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Application of amomum tsao-ko oil on preparing medicaments with functions of restraining or killing candida albicans

A technology for Candida albicans and candidiasis, which is applied in the field of medicine and health, can solve the problems of drug resistance of Candida and large side effects, and achieves the effects of exact curative effect, good antibacterial and bacteriostatic effect, and good clinical application prospect.

Inactive Publication Date: 2012-10-10
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibiotics and azole anti-candida drugs have the characteristics of broad antifungal spectrum, rapid and complete oral absorption, and high blood-brain barrier permeability, but their side effects are large, and widespread use will cause Candida to develop drug resistance

Method used

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  • Application of amomum tsao-ko oil on preparing medicaments with functions of restraining or killing candida albicans
  • Application of amomum tsao-ko oil on preparing medicaments with functions of restraining or killing candida albicans
  • Application of amomum tsao-ko oil on preparing medicaments with functions of restraining or killing candida albicans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of grass fruit oil of the present invention

[0022] Take the grass fruit, crush it through a 20-40 mesh sieve, add 10-14 times the weight of distilled water, soak for 1-5 hours, and extract for 2-6 hours by steam distillation to obtain the grass fruit oil of the present invention.

[0023] The grass fruit oil of the present invention can also be extracted according to the volatile oil determination method in Appendix XD of the Pharmacopoeia (2005 edition), and can also be extracted by using existing technologies such as organic solvent extraction, supercritical CO2 extraction, or by purchasing commercially available products.

[0024] The following pharmacodynamic tests prove the beneficial effects of the present invention:

[0025] Materials and equipment

[0026] 1.1 Experimental strains

[0027] ①Standard strain: Candida albicans standard strain (ATCC14053), from the American Clinical Laboratory Culture Collection; Candida albicans standard strain (CICC32...

experiment example 1

[0039] Experimental Example 1 In vitro experiment of anti-candida albicans of grass fruit oil of the present invention

[0040] 1 Test method

[0041] Preparation of medicated plates: the herb fruit oil of the present invention was diluted by the multiple dilution method, and diluted into 11 gradients of 1:2~1:1024, respectively, and the medicinal liquids of different concentration gradients were added to the disposable sterile petri dish. 1ml and 14ml of sterilized Sabouraud agar medium, that is, the dilution gradient of each drug in the plate is 30.70mg / ml~59.95μg / ml, mix well, dry and set aside, add the same amount of saline to the plate Instead of drugs, prepare positive control plates.

[0042] Bacterial solution configuration: Adjust the standard strain of Candida albicans and clinical isolates with sterile physiological saline to the concentration of bacterial solution 1.5×10 6 CFU / ml.

[0043] Determination of the minimum inhibitory concentration (MIC): use a multi-point inoc...

experiment example 2

[0052] Experimental example 2 The in vivo activity of the present invention's grass fruit oil in treating fungal vaginitis caused by Candida albicans

[0053] 1 Experimental method

[0054] (1) Establishment of a mouse model of fungal vaginitis caused by Candida albicans

[0055] Activation of the tested bacteria: Inoculate the standard Candida albicans strain CICC32819 on Sabouraud agar medium, culture at 37°C for 48 hours, and activate it for later use.

[0056] Model preparation: Through pre-experiment, various factors affecting the establishment of the model were optimized, and the method for establishing the model of fungal vaginitis caused by Candida albicans was determined. In the formal experiment, 70 SPF Kunming mice with a body weight of (20±2) g were selected, of which 60 were started 6 days before infection, and each mouse was injected subcutaneously with 1 mg / ml estradiol benzoate oil 0.1 on the back every other day ml, and inoculate Candida albicans 1.5×10 after the las...

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Abstract

The invention discloses application of amomum tsao-ko oil on preparing medicaments with functions of restraining or killing candida albicans. The invention further discloses a medicine composite with the functions of restraining or killing the candida albicans. The medicine composite enables the amomum tsao-ko oil to serve as an active ingredient, and auxiliary materials or auxiliary ingredients which are acceptable on the pharmacy are added to prepare. The amomum tsao-ko oil has the functions of restraining or killing the candida albicans, can treat candida albicans diseases, and has strong practical application value.

Description

Technical field [0001] The invention relates to a new use of grass fruit oil and belongs to the field of medicine and hygiene. Background technique [0002] Candida Albicans, also known as Candida albicans, is widespread in nature and is also one of the normal human flora. It usually lives in the oral cavity, skin, mucous membrane, digestive tract, vagina and other organs of the human body. Candida albicans is a conditional pathogenic fungus, and its pathogenicity is relative. Whether it develops depends on the level of human immunity and the number and virulence of infectious bacteria. In a normal body, they are small in number, are oval in shape, are in a symbiotic state with the body, and do not cause disease. When the body's resistance or immunity is reduced under the influence of certain physiological and pathological factors, the balance state is destroyed, and Candida albicans transforms from the yeast phase to the hyphae phase, and grows and multiplies locally, causing s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9064A61P31/10A61P15/02C11B9/02
Inventor 彭成代敏曹小玉王蓬勃
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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