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Method for extracting L-lactic acid produced in corn soaking process

A corn soaking and corn technology, applied in the field of separation engineering

Active Publication Date: 2014-05-28
JILIN COFCO BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the existing L-lactic acid extraction process, there is no report about the extraction of L-lactic acid produced by fermentation in the corn soaking process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Preparation of water for soaking corn: mix the following substances: glucose 20 g, molasses 10 g, peptone 2 g, K 2 HPO 4 ·3H 2 O 1 g, (NH 4 ) 2 SO 4 1 g, ammonium citrate 0.2 g, MgSO 4 ·7H 2 O 0.2 g, MnSO 4 ·H 2 O 0.01 g, FeSO 4 ·7H 2 O 0.01 g, biotin 6×10 -4 g, V B1 4×10 -4 g, into 1 L of water, CaCO 3 10 g, sterilized separately, maintained at 150°C for 2 hours, and then added to the water;

[0020] (2) Lactobacillus casei culture, seed medium composition: glucose 20g / L, peptone 5 g / L, yeast powder 5 g / L, K 2 HPO 4 ·3H 2 O 1 g / L, (NH 4 ) 2 SO 4 1 g / L, MgSO 4 ·7H 2 O 0.2 g / L, MnSO 4 ·H 2 O 0.01 g / L, FeSO 4 ·7H 2 O 0.01 g / L, pH 6.5, cultivated according to conventional methods to obtain Lactobacillus casei seed liquid;

[0021] (3) In a 500 mL Erlenmeyer flask, put 250 g of corn, 200 mL of water for soaking the corn, inoculate 20 mL of Lactobacillus casei seed solution, and pass through SO 2 , so that sulfite SO 2 The final concentr...

Embodiment 2

[0025] (1) Preparation of water for soaking corn: mix the following substances: glucose 20 g, molasses 10 g, peptone 2 g, K 2 HPO 4 ·3H 2 O 1 g, (NH 4 ) 2 SO 4 1 g, ammonium citrate 0.2 g, MgSO 4 ·7H 2 O 0.2 g, MnSO 4 ·H 2 O 0.01 g, FeSO 4 ·7H 2 O 0.01 g, biotin 6×10 -4 g, V B1 4×10 -4 g, into 1 L of water, CaCO 3 10 g, sterilized separately, maintained at 150°C for 2 hours, and then added to the water;

[0026](2) Lactobacillus casei culture, seed medium composition: glucose 20g / L, peptone 5 g / L, yeast powder 5 g / L, K 2 HPO 4 ·3H 2 O 1 g / L, (NH 4 ) 2 SO 4 1 g / L, MgSO 4 ·7H 2 O 0.2 g / L, MnSO 4 ·H 2 O 0.01 g / L, FeSO 4 ·7H 2 O 0.01 g / L, pH 6.5, cultivated according to conventional methods to obtain Lactobacillus casei seed liquid;

[0027] (3) Corn soaking, in a 100 L stainless steel tank, put 50 kg of corn, 40 L of water for soaking corn, inoculate 4 L of Lactobacillus casei seed solution, and pass through SO 2 , so that sulfite SO 2 The final ...

Embodiment 3

[0031] (1) Preparation of water for soaking corn: mix the following substances: glucose 20 g, molasses 10 g, peptone 2 g, K 2 HPO 4 ·3H 2 O 1 g, (NH 4 ) 2 SO 4 1 g, ammonium citrate 0.2 g, MgSO 4 ·7H 2 O 0.2 g, MnSO 4 ·H 2 O 0.01 g, FeSO 4 ·7H 2 O 0.01 g, biotin 6×10 -4 g, V B1 4×10 -4 g, into 1 L of water, CaCO 3 10 g, sterilized separately, maintained at 150°C for 2 hours, and then added to the water;

[0032] (2) Lactobacillus casei culture, seed medium composition: glucose 20g / L, peptone 5 g / L, yeast powder 5 g / L, K 2 HPO 4 ·3H 2 O 1 g / L, (NH 4 ) 2 SO 4 1 g / L, MgSO 4 ·7H 2 O 0.2 g / L, MnSO 4 ·H 2 O 0.01 g / L, FeSO 4 ·7H 2 O 0.01 g / L, pH 6.5, cultivated according to conventional methods to obtain Lactobacillus casei seed liquid;

[0033] (3) Corn soaking, in a 100 L stainless steel tank, put 50 kg of corn, 40 L of water for soaking corn, inoculate 4 L of Lactobacillus casei seed solution, and pass through SO 2 , so that sulfite SO 2 The final...

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PUM

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Abstract

The invention relates to a method for extracting L-lactic acid produced in the corn soaking process and belongs to the technical field of separation engineering. The method comprises the steps of corn soaking water preparation; lactobacillus casei culture; corn soaking; and soaking water separation and purification after soaking completion: firstly, centrifugation and active carbon decoloration are carried out; and ultrafiltration membrane separation and moving bed column loading are carried out, deionized water is used for carrying out elution, and the lactic acid extraction is completed. The method has the advantage that the problems that in the existing calcium lactate crystallization and acidolysis method, the extraction efficiency is low, the extracting agent of the extracting method is toxic, in addition, the L-lactic acid cannot be easily separated from the extracting agent, the static exchange capacity is low, and the like.

Description

technical field [0001] The invention belongs to the technical field of separation engineering, and specifically relates to an extraction method for L-lactic acid produced in corn soaking process. The method involves extracting and purifying L-lactic acid from corn soaking water by combining membrane separation method and moving bed chromatography separation method. Background technique [0002] Inject SO into water soaking corn 2 , to prevent the reproduction of other miscellaneous bacteria, and is conducive to the growth of lactic acid bacteria, so maintain a certain period of time, will accumulate a certain concentration of lactic acid. Soaking water contains sufficient nitrogen source and a large amount of carbon source, as well as about 20 g / L L-lactic acid, and the usual practice is to treat it as wastewater. [0003] Qian Dongping and others first separated the protein from the corn soaking water through the isoelectric point method, ultrafiltration method, and trichl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56C07C59/08C07C51/47C12R1/245
Inventor 佟毅王宏龄金贞花张国峰王国良赵雪松
Owner JILIN COFCO BIOCHEM