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Method for detecting quality of thinleaf milkwort root-bark based on high performance liquid chromatography fingerprint

A technology of high performance liquid chromatography and fingerprint spectrum, which is applied in the quality inspection field of Yuanzhi, and achieves the effects of good stability, simple method and good reproducibility.

Active Publication Date: 2013-08-14
ECONOMIC CROPS RES INST OF SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, in the production and circulation of traditional Chinese medicine, there is no quality control method that can comprehensively and comprehensively reflect the quality variation of traditional Chinese medicine products, and can effectively control the quality of the whole process

Method used

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  • Method for detecting quality of thinleaf milkwort root-bark based on high performance liquid chromatography fingerprint
  • Method for detecting quality of thinleaf milkwort root-bark based on high performance liquid chromatography fingerprint
  • Method for detecting quality of thinleaf milkwort root-bark based on high performance liquid chromatography fingerprint

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 The establishment of Polygala high performance liquid chromatography fingerprint

[0045] 1.1 Instruments, reagents and materials

[0046] 11.1 Instruments

[0047] 2695 High Performance Liquid Chromatography (U.S. Waters Corporation); 2996 Secondary Array Detector (DAD) (U.S. Waters Corporation); Water Chemical Chromatography Workstation (U.S. Waters Corporation); Soxhlet Extractor; BUG25-12 Ultrasonic Instrument; Fingerprint similarity evaluation system version 2004A.

[0048] 1.1.2 Reagents

[0049] Methanol and acetonitrile are chromatographically pure, phosphoric acid, ether, ethyl acetate, n-butanol, etc. are analytically pure, and water is redistilled.

[0050] 1.1.3 Materials

[0051] There were 23 copies of Polygala medicinal materials from different origins, 5 medicinal materials of different growth stages of Polygala cultivated, and 8 copies of Polygala root heart (Table 2 and Table 3).

[0052] 1.2 Investigation of experimental methods

[...

Embodiment 2

[0149] Example 2 Polygala index component polygala acid content determination

[0150] 1.1 Instruments, reagents and materials

[0151] 1.1.1 Instruments

[0152] Dionex Summit HPLC, Detector UVD 170U, Column Thermostat TCC-100; Zhejiang University N2000 chromatography data workstation, Soxhlet extractor.

[0153] 1.1.2 Reagents

[0154] Methanol (chromatographically pure), phosphoric acid (analytical pure), redistilled water, etc.

[0155] Reference substance: polygalic acid (provided by the Key Laboratory of Standardization of Chinese Medicinal Materials, Ministry of Education, Chengdu University of Traditional Chinese Medicine).

[0156] 1.1.2 Materials

[0157] Samples: 21 parts of polygala medicinal materials (16 parts of wild samples, 5 parts of cultivated samples), 5 parts of root heart, and 11 parts of leaves (see Table 22-Table 24).

[0158] All samples were identified as Polygala tenuifolia Willd and P.sibirical L.

[0159] 1.2 Test method

[0160] 1.2.1 Chrom...

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Abstract

The invention discloses a method for detecting the quality of thinleaf milkwort root-bark based on a high performance liquid chromatography (HPLC) fingerprint for the first time. The method comprises the following steps of: preparing a sample solution, selecting chromatographic conditions, determining, drawing a reference spectrum, and detecting the quality of a sample. Further, a standard HPLC fingerprint of the thinleaf milkwort root-bark is established by taking polygalic acid as a reference substance, and can effectively and accurately characterize the quality of the thinleaf milkwort root-bark. By the method for detecting the quality of the thinleaf milkwort root-bark based on the HPLC fingerprint, the quality of the thinleaf milkwort root-bark can be comprehensively and effectively monitored, and the stability, uniformity and controllability of the quality of the thinleaf milkwort root-bark are ensured. The invention has the advantages that: the method is simple and convenient, and is high in stability, high in precision and high in reproducibility; the advantages and disadvantages of the product can be quickly and accurately identified; and a reliable basis is provided for quality detection of medicines.

Description

technical field [0001] The invention relates to a method for detecting the quality of polygala, in particular to a method for detecting the quality of polygala based on high performance liquid chromatography fingerprints. Background technique [0002] Polygala is a commonly used bulk medicinal material, and its cultivation area is widely distributed. In recent years, hundreds of new drugs, special drugs, and Chinese patent medicines have been developed with Polygala. With the increasing market demand year by year, the production of "safe, high-quality, stable, and controllable" products meets the technical and quality requirements for polygala production at home and abroad. Polygala medicinal materials will bring huge social and economic benefits. [0003] Due to different production areas or different harvesting seasons, there are great differences in the internal quality of polygala. Taking the test results of the index component polygalaic acid as an example, the differe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 田洪岭赵云生王耀琴郭淑红程永钢郝耀鹏魏海英张成荣
Owner ECONOMIC CROPS RES INST OF SHANXI ACAD OF AGRI SCI
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