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Method for identifying different transgenic rice

A technology for transgenic rice and rice seeds, applied in measuring devices, material analysis through optical means, instruments, etc., can solve the problems of time-consuming, laborious, high cost, complicated operation, etc., and achieve high identification accuracy, reliable results, and simple operation Effect

Inactive Publication Date: 2012-11-07
CHINA JILIANG UNIV
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Problems solved by technology

Its identification uses the naked eye to directly observe the turbidity of the precipitate in the reaction tube or judge whether it is amplified by adding SYBR Green color change; this method involves specific primers and DNA amplification, which is costly, complicated to operate, time-consuming and laborious

Method used

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  • Method for identifying different transgenic rice

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Embodiment 1

[0027] 1. Build a model

[0028] (1) Select 40 trans-translational controlled tumor protein (TCTP) gene rice seeds and 40 trans-Osmi166 gene rice seeds as rice seed samples, and use Nicolet Nexus870 (Thermo Corporation USA) Fourier transform near-infrared spectrometer to test the rice seed samples The emission wave number ranges from 4000 to 10000cm -1 For the near-infrared spectrum of the spectral section, the diffuse reflectance spectrum information of all rice seed samples was collected using OMNIC 6.0 software; the number of scans of the near-infrared spectrometer was set to 32, and the resolution was 4cm -1 . During collection, the room temperature was controlled at about 25°C, and the humidity was kept stable.

[0029] The methods for obtaining the above TCTP-transgenic rice and Osmi166-transgenic rice are as follows: the transgenic materials use mature rice embryos as explants to induce and cultivate callus, select embryogenic callus as the transformation recipient, a...

Embodiment 2

[0050] 1. Build a model

[0051] (1) With embodiment 1.

[0052] (2) Use the standard normal transformation method to preprocess the diffuse reflectance spectrum information of all rice seed samples respectively to obtain the preprocessed spectrogram; use the principal component analysis method in the Unscrambler software to extract the wavenumber range after each pretreatment 4000~8000cm -1 For the spectral feature information of the spectral segment, the number of principal components is selected as 5, and the scores of each principal component are obtained (as shown in Table 4);

[0053] (3) Take the principal component scores corresponding to the spectral information of all rice seed samples as input, and use the transgenic type setting value corresponding to the rice seed sample as output to establish the PLS-DA model; set the transgenic type setting value corresponding to the rice seed sample As follows: the transfection of the TCTP gene was set to -1, and the transpos...

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Abstract

The invention discloses a method for identifying different transgenic rice. The method comprises the following steps of (1) emitting near infrared spectrums to rice seed samples, and collecting diffuse reflection spectral information of all the rice seed samples; (2) respectively preprocessing diffuse reflection spectral information of all the rice seed samples, extracting spectral characteristic information of a characteristic spectrum section subjected to the preprocessing through a principal component analysis method, and selecting principal components to obtain scores of the principal components; (3) establishing a model with the scores of the principal components corresponding to the spectral information of all the rice seed samples serving as input and transgenic type set values corresponding to the rice seed samples as output; and (4) obtaining scores of the principal components of spectrums of rice seeds to be measured, and putting the scores of the principal components into the model obtained in the step (3) to obtain transgenic types of the rice seeds. Trans-protein-gene rice and trans-controlling-gene rice are utilized in the method. The method is simple in operation, high in identification accuracy degree and capable of achieving fast and nondestructive identification of different transgenic rice.

Description

technical field [0001] The invention belongs to the detection field of transgenic plants, in particular to a method for identifying different transgenic rices. Background technique [0002] Transgenic technology refers to the introduction of exogenous genes into the genomes of other organisms through biological, physical or chemical means to obtain genetically improved bodies with stable inheritance and expression of exogenous genes. [0003] Rice is the largest grain crop in my country, and its annual output accounts for about 38% of the total grain output. Its production is related to the food security of the country. Transgenic technology breaks through the limitations of traditional rice breeding and provides a new way to ensure my country's food security. With the rapid development of rice genetic engineering technology, the research results of genetically modified rice are remarkable. In the past 20 years, a variety of transgenic rice has been successfully developed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35G01N21/3563G01N21/359
Inventor 朱诚张龙丁艳菲王珊珊
Owner CHINA JILIANG UNIV
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