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Method for preparing collagen anti-oxidation peptide by using pig skin

A technology of pigskin collagen and collagen, which is applied in the field of preparation of antioxidant peptides, can solve the problems of wide application of restriction enzyme method, lack of protease, and product generation, and achieve the effects of reducing production costs, easy direct absorption, and increasing added value

Inactive Publication Date: 2014-04-02
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, researchers at home and abroad mostly use enzymatic hydrolysis of collagen, and use protease to catalyze the synthesis of active peptides. The most obvious side effect is the hydrolysis of protease itself, which will cause many unnecessary products to be generated. In addition, there is a lack of suitable proteases and yields. Low technical problems also limit the wide application of enzymatic method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Utilize pigskin to prepare the method for collagen antioxidant peptide, described preparation method is:

[0026] Step 1, take 3% pigskin collagen and 97% water with a concentration of 90% by weight, mix evenly to form a pigskin collagen solution, and use a microporosity membrane of 0.45 μm to carry out the pigskin collagen solution Filtration, sterilization, standby;

[0027] Step 2, take 2 parts of Bacillus subtilis bacterial liquid and 1 part of Bacillus licheniformis bacterial liquid according to the ratio of parts by weight, mix evenly to form a mixed bacterial liquid, and set aside;

[0028] Step 3: Inoculate 3% of the mixed bacterial liquid into 97% of the sterilized pigskin collagen solution in Step 1 by weight percentage, and ferment and cultivate for 24 hours at 32°C to form a fermented liquid for subsequent use;

[0029] Step 4, send the fermented liquid obtained in step 3 into a centrifuge, and centrifuge for 30 minutes at a speed of 10,000 rpm, take the ce...

Embodiment 2

[0032] Utilize pigskin to prepare the method for collagen antioxidant peptide, described preparation method is:

[0033] Step 1: Take 4% pigskin collagen and 96% water with a concentration of 95% by weight, mix uniformly to form a pigskin collagen solution, and use a microporosity membrane of 0.45 μm to carry out the pigskin collagen solution Filtration, sterilization, standby;

[0034] Step 2, take 2 parts of Bacillus subtilis bacterial liquid and 1 part of Bacillus licheniformis bacterial liquid according to the ratio of parts by weight, mix evenly to form a mixed bacterial liquid, and set aside;

[0035] Step 3: inoculate 5% of the mixed bacterial solution into 95% of the sterilized pigskin collagen solution in step 1 by weight percentage, and ferment and cultivate for 48 hours at 34°C to form a fermented liquid for subsequent use;

[0036] Step 4, send the fermented liquid obtained in step 3 into a centrifuge, and centrifuge for 30 minutes at a speed of 10,000 rpm, take t...

Embodiment 3

[0039] Utilize pigskin to prepare the method for collagen antioxidant peptide, described preparation method is:

[0040] Step 1, get 6% concentration by weight percentage and be 98% pigskin collagen and 94% water, mix evenly, form pigskin collagen solution, utilize the microporosity membrane of 0.45 μ m to carry out pigskin collagen solution Filtration, sterilization, standby;

[0041] Step 2, take 2 parts of Bacillus subtilis bacterial liquid and 1 part of Bacillus licheniformis bacterial liquid according to the ratio of parts by weight, mix evenly to form a mixed bacterial liquid, and set aside;

[0042] Step 3. Inoculate 6% of the mixed bacterial solution into the 94% pigskin collagen solution sterilized in Step 1 by weight percentage, and ferment and cultivate for 72 hours at 37° C. to form a fermented liquid for subsequent use;

[0043] Step 4, send the fermented liquid obtained in step 3 into a centrifuge, and centrifuge for 30 minutes at a speed of 10,000 rpm, take the...

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Abstract

The invention discloses a method for preparing collagen anti-oxidation peptide by using pig skin. The method comprises the following steps of: inoculating 3 to 6 percent of mixed bacteria solution to 94 to 97 percent of pig skin collagen solution, performing fermentation culture for 24 to 72 hours at the temperature of between 32 and 37 DEG C to form a fermentation solution, centrifuging the fermentation solution for 30 minutes at the rotating speed of 10,000 revolutions per minute, filtering the centrifuged supernate to obtain filtrate, filtering the filtrate through an ultra-filtration membrane with intercepting molecular weight of 1,000 to 5,000, and thus obtaining the collagen anti-oxidation peptide. The method for preparing the collagen anti-oxidation peptide by using the pig skin is simple in process and convenient to operate, the utilization value of the pig skin is improved, and the economic benefits are increased.

Description

technical field [0001] The invention relates to a method for preparing antioxidant peptides, in particular to a method for preparing collagen antioxidant peptides from pigskin. Background technique [0002] my country is a big pig raising country. With the continuous development of science and technology, the pig raising industry has also developed rapidly. According to the data, the number of pigs in stock, the number of slaughtered pigs, and the output of pork in China have ranked first in the world for many years. China's pork output accounts for about half of the world's output, and pork output accounts for 69.4% of my country's total meat output. A large number of by-products are produced while producing pork, and pig skin is one of them. One of the most important applications of animal skin is tanning, but due to the poor texture of pig skin itself, pig skin is rarely used in the leather industry at present. In addition, because pig skin has unacceptable peculiar smell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C12R1/10C12R1/125
Inventor 张慧芸朱文学刘丽莉康怀彬杨芳宁庄军辉
Owner HENAN UNIV OF SCI & TECH