Method for quickly, simply and conveniently evaluating potential toxicity of molecules with planar structures

Abstract

The invention relates to a method for quickly, simply and conveniently evaluating potential toxicity of molecules with planar structures. A DNA (deoxyribonucleic acid) medicine intercalation theory is introduced into the method, an interaction relation of the molecules with the planar structures and DNA is researched by resonance light scattering technology, a corresponding binding saturation value of molecules with the planar structure and the DNA is calculated according to a measurement result of a resonance light scattering spectrum, and the corresponding potential toxicity of the molecules with the planar structures is further evaluated by means of adopting the binding saturation value of the molecules with the planar structures and the DNA as an evaluation index. By the aid of the method, the potential safety hazards of the planar molecules can be quickly, simply and conveniently evaluated, chemicals, medicines, healthcare food, food additives and food compositions with molecules with similarly planar structures can be quickly predicted and alerted, pharmacological and toxicological research for relevant pharmaceutical ingredients are supported technically, and the method effectively guides application of the molecules with the planar structures.

Description

Technical field [0001] The invention relates to a method for quickly and simply evaluating the potential toxicity of planar structure molecules. Background technique [0002] In 1961, Lerman proved that acridine is embedded in the base of DNA and proposed the DNA intercalator model for the first time. Planar aromatic fused ring structure molecules can be inserted into DNA as DNA intercalants. Deoxyribonucleic acid (DNA), as a carrier of genetic information, contains the most abundant information of organic organisms and is the target of many molecules. When the molecule is inserted into the DNA, the DNA conformation changes, or it is covalently cross-linked with the DNA strand, or the DNA is cut to hinder its replication and transcription, affect gene expression, and thus display cytotoxicity. In 1993, Pasternack et al. used a common fluorescence spectrophotometer to select appropriate excitation and emission passband widths, and used equal excitation and emission wavelengths to...

AI Technical Summary

Problems solved by technology

[0003] Many natural and synthetic molecules have a planar structure and are widely used in food additives, medicine, pesticides, and chemical industries, but have significant toxicity

At present, the research on the potential toxicity of related compounds is very weak, and the existing detection techniques are usually cumbersome and time-consuming, such as the in vitro test of sister chromatid exchange in mammalian animal cells, the micronucleus test in mammalian erythrocytes, and the chromosomal aberration in mammalian cells in vitro Tests, natural killer cell activity tests, etc., require complex detection equipment, cumbersome operations or take a long time

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