Detection method for pathogenicity of maize bacterial wilt source fusarium in maize seedling stage

A detection method, Fusarium technology, applied in the field of detection, can solve the problems of complex detection, inaccurate measurement results, and inaccurate results of the Guo-Maier method, and achieve the effect of simple detection method, shortened time, and accurate results

Inactive Publication Date: 2012-07-18
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex soil environment and the fact that the roots are easily damaged when cleaning the roots, it is easy to cause statistical errors and result in inaccurate results. However, the Guo-Mai's method is complicated to detect and the results are likely to be inaccurate at the same time.

Method used

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  • Detection method for pathogenicity of maize bacterial wilt source fusarium in maize seedling stage
  • Detection method for pathogenicity of maize bacterial wilt source fusarium in maize seedling stage
  • Detection method for pathogenicity of maize bacterial wilt source fusarium in maize seedling stage

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preparation example Construction

[0044] Step 1, preparation of water-agar mixed medium

[0045] 1) Add 17g of agar to 1000mL of water, heat to dissolve the agar, then add 1.5g of calcium nitrate, 1.5g of potassium nitrate, 1g of ammonium dihydrogen phosphate and 0.5g of magnesium sulfate, stir well to form a mixed solution, and pack In 300mL Erlenmeyer flasks, each bottle is 100mL, sealed with propylene film and left with a vent hole, and then each Erlenmeyer flask containing the mixed solution is sent into an autoclave, at a pressure of 0.14MPa and a temperature of 121°C, Sterilize for 30 minutes to prepare nutrient agar medium for subsequent use;

[0046] 2) Under sterile conditions, add 100mL of deionized water to each sterilized nutrient agar medium in step 1), and then crush the nutrient agar medium to particles with a particle size of 0.5--0.8cm Stop at the same time, mix evenly to form a water-agar mixed medium, and set aside;

[0047] Step two, corn seedling cultivation

[0048] Take 1 part of corn...

Embodiment 1

[0074] A method for detecting the pathogenicity of corn solanacear pathogen Fusarium to corn seedling stage, the detection method is:

[0075] Step 1, preparation of water-agar mixed medium

[0076] 1) Add 17g of agar to 1000mL of water, heat to dissolve the agar, then add 1.5g of calcium nitrate, 1.5g of potassium nitrate, 1g of ammonium dihydrogen phosphate and 0.5g of magnesium sulfate, stir well to form a mixed solution, and pack In 300mL Erlenmeyer flasks, each bottle is 100mL, sealed with propylene film and left with a vent hole, and then each Erlenmeyer flask containing the mixed solution is sent into an autoclave, at a pressure of 0.14MPa and a temperature of 121°C, Sterilize for 30 minutes to prepare nutrient agar medium for subsequent use;

[0077] 2) Under sterile conditions, add 100mL of deionized water to each sterilized nutrient agar medium in step 1), and then crush the nutrient agar medium until the particle size is 0.5cm. Mix evenly to form a water-agar mixe...

Embodiment 2

[0099] A method for detecting the pathogenicity of corn solanacear pathogen Fusarium to corn seedling stage, the detection method is:

[0100] Step 1, preparation of water-agar mixed medium

[0101]1) Add 17g of agar to 1000mL of water, heat to dissolve the agar, then add 1.5g of calcium nitrate, 1.5g of potassium nitrate, 1g of ammonium dihydrogen phosphate and 0.5g of magnesium sulfate, stir well to form a mixed solution, and pack In 300mL Erlenmeyer flasks, each bottle is 100mL, sealed with propylene film and left with a vent hole, and then each Erlenmeyer flask containing the mixed solution is sent into an autoclave, at a pressure of 0.14MPa and a temperature of 121°C, Sterilize for 30 minutes to prepare nutrient agar medium for subsequent use;

[0102] 2) Under sterile conditions, add 100mL of deionized water to each sterilized nutrient agar medium in step 1), and then crush the nutrient agar medium until the particle size is 0.7cm. Mix evenly to form a water-agar mixed...

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Abstract

The invention relates to a detection method for the pathogenicity of maize bacterial wilt source fusarium in a maize seedling stage. The detection method comprises the following steps of: preparing maize infected by the maize bacterial wilt source fusarium, designing and synthesizing a primer, extracting the DNA (deoxyribonucleic acid) of the maize infected by the maize bacterial wilt source fusarium, amplifying and separating target genes, separating amplified end-products, carrying out sequencing evaluation, comparing in a Genbank, and determining that the maize is infected by the maize bacterial wilt source fusarium. The detection method for the pathogenicity of the maize bacterial wilt source fusarium in the maize seedling stage has the advantages that an identification method is simple in operation, an evaluation result is obtained rapidly and accurate, and the detection method can be applied to the actual production.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method for the pathogenicity of corn bacterial wilt pathogen Fusarium to corn seedling stage. Background technique [0002] Corn is the food crop with the highest total yield in the world. Corn bacterial wilt, also known as corn stalk rot and stalk rot, is one of the major diseases of corn in the world. Its harm is very serious. According to relevant data reports, in some countries, the loss of corn yield due to corn bacterial wilt can reach 25-33%, and some even reach more than 50%. [0003] Corn bacterial wilt is a soil-borne disease. Due to the complex soil environment, when studying soil-borne diseases and isolating their pathogenic bacteria, they are often interfered by various factors, resulting in difficulties in the isolation and identification of pathogenic bacteria. In the vascular bundle disease of the stem, when the disease can be seen with the naked eye, it is oft...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04
Inventor 侯军林晓民张建祥张春奇韩文忠
Owner HENAN UNIV OF SCI & TECH
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