Method for preparing composite biological agent by double-bacterium co-culture
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A composite biological bacteria, co-cultivation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of saving production costs, improving production efficiency, and enhancing physiological activity
Inactive Publication Date: 2012-12-12
SHANXI CHANGXIN BIOLOGICAL AGRI TECH
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example 1
[0023] Slant strains: Bacillus megaterium ①, nitrogen-fixing bacteria ② test tube slant, cultured at 25-32°C for 24-72h.
[0024] Strain suspension: Pour the strain ①② on the inclined surface of the test tube, respectively, into sterile water, scrape the bacterial lawn, and prepare the bacterial suspension.
[0025] Strain mixing: Mix the bacterial suspension of strain ①② in proportion.
[0026] Liquid culture medium: the medium used for the liquid culture of bacteria has the following components and weight ratio:
[0027] Soybean hydrolyzate 10~30g peptone 4~10g sucrose 16~24gKH 2 PO 4 3~8g MgSO 4 1~6g water 1L.
[0028] Sterilization: 0.12-0.15Mpa, 121-125°C, steam sterilization for 30 minutes.
[0029] Inoculation: Inject the strain suspension into the sterilized medium.
[0030] Cultivation: 25-32°C, tank pressure 0.04-0.05Mpa, culture for 36-72h.
example 2
[0032] Incline strains: lactic acid bacteria ③, Bacillus ④ test tube slant at 25-38 ℃ constant temperature culture, 24-72h.
[0033] Strain suspension: Pour the strain ③④ on the inclined surface of the test tube into sterile water, scrape the bacterial lawn, and prepare the bacterial suspension.
[0034] Strain mixing: Mix the bacterial suspension of strain ③④ in proportion.
[0035] Liquid medium: sucrose 10-30g peptone 4-10g yeast extract 5-10g KH 2 PO 4 5~10g MgSO 4 1~6g water 1L
[0036] Sterilization: 0.12-0.15Mpa, 121-125°C, steam sterilization for 30 minutes.
[0037] Inoculation: Inject the strain suspension into the sterilized medium.
[0038] Cultivation: 25-32°C, culture 2-7d.
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Abstract
The invention discloses a method for preparing a composite biological agent by double-bacterium co-culture. The method is characterized in that two microbes with different functionalities are co-cultured in a same fermentation apparatus. According to the invention, with life activities between the two microbes, and produced substances or a formed environment, the microbes are interdependent and are mutually benefited. At a same time, a preparation containing products of two microbes is obtained. According to physiological characteristics of strains, two microbes with different growth speeds and different growth periods are adopted, wherein the microbes can perform nutrition complementation, and can live in a same environment. With a co-culturing process, a product containing two functional microbes and metabolite thereof is obtained. Compared with independent culturing, the method provided by the invention is advantaged in improved production efficiency, reduced production cost, and substantially improved application effect. With the product, soil water-soluble phosphorus is increased, and soil nitrogen fixation capacity is improved.
Description
technical field [0001] The invention specifically relates to a method for preparing a compound biological agent by co-cultivation of two bacteria, which belongs to the field of biological fermentation, and uses two different functional microorganisms to co-cultivate in the same fermenter to finally obtain a preparation containing two products of microorganisms. Background technique [0002] Composite microbial preparations are considered to be products with complex functions of microorganisms. The significance of double-bacteria co-cultivation also lies in the fact that the two are interdependent and benefit from each other with each other's life activities, substances produced or environment created. Such products, represented by microecological compound preparations, are widely used in medicine, health care, agriculture, animal husbandry and other fields. The combination of double bacteria and the conditions of co-cultivation are always the technical key to the quality o...
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