Method for rapidly enriching astaxanthin and ester thereof from Euphausia superba

A technology of Antarctic krill and astaxanthin, applied in the direction of organic chemistry, can solve problems such as complex process, achieve good effect and fast adsorption effect

Inactive Publication Date: 2012-10-17
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To prepare astaxanthin and its esters, it is necessary to use other solvents or other methods for further extraction and enrichment, and the process is relatively complicated regardless of laboratory preparation or industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Add 100g of dried Antarctic krill, add 600ml of n-hexane, stir and extract for 3 hours, pour out and use an 80-mesh filter to filter the extract, repeat the extraction for 2 times in the same way, a total of 3 times, combine the coarse filtrate and fine filter to obtain the filtrate .

[0022] Take 1000ml filtrate, add 20gV to it E 50% white V E Powder, stir for 5 seconds, white V is visible E The powder turns red quickly, while the solution is white due to V E The rapid adsorption of the powder changed from red to light yellow. After the liquid has stood still, pour the liquid out to get a red V E pink. to the red V E Add 3 to 5 times acetone (V) to the powder (V), stir and elute for 5 to 10 minutes, filter, repeat the same elution method twice, combine the eluents to obtain a red acetone liquid, evaporate the acetone to obtain a red paste matter (filtered white V E Powder content less than 50% V E powder, dried to 17.5g). Dissolve the paste in petroleum ethe...

Embodiment 2

[0024] The n-hexane extraction method is the same as in Example 1, and 2g of white V is added to the 1000ml filtrate. E Stir the powder, the red color in the liquid is quickly replaced by V E The powder absorbs and turns red, and the liquid is poured out to get a red V E Powder, after elution with acetone, elution by column chromatography and detection by thin-layer analysis, it is known that astaxanthin is mainly adsorbed, astaxanthin accounts for 90% and astaxanthin ester accounts for about 10%, and astaxanthin is obtained in total and astaxanthin ester 0.55g.

Embodiment 3

[0026] White V E The solvent used in the preparation of the Antarctic krill extract before the powder adsorption was 6# gasoline, and the others were the same as in Example 1 to obtain 0.53 g of astaxanthin and astaxanthin ester.

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Abstract

The method discloses a method for rapidly enriching astaxanthin and ester of the astaxanthin from Euphausia superba, which comprises the steps of: adding white vitamin E (VE) powder into n-hexane, cyclohexane, petroleum ether or 6# gasoline extract of dry Euphausia superba, stirring and adsorbing for 5s for adsorbing the astaxanthin and the ester of the astaxanthin in the extract; eluting the red VE powder obtained by separation by acetone until the VE powder becomes white to obtain red paste; treating red VE dried paste by silica gel column chromatography to obtain astaxanthin ester eluate and VE eluate; respectively evaporating the astaxanthin ester eluate and the VE eluate for removing solvent to obtain astaxanthin ester and VE liquid, wherein the part which does not move downwards during column top elution is the astaxanthin; and taking out the astaxanthin part, extracting by acetone, and evaporating for removing acetone to obtain the astaxanthin. The method for rapidly enriching the astaxanthin and the ester of the astaxanthin from the Euphausia superba is rapid in adsorption and good in effect, and can be applied to a laboratory or industrial production.

Description

technical field [0001] The invention relates to a method for rapidly enriching astaxanthin and its esters from Antarctic krill. Background technique [0002] Antarctic krill (Euphausia superba), also known as giant krill, lives in the waters of Antarctica in the Southern Ocean. Live in clusters, sometimes at a density of 10,000 to 30,000 / m 3 . They are probably the most successful animal species on Earth, at about 500-5000000000 tons (varies greatly according to different methods of estimation). The Antarctic krill fishery is approximately 100,000 tonnes per year. The main fishing countries are Japan and Poland. It is mainly used as cooking in Japan, and as animal feed and fishing bait in other parts of the world. [0003] More than 90% of natural astaxanthin exists in esterified form, which is relatively stable. The preparation, storage and application of most products are carried out in the form of astaxanthin ester. Antarctic krill contains astaxanthin and astaxant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
Inventor 刘代成孙晓昀
Owner SHANDONG NORMAL UNIV
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