Real time-loop-mediated isothermal amplification (RT-LAMP) detection primers for performing differential diagnosis on canine distemper virus wild strains and vaccine strains and application of primers
A technology of RT-LAMP and canine distemper virus, which is applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problem of not being able to identify CDV wild strains and vaccine strains, and achieve high sensitivity , good repeatability and strong specificity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] 1 Materials and methods
[0030] 1.1 Virus strains and materials to be tested
[0031] Asia-I and Asia-II CDV field strains, CDV attenuated vaccine strains, CPV strains, canine adenovirus type I (CAV-I) strains, canine adenovirus type II (CAV-II) strains and rabies virus (RV ) strains were preserved by the Economic Animal Group of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences.
[0032] The materials to be inspected include 56 samples of clinical cases sent for inspection from different farms in Northeast China.
[0033] 1.2 Primer design and synthesis
[0034] Referring to the CDV H gene sequence in GenBank and using the online software Primer Explorer V4 as an aid, RT-LAMP primers were designed, including 2 outer primers F3 / B3 and 2 inner primers FIP / BIP (Table 1).
[0035] Table 1 RT-LAMP primer sequence
[0036]
[0037] 1.3 Viral RNA extraction and cDNA synthesis
[0038] 1.3.1 Extraction of RNA
[0039]Viral RNA was extract...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com