Atrac tylodes macrocephala koidz conservation method and re-cultivating method

A preservation method, Atractylodes macrocephala technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of genetic variation, labor and material resources, etc., achieve low cost, easy operation, and avoid germplasm mutation or destruction. Effect

Active Publication Date: 2012-11-07
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in tissue subculture, multiple subcultures may also lead to geneti

Method used

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  • Atrac tylodes macrocephala koidz conservation method and re-cultivating method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, the cryopreservation of Atractylodes Rhizome

[0041] 1. Reagent formulations

[0042] Pre-culture medium: MS liquid medium containing 0.3mol / L sucrose.

[0043] Pretreatment solution: MS liquid medium containing 2.0mol / L glycerol and 0.4mol / L sucrose.

[0044]Vitrification protectant: 3.26mol glycerol, 2.42mol ethylene glycol, 1.9mol dimethyl sulfoxide and 0.4mol sucrose were thoroughly mixed with MS liquid medium to 1L.

[0045] 2. Ultra-low temperature preservation of Atractylodes macrocephala

[0046] Take 2-month-old Atractylodes macrocephala tissue-cultured seedlings, peel off the outer leaves layer by layer until the remaining 1-2 leaf primordium surrounds the shoot apex meristem, and cut out 1.5-2mm shoot tips (a total of 100 shoot tips were obtained), Soak in the pre-culture solution for 3 days in the dark at 25°C, then soak in the pretreatment solution for 30 minutes with shaking at 25°C (normal light), then soak in the vitrification protectan...

Embodiment 2

[0055] Embodiment 2, the cryopreservation of Atractylodes macrocephala

[0056] 1. Reagent formulations

[0057] All the same as Step 1 of Example 1.

[0058] 2. Ultra-low temperature preservation of Atractylodes macrocephala

[0059] The time of dark cultivation at 25° C. in the pre-culture solution is 1 day, 2 days, 3 days, 4 days or 5 days respectively, and the others are the same as step 2 of Example 1.

[0060] 3. Statistics on recultivation, survival rate and regeneration rate

[0061] Same as Step 3 of Example 1.

[0062] The experiments were repeated three times, and the results were averaged.

[0063] When cultured in the dark at 25°C for 1 day in the pre-culture solution, the average plant survival rate of the three experiments was 69.8%. The average plant regeneration rate of the three experiments was 54.9%.

[0064] When cultured in the dark at 25°C for 2 days in the pre-culture solution, the average plant survival rate of the three experiments was 66.0%. Th...

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Abstract

The invention discloses an Atrac tylodes macrocephala koidz conservation method and re-cultivating method. The conservation method comprises the following steps: (1) taking stem tips of Atrac tylodes macrocephala koidz tissue culture seedlings, and placing the stem tips in a pre-culture solution which is an MS (Murashige and Skoog) fluid nutrient medium containing 0.3 mol/L cane sugar for pre-culture; (2) taking the stem passing through from the step (1) and pre-processing the stem tips in a pre-processing solution which is an MS fluid mutrient medium containing both 2.0 mol/L glycerol and 0.4 mol/L cane sugar; (3) taking the stem tips passing through the step (2) and processing the stem tips in a cryoprotective agent which is prepared in the way that 3.26 mol glycerol, 2.42 mol ethylene glycol, 1.9 mol dimethyl sulfoxide, 0.4 mol cane sugar and the MS fluid nutrient medium are uniformly mixed to 1 L; and (4) putting the stem tips passing through the step (3) into liquid nitrogen for conservation. The Atrac tylodes macrocephala koidz conservation method is low in cost, simple and convenient in operation and high in plant regeneration rate, and is an effective way in vitro conservation of Atrac tylodes macrocephala koidz germplasm resources.

Description

technical field [0001] The invention relates to a preservation method and a recultivation method of Atractylodes macrocephala. Background technique [0002] Atractylodes macrocephala (Atrac tylodes macrocephala Koidz.) is a plant of the Atractylodes genus in the Compositae family. Its rhizome is used as medicine and is an important medicinal plant. Due to the high market demand, long-term human excavation and environmental damage, the wild resources of Atractylodes macrocephala are scarce. At present, artificial cultivation is mainly used to ensure that the resources meet the market demand. Introduced and cultivated in most places in my country, Yuqian in Zhejiang and southern Anhui mountainous areas are used as authentic places. [0003] At present, Atractylodes macrocephala seeds or tuberous rhizomes are often used for preservation and propagation in the preservation garden or seed propagation garden through field planting. The use of seeds for propagation is prone to va...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张金梅陈晓玲卢新雄辛霞
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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