Reverse transcription-polymerase chain reaction (RT-PCR) detection for porcine reproductive and respiratory syndrome virus and classical swine fever virus and special primers for same

Abstract

The invention discloses reverse transcription-polymerase chain reaction (RT-PCR) detection for porcine reproductive and respiratory syndrome virus and classical swine fever virus and special primers for same. The invention provides the special primers for detecting the viruses. The special primers comprise a primer pair A and a primer pair B, wherein the primer pair A comprises a primer 1 and a primer 2; the primer pair B comprises a primer 3 and a primer 4; and nucleotide sequences of the primers 1, 2, 3 and 4 are sequences 1, 2, 3 and 4 in a sequence table. Experiments prove that: by the multiplex RT-PCR detection method for the porcine reproductive and respiratory syndrome virus and the classical swine fever virus, a classical porcine reproductive and respiratory syndrome virus strain, a high pathetic porcine reproductive and respiratory syndrome virus strain and the classical swine fever virus can be simultaneously detected through once reaction, and the method has a good application prospect on clinical diagnosis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to RT-PCR detection of porcine reproductive and respiratory syndrome virus and swine fever virus and special primers thereof. Background technique [0002] The RT-PCR detection method of porcine reproductive and respiratory syndrome virus (PRRSV) and classical swine fever virus (CSFV) is currently a common method for rapid detection of these two pathogens, and is widely used in laboratory pathogen diagnosis. Compared with the virus isolation method, the RT-PCR method is faster and easier to diagnose, and the virus isolation is relatively time-consuming, which is not conducive to rapid clinical diagnosis. At present, the laboratory diagnosis of viral diseases mainly uses the PCR / RT-PCR method. The RT-PCR kits of PRRSV and CSFV have been commercialized and put into clinical application. [0003] The current multi-virus mixed infection of swine disease is quite serious. Clinically, porci...

AI Technical Summary

Problems solved by technology

The commercial PRRSV and CSFV detection RT-PCR kits are all single-item RT-PCR. One kit can only detect one virus in one test. For the same pig disease that may be mixed with PRRSV, HP-PRRSV, and CSFV It is expected that 3 kinds of kits and 3 RT-PCR experiments are required to confirm the diagnosis of the pathogen, which is not only time-consuming and labor-intensive, but also has a high cost of detection, which is neither economical nor fast

Images