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Magnetic particulate refining liquid

A technology of blocking liquid and magnetic particles, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of easy loss of activity, achieve low cost, easy preparation, and solve the effect of easy loss of activity

Inactive Publication Date: 2012-09-12
AILEX TECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the deficiencies of the prior art, the present invention provides a magnetic particle blocking solution to solve the problem that the activity of magnetic particles is easily lost after coating antibodies or antigens

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The embodiment of the present invention takes alpha-fetoprotein (AFP) monoclonal antibody-coated AFP magnetic particle chemiluminescent immunoassay as an example.

[0019] The AFP magnetic particles were washed and preserved with the magnetic particle blocking solution described below, and placed in a 37°C incubator for accelerated testing. Using the AFP calibrator as a sample, the remaining activity ratio of the AFP antibody in the magnetic particles (the ratio of the activity after the accelerated test to the activity after preparation) was determined by using the Tosoh Co., Ltd. automatic fluorescence analyzer AIA-360.

[0020] Above-mentioned magnetic particle sealing solution, its composition and concentration are:

[0021] Phosphate buffer system 0.02mol / L, pH7.4;

[0022] Bovine serum albumin 10g / L;

[0023] Galactose 0.5g / L;

[0024] Tween-80 0.5ml / L;

[0025] Gentamicin sulfate 500,000 units / L;

[0026] Proclin 300 0.5ml / L.

[0027] From the results in Ta...

Embodiment 2

[0032] The embodiment of the present invention takes alpha-fetoprotein (AFP) monoclonal antibody-coated AFP magnetic particle chemiluminescent immunoassay as an example.

[0033] The AFP magnetic particles were washed and preserved with the magnetic particle blocking solution described below, and placed in a 37°C incubator for accelerated testing. Using the AFP calibrator as a sample, the remaining activity ratio of the AFP antibody in the magnetic particles (the ratio of the activity after the accelerated test to the activity after preparation) was determined by using the Tosoh Co., Ltd. automatic fluorescence analyzer AIA-360.

[0034] Above-mentioned magnetic particle sealing solution, its composition and concentration are:

[0035] Phosphate buffer system 0.02mol / L, pH7.4;

[0036] Bovine serum albumin 5g / L;

[0037] Lactose 0.5g / L;

[0038] Tween-80 0.5ml / L;

[0039] Gentamicin sulfate 500,000 units / L;

[0040] Proclin 300 0.5ml / L.

[0041] From the results in Table...

Embodiment 3

[0046] The embodiment of the present invention takes alpha-fetoprotein (AFP) monoclonal antibody-coated AFP magnetic particle chemiluminescent immunoassay as an example.

[0047] The AFP magnetic particles were washed and preserved with the magnetic particle blocking solution described below, and placed in a 37°C incubator for accelerated testing. Using the AFP calibrator as a sample, the remaining activity ratio of the AFP antibody in the magnetic particles (the ratio of the activity after the accelerated test to the activity after preparation) was determined by using the Tosoh Co., Ltd. automatic fluorescence analyzer AIA-360.

[0048] Above-mentioned magnetic particle sealing solution, its composition and concentration are:

[0049] Phosphate buffer system 0.02mol / L, pH7.4;

[0050] Casein 1g / L;

[0051] Lactose 2.5g / L;

[0052] Tween-80 0.5ml / L;

[0053] Gentamicin sulfate 5 million units / L;

[0054] Proclin 300 0.5ml / L.

[0055] From the results in Table 3, it can b...

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PUM

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Abstract

The invention relates to a pharmaceutical reagent, in particular to magnetic particulate refining liquid. The magnetic particulate refining liquid comprises 0.02mol / L of buffer solution with the pH (potential of hydrogen) of 7.4, 0,5-10g / L of protein substances, 0.5-30g / L of carbohydrate, 0.2-2g / L of surfactant, 0.1-2g / L of Proclin 300 and 5000000-50000000 units / L of gentamicin. Compared with the prior art, the magnetic particulate refining liquid is a frequently-used reagent, not only is prepared easily, low in cost and widely applicable to various products for chemiluminiscence in-vitro diagnosis, but also solves the problem that the activity of an antibody or an antigen is easy to lose after the antibody or the antigen is enveloped by magnetic particles.

Description

[technical field] [0001] The invention relates to a medical reagent, in particular to a magnetic particle sealing solution. [Background technique] [0002] With the improvement of living standards, health problems have increasingly become the focus of people's attention, which has brought about the vigorous development of clinical immunodiagnosis. At present, the immunoassays used clinically in China mainly include immunoturbidimetry, enzyme-linked immunodiagnosis (EIA), chemiluminescence immunodiagnosis (CLIA), etc. Among them, a new platform in the most widely used chemiluminescence immunodiagnosis (CLIA)— —Magnetic particle chemiluminescence, especially showing a strong momentum of development. Antigens or antibodies can easily lose their activity after being coated on magnetic particles. Therefore, ensuring the stability of magnetic particles has become one of the key technologies for the development of this platform. [Content of the invention] [0003] In order to o...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/577
Inventor 李子樵崔京京
Owner AILEX TECH GRP CO LTD
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