Classification and determination of Salmonella parayphi A, Salmonella parayphi B and Salmonella parayphi C through Fourier transform infrared spectrum technology

Abstract

The invention belongs to classification and determination to three Salmonella parayphis and particularly relates to classification and determination of Salmonella parayphi A (S. parayphi A), Salmonella parayphi B (S. parayphi B) and Salmonella parayphi C (S. parayphi C) by using a Fourier transform infrared spectrum (FT-IR) technology to be combined with calculation in chemometrics (hierarchical cluster analysis). The method comprises the following steps of: establishing FT-IR spectrum information databases for the three Salmonella parayphis; culturing bacteria; manufacturing a zinc selenide (ZnSe) window, collecting and pro-processing spectrums; and finally judging a result by using the hierarchical cluster analysis. The method disclosed by the invention has the advantages of high resolution ratio, fast speed, simplicity in operation and low cost.

Description

technical field [0001] The invention belongs to the classification and identification of three kinds of Salmonella paratyphi, specifically using Fourier transform infrared spectroscopy (FT-IR) technology to identify Salmonella paratyphi A (Salmonella parayphi A, S.parayphi A), Salmonella paratyphi B (Salmonella parayphi B, S.parayphi B), Salmonella paratyphi C (Salmonella parayphi C, S.parayphi C) for rapid classification and identification. Background technique [0002] Salmonella paratyphi type A, type B, and type C are important pathogenic bacteria in water and food, and can cause acute infectious diseases of paratyphoid fever. Paratyphoid is one of the Class B infectious diseases reported in my country’s Law on the Prevention and Control of Infectious Diseases—and it is also a common public health problem faced by the world, especially in developing countries. The rapid and effective classification, identification and detection technology of Salmonella typhi has importan...

AI Technical Summary

Problems solved by technology

However, the application of spectral technology in the classification and identification of microorganisms is not yet systematic and mature, especially for the FT-IR identification method of some bacteria within the scope of classification, it is not yet standardized, and it needs to be unified and form a standard method

At present, there is no standardized and unified FT-IR classification and identification method for Salmonella paratyphi A, B, and C at home and abroad, and there is no disclosure of the FT-IT spectral information sharing database for the three types of Salmonella paratyphi. It can make up for the above defects and establish a standardized and unified FT-IR classification and identification method for the three kinds of Salmonella paratyphi

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