Active immunoregulation method for fused prolactin proteins of goose eggs

An active immunization, prolactin technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, drug combinations, etc., can solve the problems of diluting good quality, strong nesting, low egg production, etc., to solve the problem of difficult to eliminate The effect of nesting traits, avoiding drug residues, and improving reproductive performance

Inactive Publication Date: 2012-10-10
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

Chinese geese lay eggs seasonally, but their nesting ability is extremely strong, resulting in low egg production
Using varieties with high egg production as the female parent to carry out hybridization can not meet the needs of l

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  • Active immunoregulation method for fused prolactin proteins of goose eggs

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Embodiment Construction

[0019] The present invention will be further described in detail according to the examples below.

[0020] A method for active immune regulation of goose egg-laying fusion prolactin protein, the steps comprising:

[0021] (1) Through RT-PCR, the full-length PRL target sequence was amplified from the total RNA extracts of goose hypothalamus and pituitary tissue, and inserted into EcoRI and HindIII restriction sites;

[0022] (2) Re-insert into the PMD19-T vector, digest and ligate with pET32a, and transform into DH5α;

[0023] (3) Use IPTG to induce for 3 hours, express in secreted form, and adjust the concentration of the expressed protein product after induction to 300-350 mg / L;

[0024] (4) After induced expression, the bacterial beads were crushed by high-pressure ultrasonication, and the supernatant solution was passed through a Ni-affinity chromatography column. The purified product was dialyzed and then freeze-dried and concentrated to obtain the fusion prolactin expres...

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Abstract

The invention discloses an active immunoregulation method for fused prolactin proteins of goose eggs, which comprises the simple steps of extraction, enzyme digestion, induction, purification, concentration, mixing and injection. The active immunoregulation method has the advantages that recombinant expression fused prolactin proteins are applied to active immunity of gooses, and the reproductive performance of the gooses is improved through regulating the body's hormone level, so that the difficult problem that in the conventional breeding, broody traits are difficult to eliminate is solved, and meanwhile, the problem that drug residue caused by injecting sex hormone substances by people is also avoided.

Description

technical field [0001] The invention relates to a method for active immune regulation of goose egg-laying fusion prolactin protein. Background technique [0002] Most of the Chinese geese are white-feathered medium-sized goose breeds. They are famous at home and abroad for their large size, fast early growth and development, strong disease resistance, resistance to rough feeding, low feed consumption, and good meat quality. . Chinese geese lay eggs seasonally, but their nesting ability is extremely strong, resulting in low egg production. Using varieties with high egg production as the female parent to carry out hybridization can not meet the needs of local production and market for products in terms of its comprehensive production performance, and it also dilutes the original good quality of local goose varieties, which affects the quality of goose breeding. economic benefits. Therefore, eliminating nesting is the most effective way to improve the reproductive performanc...

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Application Information

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IPC IPC(8): A61K39/00A61P43/00C12N15/70C12R1/19
Inventor 耿照玉陈兴勇何艳姜润深时丹
Owner ANHUI AGRICULTURAL UNIVERSITY
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