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Valosin fragment, and preparation method and application thereof

An application and amino acid technology, applied in the direction of peptide sources, pharmaceutical formulations, animal/human peptides, etc., can solve the problems of insulin resistance, poor effect, etc., and achieve obvious effects.

Active Publication Date: 2014-01-15
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main drugs for the treatment of diabetes are drugs that promote insulin secretion and direct insulin supplementation, but these drugs are either ineffective or cause insulin resistance

Method used

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  • Valosin fragment, and preparation method and application thereof
  • Valosin fragment, and preparation method and application thereof
  • Valosin fragment, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In this embodiment, the following preparation steps are adopted:

[0022] (1) Take 30 kg of fresh pig intestines, wash the pig intestines and immerse them in boiling water for 4 minutes. After soaking, take them out, freeze them and chop them, and then treat the intestines with 0.5mol / L acetic acid at 5°C. After leaching, filter to obtain the leach solution, then use 0.2mol / L hydrochloric acid to elute the leachate in the leach solution and filter to obtain about 30 grams of heat-resistant intestinal polypeptide, and the yield of heat-resistant intestinal polypeptide is about the wet weight of pig intestine 0.01% of.

[0023] (2) Dissolve 30 grams of heat-resistant intestinal polypeptide in 0.24L of water, add 1.2ml of antioxidant thiodiglycol and 1.08L of isopropanol solution, let stand at room temperature for 2 hours, centrifuge to remove the precipitate, and then add 1.32L The temperature is -20°C isopropanol solution and stand at -20°C for 24 hours, then remove the...

Embodiment 2

[0034] In this embodiment, the following preparation steps are adopted:

[0035] (1) Take 20 kg of fresh pig intestines, wash the pig intestines and immerse them in boiling water for 6 minutes. After soaking, take them out, freeze them and chop them, and then treat the intestines with 0.5mol / L acetic acid at 8°C. After leaching, filter to obtain the leach solution, then use 0.2mol / L hydrochloric acid to elute the extract in the leach solution and filter to obtain about 19 grams of heat-resistant intestinal polypeptide, and the yield of heat-resistant intestinal polypeptide is about the wet weight of pig intestine 0.01% of.

[0036] (2) Dissolve 19 grams of heat-resistant intestinal polypeptide in 0.15 L of water, add 1 ml of antioxidant thiodiglycol and 0.7 L of isopropanol solution, let stand at room temperature for 2 hours, centrifuge to remove the precipitate, and then add 0.89 L Isopropanol solution with a temperature of -23°C and stand at -20°C for 24 hours, then remove ...

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Abstract

The invention provides a polypeptide, a preparation method and an application thereof. The polypeptide is a truncated valosin fragment and is composed of 3-25 sited amino acid residues with the amino acid sequence of YPVEHPDKFLKFGMTPSKGVLFY. The preparation method for the polypeptide comprises the steps of taking heat-resistant intestinal polypeptide out from pig intestinal canal; extracting crude peptide form the heat-resistant intestinal polypeptide; dissolving the crude peptide in an acetic acid solution to obtain a peptide solution; passing the peptide solution through a column to carry out column chromatography separation to obtain a separated material; selecting a part having the function of stimulating pancreas to excrete insulin from the separated material; freeze-drying to obtain a crude extract; and purifying the crude extract to obtain the polypeptide. The invention also provides an application of the polypeptide and pharmaceutically acceptable salt thereof. The polypeptide and pharmaceutically acceptable salt thereof can be used for producing drugs capable of stimulating the pancreas to excrete insulin.

Description

technical field [0001] The invention relates to a truncated fragment of valoropeptide, and the polypeptide fragment has the effect of stimulating insulin secretion, and the invention also provides a preparation method of the above polypeptide fragment. Background technique [0002] Valtyropeptide is a twenty-five-peptide, which is composed of twenty-five amino acids connected by peptide bonds. It was originally isolated from the small intestine of pigs and is currently mainly used in the treatment of tumors in the digestive system. [0003] Diabetes is a disease in which excess glucose tends to accumulate in the blood. It is nicknamed "Silent Killer" abroad, especially "adult diabetes". The prevalence rate of middle-aged people over the age of 40 is particularly high. In Japan, the incidence rate of the population over the age of 40 accounts for 10%, that is, there is one diabetic patient in every ten people. Once suffering from "diabetes", life expectancy will be reduced b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C07K1/16C07K1/14A61K38/17A61P3/10
Inventor 李臣鸿汪俊汉陈正望
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES