Acid-producing bacillus cereus and application thereof

Abstract

The invention discloses a strain called acid-producing bacillus cereus, which is collected in the China center for type culture collection, with the preservation number of CCTCC M 2011352, on October 16th, 2011. The strain can be used for preparing micro-ecological preparations. During application, acid-producing bacillus cereus powder can be used as an individual effective component to be mixed with corn starch to prepare the micro-ecological preparations, or is mixed with at least one other effective component or strain powder and corn starch to prepare composite micro-ecological preparations. The invention further provides a composite micro-ecological preparation M3 which is prepared by three high-temperature-resistant strains including bacillus subtilis N9-1-35, clostridium butyricum Cb-2, the acid-producing bacillus cereus and corn starch according to the mass ratio of 1:2:2:5, wherein the viable counts of the strain powder are larger than or equal to 1.0X109cfu / g, 5.0X108cfu / g and 5.0X108cfu / g respectively.

Description

technical field

[0001] The invention relates to a strain of acidogenic bacillus cereus and its application. Background technique

[0002] Lactic acid bacteria produce organic acids, various digestive enzymes and lactic acid bacteria during the fermentation process. Adding lactic acid bacteria probiotics to livestock and poultry feed can effectively reduce the pH in the gastrointestinal tract, inhibit the growth of pathogenic bacteria, increase feed remuneration, and enhance the immunity of livestock and poultry. In recent years, the research on lactic acid bacteria probiotics has become more and more in-depth. However, generally lactic acid bacteria have high nutritional requirements and poor resistance to adverse environments, especially the ability to resist drying, high temperature, gastric acid and bile. Therefore, the types and quantities of lactic acid bacteria that can enter the intestinal tract alive are few. . Ordinary lactic acid bacteria have poor tolerance to t...

Examples

Embodiment 1

[0039]Example 1 Screening and other studies of acidogenic bacillus

[0040] 1. Screening of acidogenic bacillus

[0041] 1 Test material

[0042] 1.1 Separation source: chicken intestine, chicken manure, pig manure, cow manure

[0043] 1.2 Medium:

[0044] 1.2.1 Culture medium for strain isolation

[0045] Basal medium: yeast extract 0.5%, peptone 0.5%, glucose 0.5%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.02%, manganese sulfate 0.08%, calcium carbonate 1.5%, agar 1.5%.

[0046] 1.2.2 Medium for strain culture

[0047] Seed medium: 0.1% yeast extract, 0.1% peptone, 0.1% glucose, 0.1% potassium dihydrogen phosphate, 0.02% magnesium sulfate, 0.02% manganese sulfate, pH 7.0-7.5.

[0048] Fermentation medium (basic): 0.5% yeast extract, 0.5% peptone, 0.5% glucose, 0.5% dipotassium hydrogen phosphate, 0.02% magnesium sulfate, and 0.08% manganese sulfate.

[0049] 2 strain screening

[0050] 2.1 Isolation and purification method of acidogenic spores: use stra...

Embodiment 2

[0216] Embodiment two industrial large-scale production process of acidogenic bacillus

[0217] The fermentation culture formula of acidogenic bacillus liquid industrial mass production is as follows:

[0218] Seed medium: yeast extract 0.5%, peptone 0.6%, glucose 1.0%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.02%, manganese sulfate 0.08%.

[0219] Liquid fermentation medium: corn flour: 3.0%, soybean flour: 3.2%, bran: 2.5%, ammonium sulfate: 0.8%, magnesium sulfate: 0.01%, disodium hydrogen phosphate: 1%, sodium dihydrogen phosphate: 0.075% , Trisodium lemon: 0.1%.

[0220] The production process of acidogenic spores is as follows:

[0221] (1) First-class seeds: inoculate on a slant, fill 100mL in a 500mL triangular flask, culture at 35°C, 220rpm on a shaker for 14 hours; insert second-class seed solution for expansion.

[0222] (2) Secondary seeds: 1L in a 5L triangular flask, 6% inoculum, 35°C, 220rpm shaker culture for 16 hours; access to the third-gra...

Embodiment 3

[0225] Example 3 Application Test of Composite Probiotics M3

[0226] The compound micro-ecological preparation M3 is often a combination of several strains of probiotics in order to better play the role of probiotics. In order to apply acidogenic bacillus GF1 to production and as a theoretical basis for its use in granular materials, a compound micro-ecological preparation was designed. Ecological preparation M3.

[0227] 1. Compound probiotic preparation M3 formula

[0228] This product consists of three high-temperature-resistant strains of Bacillus subtilis N9-1-35 (5 × 10 8 cfu / g) (preserved in China Center for Type Culture Collection on August 31, 2011, its preservation number is: CCTCC M 2011301), Clostridium butyricum Cb-2 (5×10 8 cfu / g) (preserved in China Center for Type Culture Collection on November 09, 2011, its preservation number is: CCTCC M2011384), Acidogenic Bacillus GF1 (1.0×10 9 cfu / g) and corn starch (commercial name: corn starch; product number: 692042...

technical field

[0001] The invention relates to a strain of acidogenic bacillus cereus and its application. Background technique

[0002] Lactic acid bacteria produce organic acids, various digestive enzymes and lactic acid bacteria during the fermentation process. Adding lactic acid bacteria probiotics to livestock and poultry feed can effectively reduce the pH in the gastrointestinal tract, inhibit the growth of pathogenic bacteria, increase feed remuneration, and enhance the immunity of livestock and poultry. In recent years, the research on lactic acid bacteria probiotics has become more and more in-depth. However, generally lactic acid bacteria have high nutritional requirements and poor resistance to adverse environments, especially the ability to resist drying, high temperature, gastric acid and bile. Therefore, the types and quantities of lactic acid bacteria that can enter the intestinal tract alive are few. . Ordinary lactic acid bacteria have poor tolerance to t...

Examples

Embodiment 1

[0039]Example 1 Screening and other studies of acidogenic bacillus

[0040] 1. Screening of acidogenic bacillus

[0041] 1 Test material

[0042] 1.1 Separation source: chicken intestine, chicken manure, pig manure, cow manure

[0043] 1.2 Medium:

[0044] 1.2.1 Culture medium for strain isolation

[0045] Basal medium: yeast extract 0.5%, peptone 0.5%, glucose 0.5%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.02%, manganese sulfate 0.08%, calcium carbonate 1.5%, agar 1.5%.

[0046] 1.2.2 Medium for strain culture

[0047] Seed medium: 0.1% yeast extract, 0.1% peptone, 0.1% glucose, 0.1% potassium dihydrogen phosphate, 0.02% magnesium sulfate, 0.02% manganese sulfate, pH 7.0-7.5.

[0048] Fermentation medium (basic): 0.5% yeast extract, 0.5% peptone, 0.5% glucose, 0.5% dipotassium hydrogen phosphate, 0.02% magnesium sulfate, and 0.08% manganese sulfate.

[0049] 2 strain screening

[0050] 2.1 Isolation and purification method of acidogenic spores: use stra...

Embodiment 2

[0216] Embodiment two industrial large-scale production process of acidogenic bacillus

[0217] The fermentation culture formula of acidogenic bacillus liquid industrial mass production is as follows:

[0218] Seed medium: yeast extract 0.5%, peptone 0.6%, glucose 1.0%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.02%, manganese sulfate 0.08%.

[0219] Liquid fermentation medium: corn flour: 3.0%, soybean flour: 3.2%, bran: 2.5%, ammonium sulfate: 0.8%, magnesium sulfate: 0.01%, disodium hydrogen phosphate: 1%, sodium dihydrogen phosphate: 0.075% , Trisodium lemon: 0.1%.

[0220] The production process of acidogenic spores is as follows:

[0221] (1) First-class seeds: inoculate on a slant, fill 100mL in a 500mL triangular flask, culture at 35°C, 220rpm on a shaker for 14 hours; insert second-class seed solution for expansion.

[0222] (2) Secondary seeds: 1L in a 5L triangular flask, 6% inoculum, 35°C, 220rpm shaker culture for 16 hours; access to the third-gra...

Embodiment 3

[0225] Example 3 Application Test of Composite Probiotics M3

[0226] The compound micro-ecological preparation M3 is often a combination of several strains of probiotics in order to better play the role of probiotics. In order to apply acidogenic bacillus GF1 to production and as a theoretical basis for its use in granular materials, a compound micro-ecological preparation was designed. Ecological preparation M3.

[0227] 1. Compound probiotic preparation M3 formula

[0228] This product consists of three high-temperature-resistant strains of Bacillus subtilis N9-1-35 (5 × 10 8 cfu / g) (preserved in China Center for Type Culture Collection on August 31, 2011, its preservation number is: CCTCC M 2011301), Clostridium butyricum Cb-2 (5×10 8 cfu / g) (preserved in China Center for Type Culture Collection on November 09, 2011, its preservation number is: CCTCC M2011384), Acidogenic Bacillus GF1 (1.0×10 9 cfu / g) and corn starch (commercial name: corn starch; product number: 692042...

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