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Application of black raspberry extract (BRBE) in preparation of drugs for treating gastric cancer

An extract, technology of black raspberry, applied in the field of medicine, can solve problems such as no report of black raspberry or its extract

Active Publication Date: 2012-10-03
赵红石
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art, there is no relevant report of black raspberry or its extracts in the treatment of cancer, especially gastric cancer

Method used

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  • Application of black raspberry extract (BRBE) in preparation of drugs for treating gastric cancer
  • Application of black raspberry extract (BRBE) in preparation of drugs for treating gastric cancer
  • Application of black raspberry extract (BRBE) in preparation of drugs for treating gastric cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: the preparation of BRBE

[0016] This example provides two methods for preparing BRBE.

[0017] (1) The black raspberry fruit is crushed by a masher, and then filtered through a 0.02-inch aperture sieve to remove seeds and make a puree. Strain the remaining fruit and repeat this operation a further three times to ensure adequate juice extraction. Juicing rate is 97%. The prepared puree was poured into a 1-inch freeze-drying dish coated with polyethylene film, and stored in a ventilated freezer. Further freeze-drying is carried out by a sublimator to obtain black raspberry fruit freeze-dried powder. The freeze-dried powder of black raspberry fruit was added to petroleum ether and then ultrasonically extracted twice. The ultrasonic extraction temperature was 25 ℃; the time was 30 min; the mass ratio of petroleum ether to black raspberry fruit freeze-dried powder was 4:1; The ultrasonic extracts were combined, and the solvent was recovered under reduced ...

Embodiment 2

[0019] Example 2: BRBE inhibits the growth of human gastric cancer cells in vitro

[0020] Using 5 kinds of human malignant gastric tumor (SGC7901, MNK45, BGC8223, HCG27, GC9811) cell lines, the in vitro anti-cancer activity of BRBE was determined by MTT method, the concentration range was 0-500 mg / ml, and the treatment time was 72 hours. Table 1.

[0021]

[0022] It can be seen from Table 1 that BRBE can inhibit the growth of human gastric cancer cells in vitro, and the sensitivity to BRBE is significantly different among different cell lines.

Embodiment 3

[0023] Example 3: BRBE combined with 5-FU inhibits the growth of human gastric cancer cells in vitro

[0024] Using 5 kinds of human malignant gastric tumor (SGC7901, MNK45, BGC8223, HCG27, GC9811) cell lines, the in vitro anticancer activity of BRBE alone and in combination with 5-FU was determined by MTT method, and the treatment time was 72 hours. The results are shown in the table 2.

[0025]

[0026] It can be seen from Table 2 that after combined administration, BRBE can significantly reduce the IC50 value of 5-FU in different cell lines.

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Abstract

The invention relates to an application of a black raspberry extract (BRBE) in preparation of drugs for treating gastric cancer. According to the invention, the BRBE can be mixed with a pharmaceutical excipient to prepare a clinically acceptable anti-tumor preparation used for treating gastric cancer, and the total effective dose of the BRBE is 0.5-1g / kg / d. Found by anti-cancer experiment researches of in vitro various human gastric tumor cells and in vivo tumor animal models, the BERE has a better inhibitory action on the in vitro tumor cells and a quite obvious effect on preventing and treating the gastric cancer.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the medical application of black raspberry extract (BRBE). Background technique [0002] Gastric cancer is one of the most common malignant tumors in my country, and its incidence rate ranks first among all kinds of tumors in my country. The incidence rate of gastric cancer in China is highest in Northwest China, followed by Northeast China, Inner Mongolia, East China and coastal areas, and lowest in Central South and Southwest China. About 170,000 people die from gastric cancer every year, nearly 1 / 4 of all malignant tumor deaths, and there are more than 20,000 new patients every year. Gastric cancer seriously threatens the physical and mental health of human beings and brings a heavy burden to patients (families), society and the country. [0003] Gastric cancer can occur at any age, but it is more common in 40-60 years old, and the number of men is about 2:1. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P35/00A61K131/00
Inventor 毕秀丽赵余庆杨万才
Owner 赵红石
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