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Pharmaceutical composition providing inhibition effect against inflammation factor expression

A technology of inhibition and inflammatory factors, applied in the field of medicine, can solve the problems of large dosage of original prescription and difficulty of new drug development, etc.

Active Publication Date: 2012-10-17
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Simiao Yong'an Decoction" is one of the traditional famous prescriptions with the most characteristics of compatibility and dosage in traditional Chinese medicine. It has a good prospect for the development of patented medicines. Its compatibility rules and dose-effect relationship are also worthy of further study. However, due to the excessive dosage of the original prescription, it is difficult to develop new drugs

Method used

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  • Pharmaceutical composition providing inhibition effect against inflammation factor expression
  • Pharmaceutical composition providing inhibition effect against inflammation factor expression
  • Pharmaceutical composition providing inhibition effect against inflammation factor expression

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0026] Experimental Example 1 Inhibitory Effect of a Single Extract on the Binding of NF-κB Response Elements

[0027] 1. Experimental steps

[0028](1) Experimental materials: NF-κB-RE cells (pGL4.32[luc2P / NF-kappaB-RE]293A, Institute of Materia Medica, Chinese Academy of Medical Sciences), MEM-α medium (Gibco), fetal bovine serum (Gibco) , 96-well culture plate (Costar, 3599), Bright-Glo Luciferase detection reagent (Promaga), water-jacketed CO 2 Cell incubator (Sanyo, Japan), Safire2 high-speed multi-channel continuous wavelength microplate reader (TECAN, Austria).

[0029] (2) Test samples:

[0030] Extracts 1-3 obtained by the method described in Example 1 were provided by Beijing University of Traditional Chinese Medicine.

[0031] (3) Experimental steps:

[0032] Digest and count the logarithmically grown NF-κB-RE cells in the culture flask, and use 100 μl system, 5×10 3 / well density inoculated in 96-well white culture plate, incubated for 18-24h. The control gro...

experiment example 2

[0041] Experimental Example 2 The agonistic effect of a single extract on peroxisome proliferator receptor (PPARγ)

[0042] 1 material

[0043] 1.1 Reagents:

[0044] African green ape kidney cells (CV-1), PPRE plasmid, PPAR plasmid, RXRα plasmid, MEM-α medium (Gibco), fetal bovine serum (Gibco), Bright-Glo Luciferase luciferase detection reagent (Promega) , rosiglitazone.

[0045] 1.2 Instruments:

[0046] Water-jacketed CO2 cell incubator (Sanyo, Japan), EnSpireTM 2300MultilabelReader (PerkinElmer).

[0047] 2 methods

[0048] 2.1 Extraction of the test product

[0049] Three extracts obtained by the method described in Example 1 were provided by Beijing University of Traditional Chinese Medicine.

[0050] 2.2 Cell Culture

[0051] African green ape kidney cells (CV-1) were cultured in DMEM medium supplemented with 10% FBS and 1% antibiotics (penicillin / streptomycin) in 5% CO 2 Cultured in an incubator at 37°C.

[0052] 2.3 Transfection and luciferase gene expressio...

experiment example 3

[0063] Experimental Example 3 Study on the Combination and Inhibitory Effect of Extracts on NF-κB Response Elements

[0064] 1. Experimental steps

[0065] Same as Experiment 1.

[0066] 2. Orthogonal design

[0067] The research on the compatibility law adopts 3 factors and 3 levels of orthogonal experimental design, according to the orthogonal table, a total of 27 compatibility groups are designed. Among them, the three factors are the first extract, the second extract, and the third extract, and the concentration points of the three levels are taken within the range of the active dose-effect relationship of each active site. The factor levels are shown in Table 3.

[0068] table 3

[0069] (μg / ml) 1st extract 2nd extract 3rd extract 1 69 54 51.6 2 34.5 27 25.8 3 17.25 13.5 12.9

[0070] 3. Experimental results

[0071] See Table 4 for detailed results.

[0072] Table 4 The effect of each compatibility gr...

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PUM

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Abstract

The invention provides a pharmaceutical composition providing an inhibition effect against inflammation factor expression. The pharmaceutical composition is composed of a first extract, a second extract and a third extract. The preparation method of the extracts comprises the steps that: raw materials of honeysuckle flower, figwort, Chinese angelica and licorice are well mixed according to a weight ratio of 2.5-4:2.5-4:1.5-2.5:0.5-1.5; the mixture is subjected to ethanol extraction, such that an extraction liquid is obtained; the extraction liquid is condensed, and is loaded on macroporous resin; 30% ethanol, 60% ethanol, and 95% ethanol are respectively used for eluting, such that the first extract, the second extract and the third extract are obtained; the extracts are mixed, such that the pharmaceutical composition is obtained. The pharmaceutical composition provided by the invention can effectively inhibit inflammation factor expression.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the effective part of traditional Chinese medicine and its application field, in particular to a composition of the effective part of traditional Chinese medicine which can inhibit the expression of inflammatory factors. Background technique [0002] Inflammatory injury is a complex reaction involving the extensive participation of the immune system when organs such as the heart and brain are injured. During the inflammatory process, a large number of inflammatory factors, including cytokines and adhesion molecules, are released to promote further damage. The occurrence of inflammatory response will lead to the occurrence of secondary damage to local organs, such as cerebral ischemia and myocardial ischemia, which are common clinical diseases closely related to inflammatory response. [0003] For example, acute coronary syndrome (ACS) is a group of clinical syndromes caused by acute myoca...

Claims

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Application Information

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IPC IPC(8): A61K36/808A61P29/00
Inventor 陈立新吴圣贤聂波
Owner BEIJING UNIV OF CHINESE MEDICINE