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Application of Lactobacillus johnsonii to improvement of weaned pig production performance

A Lactobacillus johnsonii, weaned piglet technology, applied in the application, animal feed, animal feed, etc.

Inactive Publication Date: 2012-10-03
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no product in China that uses Lactobacillus johnsonii as an additive to add food and feed additives, and its application in animal and poultry breeding is rarely reported. Lactobacillus johnsonii is developed into an animal microecological feed additive. Filling in the domestic gap and screening Lactobacillus johnsonii with excellent probiotic properties is of great significance to promoting the level of livestock and poultry breeding in my country, and can play a greater role in livestock and poultry breeding

Method used

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  • Application of Lactobacillus johnsonii to improvement of weaned pig production performance
  • Application of Lactobacillus johnsonii to improvement of weaned pig production performance
  • Application of Lactobacillus johnsonii to improvement of weaned pig production performance

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Experimental program
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Effect test

Embodiment 1

[0035] The preparation of embodiment 1 Lactobacillus johnsonii CGMCC No.4926 freeze-dried agent

[0036] 1) Activate cryopreserved Lactobacillus johnsonii CGMCC No.4926 on the MRS agar plate for three times, pick a single colony and inoculate it into a 10mL deep layer MRS liquid test tube, culture it at 37°C for 10 hours, then transfer it to 250mL MRS with 1% inoculum Liquid medium, used as seed liquid after culturing for 10 hours;

[0037] 2) Inoculate the seed solution obtained in step 1) in a 5L fermenter with an inoculum size of 5%;

[0038] 3) During the fermentation process, the pH value of the fermentation broth is maintained at 6.0. When the pH exceeds the set value, the feed liquid is added; when the pH is lower than the set value, the alkaline neutralizer ammonia water is automatically added; Ferment for 14 hours under the condition of 100rpm, N 2 , to maintain dissolved oxygen less than 1%;

[0039] 4) When the pH of the feed liquid no longer decreases or the abs...

Embodiment 2

[0043] (1) Experimental animals and experimental design

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Abstract

The invention discloses application of Lactobacillus johnsonii (preservation No.: CGMCC No.4926) to improvement of weaned pig production performance. After a proper amount of Lactobacillus johnsonii is added into feed, the effects of reducing weaned pig feed to gain (F:G) and improving weaned pig feed conversion rate are remarkable.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of a strain of Lactobacillus johnsonii in improving the production performance of weaned piglets. Background technique [0002] Lactobacillus johnsonii belongs to Firmicutes, Bacilli, Lactobacillales, Lactobacillaceae, Lactobacillus, and was first isolated in 1980. It has been classified as Lactobacillus acidophilus in science. In 1992, Fujisawa et al. named it Lactobacillus johnsonii through biochemical experiments and DNA hybridization experiments. Lactobacillus johnsoni mainly exists in the digestive tract of animals and humans, and is the dominant flora. [0003] The European Food Safety Authority (EFSA) issued two health declarations against Lactobacillus johnsonii in 2009 and 2011 respectively, stating that Lactobacillus johnsonii has microorganisms that reduce intestinal pathogens, enhance natural defense and immune system and maintain healthy sk...

Claims

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Application Information

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IPC IPC(8): A23K1/16
Inventor 张军汪攀孙晓雯闫轶洁王安如
Owner BEIJING DABEINONG TECH GRP CO LTD
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