Method for cultivating aeolosoma hemprichi on large scale
A kind of erythema, a large number of technologies, applied in the direction of animal husbandry, etc., can solve the problems of unsuitable predation, long bacterial culture cycle, easy flocculation, etc., and achieve the effect of simple and feasible production process, not easy to deteriorate, and easy to store
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Embodiment 1
[0018] The cultivation process and steps of C. erythematosus in the present embodiment are as follows:
[0019] (1) Weigh 4.0 grams of rice, add it to 200 ml of distilled water, put the mixture of rice and distilled water into a water bath at 55°C, soak in the water bath for 1 hour, discard the rice precipitated at the bottom, and ultrasonically crush the leaching solution. The power of the cell pulverizer is 600W for 40 minutes, and the crushed solution is settled for 40 minutes, and then the sediment at the bottom is discarded to remove large-sized particles and ensure that the particle size is uniform; after another 24 hours of sedimentation, take the supernatant; The pH value of the above supernatant was adjusted to 6.2; finally, the prepared bait solution was sterilized and stored in the refrigerator for later use.
[0020] (2) Add the abiotic bait solution prepared above into 5 sterile petri dishes prepared in advance, and add 40 μL of the bait solution to each millilit...
Embodiment 2
[0023] The cultivation process and steps of C. erythematosus in the present embodiment are as follows:
[0024] (1) Weigh 6.0 grams of rice, add it to 200 ml of distilled water, put the mixture of rice and distilled water into a water bath at 65°C, soak in the water bath for 2 hours, discard the rice precipitated at the bottom, and ultrasonically crush the leaching solution, then The solution was ultrasonically crushed, and the power of the ultrasonic cell pulverizer was 800W for 20 minutes. After the crushed solution was settled for 40 minutes, the sediment at the bottom was discarded to remove large-size particles and ensure uniform particle size; settle again for 24 Take the supernatant after 1 hour; adjust the pH value of the supernatant to 6.8; finally sterilize the prepared bait solution and store it in the refrigerator for later use.
[0025] (2) Add the abiotic bait solution prepared above into 5 sterile culture dishes prepared in advance, and add 100 μL of the bait s...
Embodiment 3
[0028] The cultivation process and steps of C. erythematosus in the present embodiment are as follows:
[0029] (1) Weigh 5.0 grams of rice, add it to 200 ml of distilled water, put the mixture of rice and distilled water into a water bath at 60°C, soak in the water bath for 1.5 hours, discard the rice precipitated at the bottom, and ultrasonically crush the leaching solution. The power of the cell pulverizer is 800W for 20 minutes, and the crushed solution is settled for 40 minutes, and then the sediment at the bottom is discarded to remove large-sized particles and ensure that the particle size is uniform; after another 24 hours of sedimentation, take the supernatant; The pH value of the above supernatant was adjusted to 6.4; finally the prepared bait solution was sterilized and stored in the refrigerator for later use.
[0030] (2) Add the non-biological bait solution prepared above into 5 sterile culture dishes prepared in advance, and add 80 μL of the bait solution to ea...
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