Preparation of novel composite starter for pickled cabbage
A technology of compound starter and starter, applied in the compounding of starter and the field of compound lactic acid bacteria starter
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[0018] 1. Selection of fermentation strains and preparation of culture medium
[0019] Fermentation strains: Leuconostoc membranae, Lactobacillus plantarum and Lactobacillus casei.
[0020] Medium: 10% skim milk medium; modified MRS medium, solid medium supplemented with 2% agar. The culture was based on sterilization at 121°C for 20 min.
[0021] 2. Preparation process of compound sauerkraut starter
[0022] 2.1 Activation of lactic acid bacteria
[0023] Lactobacillus plantarum and Lactobacillus casei were inoculated onto MRS plates for anaerobic culture at 37°C for two days, and a single colony was picked with an inoculating needle and cultured anaerobic at 37°C in 2 mL of 10% skim milk for 12 hours, and then the inoculum amount was 3%. It was inoculated into 8 mL of 10% skim milk and incubated anaerobically at 37°C until curdling, and the curdling time should be less than 10h.
[0024] Leuconostoc enterica was inoculated onto MRS plates for anaerobic culture at 37°C fo...
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