Application of extract of Antlerpilose grass

An extract, the technology of velvet velvet, applied in the field of application of velvet velvet extract in the preparation of drugs for the prevention and treatment of myocardial hypoxic-ischemic diseases, can solve the problem of the use of velvet velvet and its extract in the prevention and treatment of cardiovascular diseases Reporting and other issues

Active Publication Date: 2012-06-13
SHANGHAI GREEN VALLEY PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant research reports on the prevention and treatment of cardiovascular diseases with velvet antler and its extracts.

Method used

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  • Application of extract of Antlerpilose grass
  • Application of extract of Antlerpilose grass
  • Application of extract of Antlerpilose grass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the preparation of velvet antler extract

[0025] Weigh 10kg of velvet antler medicinal material, chop it up, heat and reflux extraction with 20 times the amount of 50% ethanol for 3 times (8 times, 6 times, 6 times), extract for 1.5 hours each time, filter, combine the filtrates, and concentrate to the equivalent of 1ml In the amount containing 1g of raw medicinal materials (concentration temperature 65-70 ℃). Load AB-8 macroporous resin (column bed volume is about 5L), after loading the sample, use 3 times the amount of distilled water, 15% ethanol to elute, and 60% ethanol to elute, collect the eluted part of 60% ethanol, and evaporate to dryness. velvet antler extract extract 96g.

Embodiment 2

[0026] Embodiment 2, the preparation of velvet grass extract

[0027] Weigh 10kg of pilose antler herb, chop it up, heat and decoct 3 times with 26 times the amount of water (10 times, 8 times, 8 times), decoct for 2 hours each time, filter, combine the filtrates, concentrate to the equivalent of 1mL The amount of 1g of raw medicinal materials (concentration temperature 65-70 ℃); D101 type macroporous resin pretreated on the filtrate (column bed volume is about 5L), after loading, use 6 times the amount of distilled water and 10% ethanol to elute successively, 30 % ethanol for elution, collect the 30% ethanol eluted part, and evaporate to dryness to obtain 110 g of velvet antler extract.

Embodiment 3

[0028] Embodiment 3, DPPH method is measured the scavenging ability of pilose antler extract to free radical

[0029] The velvet antler extract prepared in Example 1 was added with distilled water to make a 1 mg / mL sample solution, and then diluted to 500 μg / mL, 250 μg / mL, 125 μg / mL, 62.5 μg / mL, 31.25 μg / mL and 15.625 μg / mL Six concentrations of mL are available for use. At the same time, 100 μg / mL vitamin E (VE) solution was used as a positive control.

[0030] Take 100 μL of the above-mentioned sample solutions of different concentrations in a 96-well microtiter plate, make three parallel wells for each concentration, then add 100 μL of 1mM DPPH test solution, shake for 30 seconds, and measure the absorbance of each well at 37°C and 517nm wavelength value (Ap). Simultaneously measure the blank absorbance value (Ac) of the sample solution without DPPH and the absorbance value (A max) of the sample solution with DPPH but no sample solution (replacing the sample with 100 μL m...

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PUM

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Abstract

The invention relates to the traditional Chinese medicine field, and discloses an application of a traditional Chinese medicine in preparation of medicines for preventing and treating ischemic cardiomyopathy. According to the invention, a DPPH method is used for determining the Antlerpilose grass extract which possesses removal capability for oxygen free radical, the result shows that the Antlerpilose grass extract is capable of removing the oxygen free radical by a dosage dependence mode. A H2O2 induced myocardial cell damage model is used for detecting the influence of the oxidative damage survival rate and myocardial cell forms of the H2O2 induced mice myocardial cells by the Antlerpilose grass extract. The result shows that the Antlerpilose grass extract is capable of raising the survival rate of the myocardial cells in the dosage dependent mode, obviously improving the form of the myocardial cells and inhibiting the peroxidative damage. In addition, the Antlerpilose grass extract is capable of obviously reducing the content of LDH and MDA in mice myocardial cells. The experiment in vivo shows that the Antlerpilose grass extract can reduce the myocardial infarction of mice caused by coronary artery occlusion. The invention provides the application of the traditional Chinese medicine in preparation of medicines for preventing or treating ischemic cardiomyopathy.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to the application of antler grass extract in the preparation of medicines for preventing and treating myocardial hypoxic-ischemic diseases. Background technique [0002] Antler grass, English name: Antlerpilose grass, is the whole plant of Monochasma savatieri Franch in the family Scrophulariaceae. The stems are thin and hard, branched in clusters. Leaves opposite or three-lobed whorled, dense, internodes shortened; base leaves scale-like, 3-5 mm long; upper leaves narrowly lanceolate, 1-2 cm long, 1-3 mm wide, entire, Apex acuminate, base narrow, sessile. Flowers are solitary in the leaf axils, each flower has 2 linear bracteoles, inserted at the base of the calyx tube; the calyx is tube-shaped, the tube length is 5-7 mm, and the upper part is 4-lobed, the lobes are nearly as long as the calyx tube, 9-9 10; corolla light red, lip-shaped, 2-2.5 cm long. The upper lip...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/80A61P9/10A61P39/06
Inventor 许琼明刘艳丽杨世林师梦凡何文君陈飞龙李笑然
Owner SHANGHAI GREEN VALLEY PHARMA
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