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Method for extracting ovotransferrin from egg white

A kind of technology of ovotransferrin, egg white

Inactive Publication Date: 2012-11-28
HUAZHONG AGRI UNIV
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  • Description
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Problems solved by technology

[0004] The object of the present invention is to provide a kind of method that extracts ovotransferrin from egg white, the present invention is aimed at the deficiencies in the prior art, adopts the technical scheme that ethanol precipitation method combines with Q Sepharose FF anion exchange chromatography, and Appropriate process parameters are determined, which overcomes the problems of complex operation, small processing capacity, low product purity and yield in the prior art

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  • Method for extracting ovotransferrin from egg white
  • Method for extracting ovotransferrin from egg white
  • Method for extracting ovotransferrin from egg white

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Embodiment 1

[0021] A method for extracting ovotransferrin from egg white, comprising the following steps:

[0022] 1. Egg white pretreatment:

[0023] (1) Beat fresh eggs, use an egg yolk separator to separate the egg white and egg yolk, and collect the egg white;

[0024] (2) Take 1000mL egg white, add an equal volume of distilled water to the egg white while stirring, stir and mix evenly, and obtain the egg white dilution;

[0025] (3) Add 3~4g sodium bicarbonate to the egg white dilution while stirring, so that the molar concentration of sodium bicarbonate is about 20mM, and add about 6~9g sodium chloride at the same time, so that the molar concentration of sodium chloride is about 50mM , stir well, then add 40mL of 0.01mol / L FeCl 3 solution, fully stirred to fully saturate the ovotransferrin in the egg white with iron.

[0026] 2. Extract ovotransferrin:

[0027] Add absolute ethanol to make the final concentration of ethanol 40%, stir for 1 min, at this time, a precipitate occurs...

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Abstract

The invention discloses a method for extracting ovotransferrin from egg white, and the method comprises the following steps of: saturating an egg white diluent and iron; and combining ethanol precipitation and QSepharoseFF anion exchange chromatography. As transferrin saturation is carried out on the ovotransferrin in the egg white, the stability of the ovotransferrin is improved, the ovotransferrin can keep a natural structure in an ethanol solution with a certain concentration, and further the ovotransferrin can be extracted by using ethanol. The method for extracting the ovotransferrin from the egg white is simple in process flow and short in extraction cycle, and the obtained ovotransferrin product is high in purity and good in recovery rate.

Description

technical field [0001] The invention relates to a protein extraction method, in particular to a method for extracting ovotransferrin from egg white. Background technique [0002] Ovotransferrin in egg white is similar to lactoferrin in mammalian breast milk. It plays an important role in improving immune function, resisting bacterial and viral infections, and maintaining normal growth and development of the organism. In particular, its high affinity with iron ions makes it an important task of transporting iron in organisms, and plays a vital role in the absorption of iron in mammals. [0003] At present, there are many ways to separate and prepare ovotransferrin, but all of them have some shortcomings. In the early stage, the salting out method or organic solvent precipitation method was mainly used. The purity of ovotransferrin obtained was not high, and the natural structure and activity of the protein had been destroyed after high salt and organic solvent treatment. At...

Claims

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Application Information

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IPC IPC(8): C07K14/79C07K1/36C07K1/30C07K1/18
Inventor 马美湖任国栋耿放袁小军邱宁单媛媛
Owner HUAZHONG AGRI UNIV
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