of substances with good biological activity, including cerebrosides and gangliosides. Cerebrosides are the most abundant in the brain, followed by the lungs and kidneys, and also in the liver, spleen and serum. Cerebrosides in the brain are mainly galactosides, and their fatty acids are mainly tetracosanoic acid; while glucocerebrosides in the blood are mainly gangliosides, which are a type of acidic glycosphingolipids containing sialic acid. Sialic acid, also known as N-acetylneuraminic acid, is linked to glycolipids through α-glycosidic bonds. Ganglioside molecules are composed of galactose, N-acetylgal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/10
Inventor 周义发席玥台桂花范玉莹孙成新刘洋时嘉宏高娟
Owner 吉林省奇健生物技术有限公司