Reverse transcription-polymerase chain reaction (RT-PCR) detection for porcine reproductive and respiratory syndrome virus and classical swine fever virus and special primers for same

A technology for RT-PCR and respiratory syndrome, which is applied in the field of RT-PCR detection and its special primers, can solve the problems of high detection cost, time-consuming and laborious, and not fast, and achieve strong sensitivity, good application prospects, and high specificity Effect
CN102586477AInactive Publication Date: 2012-07-18山东省动物疫病预防与控制中心
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
山东省动物疫病预防与控制中心
Publication Date
2012-07-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses reverse transcription-polymerase chain reaction (RT-PCR) detection for porcine reproductive and respiratory syndrome virus and classical swine fever virus and special primers for same. The invention provides the special primers for detecting the viruses. The special primers comprise a primer pair A and a primer pair B, wherein the primer pair A comprises a primer 1 and a primer 2; the primer pair B comprises a primer 3 and a primer 4; and nucleotide sequences of the primers 1, 2, 3 and 4 are sequences 1, 2, 3 and 4 in a sequence table. Experiments prove that: by the multiplex RT-PCR detection method for the porcine reproductive and respiratory syndrome virus and the classical swine fever virus, a classical porcine reproductive and respiratory syndrome virus strain, a high pathetic porcine reproductive and respiratory syndrome virus strain and the classical swine fever virus can be simultaneously detected through once reaction, and the method has a good application prospect on clinical diagnosis.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to RT-PCR detection of porcine reproductive and respiratory syndrome virus and swine fever virus and special primers thereof. Background technique

[0002] The RT-PCR detection method of porcine reproductive and respiratory syndrome virus (PRRSV) and classical swine fever virus (CSFV) is currently a common method for rapid detection of these two pathogens, and is widely used in laboratory pathogen diagnosis. Compared with the virus isolation method, the RT-PCR method is faster and easier to diagnose, and the virus isolation is relatively time-consuming, which is not conducive to rapid clinical diagnosis. At present, the laboratory diagnosis of viral diseases mainly uses the PCR / RT-PCR method. The RT-PCR kits of PRRSV and CSFV have been commercialized and put into clinical application.

[0003] The current multi-virus mixed infection of swine disease is quite serious. Clinically, porci...

Claims

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