Extraction method of hederagenin

A technology of hederagenin and an extraction method is applied in the field of extraction of hederagenin to achieve the effects of overcoming incomplete adsorption of impurities, simple operation and short steps.

Inactive Publication Date: 2012-08-15
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the deficiencies of the prior art, to provide a simple, efficient and low-energy-consuming helexin extraction method to solve the problem of large-scale industrial preparation of high-purity hederagenin

Method used

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  • Extraction method of hederagenin

Examples

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Effect test

Embodiment 1

[0016] Prepare a mixed solution of hydrochloric acid and ethanol. First, prepare an ethanol solution with a volume concentration of 45% (v / v), and add hydrochloric acid with an equivalent concentration of 3N to the ethanol solution to obtain a mixed solution of hydrochloric acid and ethanol. Crush 10 kg of traditional Chinese medicine, pass through a 40-mesh sieve, and directly hydrolyze the powder of traditional Chinese medicine with 80 L of the above-mentioned hydrochloric acid and ethanol mixed solution (the weight / volume ratio of material liquid is 1:8). Degrade under reflux conditions for 4 hours until the reaction is complete. After cooling, filter, and wash the insoluble filter residue with water to neutrality. Then the obtained filter residue was extracted twice with ethanol with a volume concentration of 95% (v / v) under reflux conditions, with a dosage of 14 L each time and extraction for 15 minutes. The ethanol solution is combined, and the obtained ethanol extract i...

Embodiment 2

[0018] Prepare a mixed solution of sulfuric acid and ethanol. First, an ethanol solution with a volume concentration of 40% (v / v) is prepared, and sulfuric acid with an equivalent concentration of 2N is added to the ethanol solution to obtain a sulfuric acid-ethanol mixed solution. Crush 10 kg of traditional Chinese medicine, pass a 40-mesh sieve, and directly hydrolyze the powder of traditional Chinese medicine with 100 L of the above-mentioned sulfuric acid and ethanol mixed solution (the weight / volume ratio of material liquid is 1:10). Degrade under reflux conditions for 4 hours until the reaction is complete. After cooling, filter, and wash the insoluble filter residue with water to neutrality. Then the obtained filter residue was extracted twice with ethanol with a volume concentration of 90% (v / v) under reflux conditions, with an amount of 15 L each time and extraction for 15 minutes. The ethanol solution is combined, and the obtained ethanol extract is passed through an...

Embodiment 3

[0020] Prepare a mixed solution of sulfuric acid and ethanol. Firstly, an ethanol solution with a volume concentration of 30% (v / v) is prepared, and sulfuric acid with an equivalent concentration of 1.5N is added to the ethanol solution to obtain a sulfuric acid-ethanol mixed solution. Crush 10 kg of traditional Chinese medicine, pass through a 40-mesh sieve, and directly hydrolyze the powder of traditional Chinese medicine with 120L of the above-mentioned sulfuric acid and ethanol mixed solution (the weight / volume ratio of material liquid is 1:12). Degrade under reflux conditions for 5 hours until the reaction is complete, let cool and filter, and wash the insoluble filter residue with water to neutrality. Then, the obtained filter residue was extracted 3 times with ethanol with a volume concentration of 80% (v / v) under reflux conditions, with an amount of 10 L each time and extraction for 10 minutes. The ethanol solution is combined, and the obtained ethanol extract is passe...

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Abstract

The invention relates to an extraction method of hederagenin, and in the method, Chinese medicinal material teasel root is used as a raw material. The method comprises the following steps: a, adding hydrochloric acid or sulphuric acid into a 30-55% ethanol solution to prepare a 1.5-4N hydrochloric acid or sulphuric acid ethanol mixed solution; b, hydrolyzing the crushed teasel root in the hydrochloric acid or sulphuric acid ethanol mixed solution in the step a, filtering, and washing insoluble residues with water until the insoluble residues are neutral; c, extracting the residues obtained in the step b with an ethanol solution in a refluxing condition to obtain ethanol extract; and d, filtering the ethanol extract obtained in the step c through an ultrafiltration membrane with molecular weight cutoff of 3,000-8,000Da to obtain permeate, filtering the permeate with a nano-filtration membrane with a molecular weight cutoff of 200-600Da to obtain concentrate, and drying the concentrate to obtain the hederagenin. The method is simple and convenient in operation and simple and short in steps, so that the extraction quality and the extraction rate can be greatly improved.

Description

Technical field [0001] The invention relates to an extraction method for extracting hederogenin, a medicinal active ingredient, from natural plants. Background technique [0002] Research data shows that ivy sapogenin has the effects of lowering blood sugar, preventing and treating obesity, lowering blood lipids, and anti-depression (see 1 patent: CN 101125880A; 2 patent: CN 1015664405A; 3 patent: CN 02109763.1; 4 patent: CN 101214250A ), so how to efficiently obtain this compound has become an issue of concern. The content of ivy sapogenin is not abundant in nature, but mostly exists in the form of its saponins. Therefore, if this compound is industrially produced in large quantities, the basic idea is to try to connect the glycoside bonds between sapogenin and different sugar chains. Hydrolyzed and broken to obtain the common skeleton component of different ivy saponins-ivy sapogenin. [0003] [0004] For the extraction of sapogenins from Chinese herbal medicines containing st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 张劲松窦建鹏徐长江孟庆伟李东升张守勤
Owner JILIN UNIV
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