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Heat-resistant freeze-drying protective agent for newcastle disease live vaccines and preparation method

A heat-resistant freeze-drying and Newcastle disease technology, which is applied in antiviral agents, pharmaceutical formulas, medical preparations with non-active ingredients, etc., can solve the problems of long-term storage and transportation, and achieve high aging resistance and low virus loss rate Effect

Inactive Publication Date: 2012-09-19
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The invention provides a heat-resistant freeze-drying protective agent for Newcastle disease live vaccine, the purpose of which is to mix the heat-resistant freeze-drying protective agent of the vaccine with the live vaccine to solve the problem that the live Newcastle disease vaccine can only be stored and transported at 2°C to 8°C for a long time question

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of Heat-resistant Freeze-dried Vaccine of Chicken Newcastle Disease Live Vaccine

[0025] 1) Mix carboxymethyl cellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare a concentration of 5% (the concentration involved in the present invention is all unless otherwise specified. is the mass percent concentration) of liquid A.

[0026] 2) Mix xylo-oligosaccharides, sorbitol and water for injection to prepare liquid B, the concentration of xylo-oligosaccharides is 12%, and the concentration of sorbitol is 3%.

[0027] 3) Mix polyvinylpyrrolidone, glutamine, sodium selenite, and water for injection, stir and mix well, and prepare liquid C, polyvinylpyrrolidone 3%, glutamine concentration 1.5%, sodium selenite The concentration is 1.5%.

[0028] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-dried protective agent.

[0029] 5) Mix the vaccine lyoprotecta...

Embodiment ２

[0033] Preparation of Heat-resistant Freeze-dried Vaccine of Chicken Newcastle Disease Live Vaccine

[0034] 1) Mix carboxymethylcellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare liquid A with a concentration of 3%.

[0035] 2) Mix xylooligosaccharides, sorbitol and water for injection to prepare liquid B, the concentration of xylooligosaccharides is 6%, and the concentration of sorbitol is 9%.

[0036] 3) Mix polyvinylpyrrolidone, glutamine, sodium selenite, and water for injection, stir and mix well, and prepare liquid C, polyvinylpyrrolidone 3%, glutamine concentration 1.5%, sodium selenite The concentration is 0.3%.

[0037] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-dried protective agent.

[0038] 5) Mix the vaccine lyoprotectant with the live Newcastle disease vaccine virus solution at a volume ratio of 4:1.

[0039] 6) Freeze-dry after aliquoting. ...

Embodiment ３

[0042] Preparation of Heat-resistant Freeze-dried Vaccine of Chicken Newcastle Disease Live Vaccine

[0043] 1) Mix carboxymethylcellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare liquid A with a concentration of 6%.

[0044] 2) Mix xylo-oligosaccharides, sorbitol and water for injection to prepare liquid B, the concentration of xylo-oligosaccharides is 10%, and the concentration of sorbitol is 3%.

[0045] 3) Mix polyvinylpyrrolidone, glutamine, sodium selenite with water for injection, stir and mix well, and prepare liquid C with 3% polyvinylpyrrolidone, 2.4% glutamine, and sodium selenite The concentration is 1.2%.

[0046]4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-dried protective agent.

[0047] 5) Mix the vaccine lyoprotectant with the live Newcastle disease vaccine virus solution at a volume ratio of 5:1.

[0048] 6) Freeze-dry after aliquoting.

[...

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PUM

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Abstract

The invention relates to a heat-resistant freeze-drying protective agent for newcastle disease live vaccines and a preparation method. The heat-resistant freeze-drying protective agent for the newcastle disease live vaccines specifically comprises the following components in percentage by weight: 1-2% of sodium carboxymethylcellulose, 2-8% of xylo-oligosaccharide, 1-3% of sorbitol, 1-3 %of polyvinylpyrrolidone, 0.5-0.8% of glutamine, 0.1-0.5% of sodium selenite and the balance of water for injection. The technical problem that the newcastle disease live vaccines cannot be stored and transported for a long time at the temperature of 2 DEG C to 8 DEG C is solved by the protective agent provided by the invnetion.

Description

[0001] technical field [0002] The invention relates to a heat-resistant freeze-drying protective agent for chicken Newcastle disease live vaccine and a preparation method thereof, belonging to the field of animal vaccine production. [0003] Background technique [0004] Newcastle disease (ND), also known as Asian chicken plague or pseudo chicken plague, is an acute, highly contagious and fatal infectious disease of poultry caused by Newcastle disease virus (NDV), which is widely prevalent in many countries in the world. It is characterized by dyspnea, diarrhea, nervous disorders, mucosal and serosal hemorrhage, which seriously endangers the poultry industry in the world and is listed as a class A infectious disease by the World Health Organization (OIE). [0005] Newcastle disease live vaccines are generally prepared by inoculating 9-11-day-old chicken embryos with chicken ND vaccine virus, continuing to hatch for 4-5 days, amplifying the virus, and then collecting allan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/38A61K47/26A61K47/04A61K39/17A61P31/14
Inventor 郝智慧曲宝涵苑国春丁兆鹏杨芬芳
Owner QINGDAO AGRI UNIV
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