Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Microbes for alkyl glycoside synthesis and application thereof

A technology of alkyl glycosides and microorganisms, which is applied in the field of alkyl glycoside synthesis and can solve the problems of undiscovered glucosidase and the like

Active Publication Date: 2012-08-01
ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching the literature, there is still no report on the use of Arthrobacter glucosidase for the synthesis of alkyl glycosides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbes for alkyl glycoside synthesis and application thereof
  • Microbes for alkyl glycoside synthesis and application thereof
  • Microbes for alkyl glycoside synthesis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Synthesis of methylglucoside catalyzed by Arthrobacter sp.DL001

[0039] The bacteria were inserted into the liquid medium at 120 rpm, cultured at 10°C for 60 hours, and then stored at -20°C.

[0040] The liquid medium used is: dextran 8g, KNO 3 1.2g, K 2 HPO 4 · 3H2O 1.5g, water 1000mL, NaOH to adjust the pH to 6.5-7.0.

[0041] Take 180 μl of the above fermentation broth, add 20 μl of 2% (w / v, the same below) starch solution and 10 μl of methanol, react in a constant temperature water bath at 20°C for 24 hours, then add an equal volume of 200 μl of methanol to terminate the reaction, centrifuge at 15000g*5min*4°C, and place on Qing is the product of transglycosylation.

[0042] UFLC-MS identification was carried out on the supernatant obtained above, and the yield of the obtained product methyl glucoside was about 30%. The equipment and conditions used for the above UFLC-MS analysis: ultra-fast liquid chromatography (UFLC) system including CBM-20A system controll...

Embodiment 2

[0044] The synthesis reaction of n-butyl glucoside catalyzed by Arthrobacter sp.DL001 put the bacteria into the liquid medium at 200rpm, culture at 30°C for 45h, centrifuge the fermentation broth, and take the supernatant, which is the crude enzyme of glycosyltransferase solution, stored at -20°C.

[0045] The liquid medium used is maltose 20g, KNO 3 1g, K 2 HPO 4 ·3H 2 O 0.5g, water 1000mL, NaOH to adjust the pH to 7.4-7.6.

[0046] Take 1.8ml of the above fermentation broth, add 0.1ml of 10% (w / v, the same below) maltose, 0.1ml of n-butanol, react in a constant temperature water bath at 25°C for 5h, then add an equal volume of 2ml of methanol to terminate the reaction, centrifuge at 15000g*5min*4°C Finally, the supernatant is the transglycosylated product.

[0047] UFLC-MS identification was performed on the above-mentioned supernatant, and the equipment and parameters were the same as before. The yield of the obtained product n-butyl glucoside is about 50%.

Embodiment 3

[0049] Synthesis of Isopropylglucoside Catalyzed by Arthrobacter sp.DL001

[0050] Put the bacteria into the liquid medium at 250 rpm, culture at 35°C for 48 hours, centrifuge the fermentation broth, take the supernatant, which is the crude glycosyltransferase enzyme solution, and store it at -20°C.

[0051] The liquid medium used is: glucose 40g, NaNO 3 0.8g, K 2 HPO 4 ·3H 2 O 2g, water 1000mL, NaOH to adjust the pH to 7.0-7.5.

[0052] Take 180 μl of the above fermentation broth, add 10 μl of 20% (w / v, the same below) maltose, 5ul of isopropanol, react in a constant temperature water bath at 30°C for 2 hours, then add an equal volume of 200ul of methanol to terminate the reaction, centrifuge at 15000g*5min*4°C, The supernatant is the transglycosylated product.

[0053] UFLC-MS identification was performed on the above-mentioned supernatant, and the equipment and parameters were the same as before. The yield of the product isopropylglucoside was about 40%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to microbes having a high-efficiency transglycosylation activity and application thereof. The microbes are Arthrobacter sp.DL001. Strains or broth of Arthrobacter sp.DL001 can realize high-efficiency transglycosylation to glycosyl acceptors in the presence of glycosyl donors and thus a novel effective method for preparing carbohydrates and glycosides by biotransformation reactions is provided. The novel effective method can realize high-efficiency, cheap and eco-friendly preparation of carbohydrates and glycosides and is an effective approach of industrial production of carbohydrates and glycosides.

Description

technical field [0001] The present invention relates to the synthesis of alkyl glycosides, specifically a microorganism for the synthesis of alkyl glycosides and its application, which is a kind of microorganism that can transfer the glycosyl in the glycosyl donor to the glycosyl acceptor to form a new A method of glycosidic linkage. Background technique [0002] As a non-ionic surfactant, alkyl glycosides are widely used in the fields of pharmaceuticals, chemicals, cosmetics, detergents, food additives, etc. due to their non-toxic, non-irritating, and easily biodegradable properties, and are internationally recognized as the preferred "green" function Sexual surfactant. [0003] At present, the preparation of alkyl glycosides is mostly a chemical method, mainly using a one-step method or a two-step method. Related patents such as CN1077397, CN1128265, US005585472A, US006166189A, US006100391A, US006077945A, US005886161A, US005859218A, US005696247A, etc. The domestic resear...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/00C12R1/06
Inventor 杨凌周坤栾宏伟
Owner ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com