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Primer for diagnosing lung lesions caused by chrysotile and man-made mineral fiber by using RT-PCR method

A RT-PCR, mineral fiber technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., can solve the problems of unusable, short sequence, and lack of miRNA

Inactive Publication Date: 2012-12-19
ZHEJIANG ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of quantitative detection of miRNA, since mature miRNA does not have a poly(A) tail and its sequence is very short, conventional oligo d(T) or random primers cannot be used for its reverse transcription reaction

Method used

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  • Primer for diagnosing lung lesions caused by chrysotile and man-made mineral fiber by using RT-PCR method
  • Primer for diagnosing lung lesions caused by chrysotile and man-made mineral fiber by using RT-PCR method
  • Primer for diagnosing lung lesions caused by chrysotile and man-made mineral fiber by using RT-PCR method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] 1. Dust preparation

[0037] Grind glass fiber, rock wool, refractory ceramic fiber factory materials and chrysotile samples into powder in an agate mortar with water, bake at 180°C for 2 hours, and continue grinding to a very fine powder. After drying the dust of the standard product at 180°C for 2 hours, weigh it, and grind it into a very fine powder in an agate mortar. The degree of dispersion is shown in the table below:

[0038]

[0039] 2. Establish dust-infected rat model

[0040] Dissolve the ground powder in physiological saline to prepare a suspension (10 mg / ml for chrysotile suspension and 20 mg / ml for artificial mineral fiber suspension). Autoclave until ready to use.

[0041] 90 two-week-old male SD rats were randomly divided into 9 groups, including blank control group (BL), normal saline group (NS), chrysotile group (AS), rock wool standard group (RWS), refractory ceramic fiber standard group Group (RFS), glass fiber standard group (GWS), rock wool...

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Abstract

The invention relates to a primer for diagnosing lung lesions caused by chrysotile and man-made mineral fiber by using an RT-PCR method. The primer comprises a primer pair 1, a primer pair 2 and a primer pair 3 which are respectively used for amplifying miR-29a, miR-29b and miR-29c, and a base sequence is showed as SEQ ID NO.4-9. An miRNA amplification primer is designed on the basis of rat lung tissue pathological mechanism expressions by building a rat dust dying model, expression situations of miR-29 family members in dust dying rat lung tissues are detected, and an effective tool of early diagnosis and screening of health hazards caused by exposure of the chrysotile and the man-made mineral fiber is provided.

Description

technical field [0001] The invention relates to a primer, in particular to a primer for diagnosing lung lesions caused by chrysotile and artificial mineral fibers. Background technique [0002] Asbestos is a natural fibrous silicate mineral. Due to its excellent properties such as heat resistance, heat preservation, wear resistance, electrical insulation and chemical corrosion resistance, it has been widely used in asbestos cement, textiles, and insulating friction materials for many years. manufacture. There are many types of asbestos, which can be divided into two categories: amphibole asbestos (including crocidolite, amosite, etc.) and serpentine asbestos (chrysotile) according to different chemical components. Due to its fibrous properties, asbestos is inhaled by the human body, adheres to and deposits in the lungs, and can cause pulmonary fibrosis, mesothelioma, and pleural plaques. Among the asbestos family, the most harmful is crocidolite, which is highly carcinogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 张幸武为肖芸朱丽瑾冯玲芳张敏
Owner ZHEJIANG ACAD OF MEDICAL SCI
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