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Method for artificial feeding of Zeuzera leuconolum Butler

A black-spotted leopard moth and six-star technology, applied in animal husbandry and other fields, can solve problems such as long life cycle, inability to obtain insect status at any time, and impossible to obtain larvae for development and utilization of Acremonium acremonium Cordyceps

Inactive Publication Date: 2012-07-25
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because of the long life cycle of the natural six-star black-spotted beetle moth, it is impossible to obtain the insect state required for the experiment at any time, and the experiment is time-consuming and laborious; it is also impossible to obtain a large number of larvae for the development and utilization of Acremonium alternata.

Method used

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  • Method for artificial feeding of Zeuzera leuconolum Butler
  • Method for artificial feeding of Zeuzera leuconolum Butler

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Corn grain medium 35g, sucrose 3g, yeast powder 2.5g, soybean powder 6g, agar 2g, potassium sorbate 0.4g, water 50ml.

[0058] Preparation method: First, mix yeast powder and soybean powder with a small amount of water at 45°C to form a mixed liquid for use; then heat corn kernels with an electromagnetic cooker, boil for 5 minutes, add the above mixed solution, heat to boiling, and continue boiling for 5 minutes; finally add Agar and sucrose, stir quickly, turn off the heat after the agar and sucrose are completely dissolved, add potassium sorbate when the culture temperature drops to 45°C, and stir evenly; put the culture medium in a petri dish, and place it at 4°C after it is completely cooled Store in the refrigerator for later use.

Embodiment 2

[0060] Jujube sawdust medium: 35g jujube sawdust, 4g sucrose, 2.5g yeast powder, 6g soybean powder, 3g agar, 0.4g potassium sorbate, 50ml water.

[0061] Preparation method: Add yeast powder, soybean powder, and sucrose to a small amount of water at 45°C and stir evenly to form a mixed liquid for use; add jujube sawdust and water to boil for 15 minutes on an induction cooker, add the above mixed liquid until boiling, add agar, and stir quickly until After completely dissolving, turn off the heat; when the temperature drops to 50°C, add potassium sorbate, put the culture medium in a petri dish, and store it in a refrigerator at 4°C after it is completely cooled.

Embodiment 3

[0063] Artificial breeding of the six-star black-spotted leopard moth:

[0064] 1. The initial source of insects: the initial source of the six-star black-spotted leopard moth is collected from larvae in the branches of wild host plants.

[0065] 2. The feeding container of the larvae: use a matching petri dish with a diameter of 8-10 cm and a height of 1.5-2 cm to raise. Petri dishes were cleaned and sterilized before feeding.

[0066] 3. Feeding method of larvae:

[0067] A. Divide the prepared artificial feed into cubes with a size of about 4×3×2 cm with a sterilized fruit knife.

[0068] B. Place small pieces of feed and larvae in petri dishes with sterilized tweezers, one piece of feed and one larva in each petri dish, cover with a petri dish, and label it.

[0069] C. Place the petri dish with the larvae connected at a temperature of 24-30°C and a humidity of 55-80%RH.

[0070] D. Change the feed every 6 days on average, and record the values ​​of head width, body leng...

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Abstract

The invention discloses a method for artificial feeding of Zeuzera leuconolum Butler, which includes: selecting breeding containers for an initial insect source and larvae, breeding the larvae, management of pupas, management of imagoes, management of egg hatching and the like. By the method, a great amount of insects of Zeuzera leuconolum Butler which are uniform in development and physiology can be obtained continuously, and the problem of feed deficiency in seasonal host breeding can also be solved. The method is used for artificial feeding of Zeuzera leuconolum Butler for the first time. Tests show that, by the method, artificial feeding of Zeuzera leuconolum Butler can be achieved successfully and the development process of Zeuzera leuconolum Butler can be halved almost.

Description

[0001] This invention is carried out under the support of Tianjin Natural Science Foundation (fund number: 09JCYBJC14500) and Tianjin Normal University doctoral fund (fund number: 52XB1003). technical field [0002] The invention belongs to the technical field of plant protection, and relates to the prevention and research of forestry harmful organisms such as the dry-boring pest Leopard moth, more specifically, a method for artificially raising the moth Leopard moth. Background technique [0003] Six-star black panther moth ( Zeuzera leuconotum Butler) is also known as locust beetle moth, honeysuckle six-star black-spotted beetle moth, walnut leopard beetle moth, white-backed beetle moth, white-backed beetle moth, leopard beetle moth, etc. Belongs to Lepidoptera, Cossidea, Cossidae, Zeuzerinae, Lepidoptera. Known hosts include Chinese pagoda tree, acacia, black locust, amorpha, white wax, lawn, willow, poplar, elm, pine, jujube, persimmon, pear, walnut, hickory, boxwood, ...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 刘强汪敏捷雷玲李敏
Owner TIANJIN NORMAL UNIVERSITY
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