Probiotic goat milk tablet and preparation method of probiotic goat milk tablet
A technology for probiotics and goat milk tablets is applied in the field of probiotics goat milk tablets and their preparation, which can solve the problems of single variety of probiotics, and achieve the effects of promoting proliferation, eliminating carcinogenic factors and improving body immunity.
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[0026] Example 1:
[0027] 1) First, the probiotics Lactobacillus acidophilus and Lactobacillus plantarum were activated at 37°C with MRS liquid medium three times to obtain activated probiotics, and the activated probiotics Lactobacillus acidophilus and Lactobacillus planta % Of the inoculum was connected to a 10L MRS medium fermenter and cultured for 18 hours. During the culture, it was stirred every 3 hours at a rotation speed of 60 rpm for 2 minutes. Then, centrifuged at 8000 rpm / min for 12 minutes. Prepare probiotic bacteria mud, then add 1mL of composite freeze-dried protective agent per g probiotic mud to the probiotic bacteria mud, mix it evenly with a vortex mixer, pre-freeze at -40°C for 12h, and then move to the freeze dryer Freeze drying for 24 hours to obtain freeze-dried probiotic powder;
[0028] The composite freeze-dried protective agent is composed of 18% skimmed milk powder, 20% trehalose, 2% disodium hydrogen phosphate, 10% lactose and 50% phosphate buffer by m...
Example Embodiment
[0031] Example 2:
[0032] 1) First, the probiotics Lactobacillus acidophilus and Lactobacillus rhamnosus were activated at 37°C with MRS liquid medium three times to obtain activated probiotics, and the activated Lactobacillus acidophilus and Lactobacillus rhamnosus were taken Insert 5% of the inoculum into a fermentation tank with 10L MRS medium and culture for 18 hours. Stir every 4 hours during the culture process at a rotation speed of 60 revolutions / min for 2 minutes. Then, use a centrifuge at 8000 revolutions / min. Centrifuge for 12 min to make probiotic bacteria paste, then add 1 mL of composite freeze-dried protective agent per g of probiotic paste to the probiotic bacteria paste, mix well with a vortex mixer, pre-freeze at -30°C for 18 hours, and then move to freezing Freeze drying in a dryer for 24 hours to obtain freeze-dried probiotic powder;
[0033] The composite freeze-dried protective agent is composed of 50% skimmed milk powder, 8% trehalose, 1% disodium hydrogen ...
Example Embodiment
[0036] Example 3:
[0037] 1) First, the probiotic Lactobacillus acidophilus was activated three times with MRS liquid medium at 37°C to obtain activated Lactobacillus acidophilus, and then Bifidobacterium infantis was added to the MRS liquid medium with 0.05% of the medium volume The cysteine hydrochloride was activated three times with MRS liquid medium under 37°C culture conditions, and each time was transferred with 5% inoculum. The first two times were cultured at 37°C for 20h, and the third time Cultivate at 37℃ for 18 hours, then perform microscopic examination and measure the OD value. After confirming that there are no bacteria, activate it for the third time to obtain activated Bifidobacterium infantis, and take the activated probiotic Lactobacillus acidophilus and Bifidobacterium infantis 5% of the inoculum was connected to the fermentor with 10L MRS medium (5g of cysteine hydrochloride added to the MRS medium) and cultured for 18h. During the cultivation, it was s...
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