Rotary bioreactor

Abstract

The invention relates to a rotary bioreactor, which comprises a loop chamber with a smooth inner wall, wherein the loop chamber has more than one narrow section; and the inner side wall of a wide section of the loop chamber is provided with an incoming and outgoing pipe through which culturing materials income and outgo. When the rotary bioreactor is in use, a proper amount of culture cells and culture solution are injected into the bioreactor through the incoming and outgoing pipe for culture materials; the bioreactor is driven by a rotation drive device to rotate around the central point as the circle centre on a vertical surface or a plane which forms an acute angle with respect to the vertical surface; under the action of gravity, the culture solution is always located in the chamber on the lower part of the bioreactor below the incoming and outgoing pipe; the culture solution flows through the narrow section and the wide section repeatedly, just like flowing through funnels which are connected end to end as a loop, so that the culture solution can be sufficiently mixed through air exchange, and cultivated non-adherent cells or adherent cells which are adhered to a micro-carrier can grow in a way of suspending in the culture solution. The inner wall of the rotating chamber continuously and repeatedly carries lots of micro-droplets from lower culture solution to be exchanged with upper air, and then the micro-droplets after air exchange are carried into the culture solution, thereby realizing efficient dissolution of oxygen.

Description

technical field [0001] The invention relates to a bioreactor driven by rotary power, which is mainly used for animal cell culture and the production of biotechnology products using animal cells, and can also be used for the cultivation of microorganisms, insect cells, and plant cells and its Production of biotechnological products. Background technique [0002] A bioreactor is a container used to realize a biochemical reaction process, especially to control the culture conditions to produce the best biological products. Bioreactors can be used for in vitro culture of bacteria, spores, fungi, insect cells, poultry cells, mammalian cells, plant cells and other living cells for life science research, cell transplantation treatment of clinical diseases and production of biotechnology products, Including metabolites, antibodies, proteins, viruses, polysaccharides, DNA, cell components, and cell derivatives produced by cultured organisms. Since some biological cells, especially ...

AI Technical Summary

Problems solved by technology

[0005] 3. Air-lift mixing: This type of bioreactor uses the mechanism of blowing air to the culture medium to supply oxygen and simultaneously generating mixing. It is also one of the more commonly used equipment for high-density cultivation of animal cells. The disadvantage is that the bubbles at the gas-liquid interface burst Resulting shear forces can cause cell damage or death, necessitating defoaming

In high-density culture, the mixing is not uniform enough, resulting in high-density dead zone

In addition, the non-disposable use of the device also has cross-contamination issues

[0006] 4. Wave mixing: This type of bioreactor uses waves generated by shaking to achieve mixing and gas exchange at the gas-liquid interface, such as a disposable plastic soft bag reactor (US Patent 6,544,788). It may not be possible to keep the cells in suspension, which may cause precipitation and uneven nutrition and pH of the medium. Especially in small-scale cultures, the cells and microcarriers are more difficult to suspend. They sink to the bottom and rub against each other to separate the cells from the microcarriers. Damage to cells or result in reduced yield of product

[0007] In summary, the existing mixing methods of bioreactors have the problems of high shear force, easy to cause cell damage, and the problems of cell sedimentation, oxygen and nutrient inhomogeneity caused by insufficient mixing.

In addition, most bioreactors also have the problems of complex structure and equipment, high cost and cross-contamination

Images