HPLC (High Performance Liquid Chromatography) analysis method of persimmon leaf extract and preparation of persimmon leaf extract
A technology of persimmon leaf extract and analysis method, which is applied in the field of HPLC analysis of persimmon leaf extract and its preparations, can solve the problems of small amount of information, no good quality stability, etc., and achieve large amount of information and control of persimmon leaf extraction Effects on the quality of substances and their preparations
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Embodiment 1
[0032] 1. Establish a control fingerprint:
[0033] Take persimmon leaf extract 0.03g, accurately weighed, put in a stoppered Erlenmeyer flask, accurately add 10ml of 75% methanol, shake well, and filter through a microporous membrane (0.45μm) to obtain the test solution.
[0034] Precisely draw 10 μl of the test solution for injection, and use the following chromatographic conditions for elution:
[0035] Chromatographic column (TC C of American Agilent Company 18 (4.6mm×250mm, 5μm));
[0036] Acetonitrile-0.2% phosphoric acid solution, binary gradient elution: the elution time is 10min, the concentration changes from 14% to 18% acetonitrile; the elution time is 15min, the concentration is 18% acetonitrile; the elution time is 20min, the concentration From 18% to 30% acetonitrile; elution time is 15min, the concentration changes from 30% to 50% acetonitrile; equilibration time is 20min; flow rate is 1.0ml·min -1 ; Injection volume 10μl; Column temperature 25°C; DAD detecto...
Embodiment 2
[0046] 1. Establish a control fingerprint:
[0047] Take 10 tablets of persimmon leaf extract preparation - Naoxinqing Tablets, remove the coating, crush, weigh 0.50g of the powder, put it in a stoppered Erlenmeyer flask, add 25ml of 65% methanol precisely, weigh it, ultrasonic for 30min, let it cool , Weigh, make up the lost weight with 65% methanol. Microporous membrane (0.45μm) filtration, that is, the test solution.
[0048] Precisely draw 10 μl of the test solution for injection, and use the following chromatographic conditions for elution:
[0049] Chromatographic column (TC C of American Agilent Company 18 (4.6mm×250mm, 5μm));
[0050] Acetonitrile-0.2% phosphoric acid solution, binary gradient elution: the elution time is 11min, the concentration changes from 15% to 17% acetonitrile; the elution time is 16min, the concentration is 17% acetonitrile; the elution time is 21min, the concentration From 17% to 29% acetonitrile; the elution time is 16min, the concentratio...
Embodiment 3
[0062] 1. Create a feature map:
[0063] Take 10 capsules of persimmon leaf extract preparation - Naoxinqing Capsules, remove the capsule shell, crush the contents, weigh 0.50g of the powder, put it in a stoppered Erlenmeyer flask, add 25ml of 85% ethanol precisely, weigh, and ultrasonicate for 15min. Let it cool down, weigh it, and make up the lost weight with 85% ethanol. Microporous membrane (0.35μm) filtration, that is, the test solution.
[0064] Precisely draw 10 μl of the test solution for injection, and use the following chromatographic conditions for elution:
[0065] Chromatographic column (TC C of American Agilent Company 18 (4.6mm×250mm, 5μm));
[0066] Acetonitrile-0.3% phosphoric acid solution, binary gradient elution: the elution time is 9min, the concentration changes from 13% to 19% acetonitrile; the elution time is 14min, the concentration is 19% acetonitrile; the elution time is 19min, the concentration From 19% to 31% acetonitrile; elution time is 16min...
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