Probiotic goat milk tablet and preparation method of probiotic goat milk tablet
A technology for probiotics and goat milk tablets is applied in the field of probiotics goat milk tablets and their preparation, which can solve the problems of single variety of probiotics, and achieve the effects of promoting proliferation, eliminating carcinogenic factors and improving body immunity.
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Embodiment 1
[0027] 1) First, the probiotics Lactobacillus acidophilus and Lactobacillus plantarum were respectively activated three times in MRS liquid medium at 37°C to obtain activated probiotics, and the activated probiotics Lactobacillus acidophilus and Lactobacillus plantarum were taken at 5 % of the inoculum was inserted into a 10L MRS medium fermenter for 18 hours, stirred once every 3 hours during the cultivation process, the speed was 60 rpm, and the stirring time was 2 minutes, and then centrifuged with a centrifuge at a speed of 8000 rpm for 12 minutes Make probiotic slime, then add 1mL of compound freeze-drying protective agent per gram of probiotic slime to the probiotic slime, mix evenly with a vortex mixer, pre-freeze at -40°C for 12 hours, and then transfer to a freeze dryer Freeze-dried for 24 hours to obtain freeze-dried probiotic bacteria powder;
[0028] The compound lyoprotectant consists of 18% skimmed milk powder, 20% trehalose, 2% disodium hydrogen phosphate, 10% l...
Embodiment 2
[0032] 1) First, the probiotics Lactobacillus acidophilus and Lactobacillus rhamnosus were respectively activated three times in MRS liquid medium at 37°C to obtain activated probiotics, and the activated Lactobacillus acidophilus and Lactobacillus rhamnosus were taken Insert 5% of the inoculum into a 10L MRS medium fermenter for 18 hours, and stir once every 4 hours during the cultivation process at a speed of 60 rpm for 2 minutes. Then, use a centrifuge at a speed of 8000 rpm Centrifuge for 12 minutes to make a probiotic slime, then add 1mL of compound freeze-drying protective agent per gram of probiotic slime to the probiotic slime, mix evenly with a vortex mixer, pre-freeze at -30°C for 18 hours, and then transfer to the freezer Freeze-drying in a dryer for 24 hours to obtain freeze-dried probiotic powder;
[0033] The compound lyoprotectant consists of 50% skimmed milk powder, 8% trehalose, 1% disodium hydrogen phosphate, 1% lactose and 40% phosphate buffer by mass percen...
Embodiment 3
[0037] 1) First, the probiotic Lactobacillus acidophilus was activated three times with MRS liquid medium at 37°C to obtain activated Lactobacillus acidophilus, and then Bifidobacterium infantis was added to the MRS liquid medium by adding 0.05% of the medium volume The cysteine hydrochloride was activated three times with MRS liquid medium under the culture condition of 37°C, and transferred each time with 5% inoculum size. The first two times were cultured at 37°C for 20 hours, and the third time Cultivate at 37°C for 18 hours, then perform microscopic examination and measure OD value, and then activate for the third time to obtain activated Bifidobacterium infantis after confirming that there is no miscellaneous bacteria. Take the activated probiotics Lactobacillus acidophilus and Bifidobacterium infantis Insert 10L of MRS medium (5g of cysteine hydrochloride in MRS medium) into fermenter with 5% inoculum and cultivate for 18h. Stir once every 4h during the cultivation p...
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