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Body immune function-enhancing cell biological drug, its preparation method and application

A technology of immune cells and body immunity, applied in the field of new biological drugs, can solve the problems of insufficient CD8T cell expansion, reduced anti-tumor activity of effector cells, and inability to produce large-scale drugs, achieving high safety, good effects, The effect of improving basal immune function

Active Publication Date: 2013-01-02
SHANGHAI XINGHUA BIOMEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] In view of the lack of precursors of CD8 T cells in the prior art, the expansion speed of CD8 T cells is not fast enough, the cytokines that stimulate the growth of CD8 T cells are not well understood, and with the extension of culture and expansion time, the anti-tumor effect of effector cells The activity is reduced, and allogeneic lymphocytes cause immune rejection, which cannot be used as a large-scale production of medicines. The present invention provides a method for preparing medicines that improve the immune function of the body, which overcomes the above-mentioned various problems

Method used

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  • Body immune function-enhancing cell biological drug, its preparation method and application
  • Body immune function-enhancing cell biological drug, its preparation method and application
  • Body immune function-enhancing cell biological drug, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Allogeneic mononuclear cells are co-cultured with specific antigens in a liquid medium, which includes concanavalin, liver cancer cells (antigens), exogenous superantigens, lymphokines, and immune adjuvants, among which knife The amount of soy bean in the medium is 1 μg / L; the amount of exogenous superantigen (Pseudomonas aeruginosa toxin) in the medium is 5 μg / L; the amount of lymphokine (interleukin II) in the medium is 1000u / L ; The dosage of immune adjuvant (5% Tween-80) is 0.1mL / L.

[0067] The ratio of mononuclear cells to specific antigen (hepatoma cells) is 5:1 (cell number ratio).

[0068] Mononuclear cells were co-cultured with specific antigens for 30 days to obtain specific immune cell cultures against the corresponding antigens.

[0069] Cells with an immune response, mainly cytotoxic T lymphocytes, are isolated from the culture.

Embodiment 2

[0071] Allogeneic mononuclear cells are co-cultured with specific antigens in liquid media, including phytohemagglutinin, allogeneic lung cancer cells (antigens), exogenous superantigens, lymphokines, and immune adjuvants , in which, the dosage of phytohemagglutinin (PHA) in the culture medium is 5 μg / L; the dosage of exogenous superantigen (Pseudomonas aeruginosa toxin) in the culture medium is 1 μg / L; the dosage of lymphokine (interferon) in the culture medium The dosage of medium is 1000u / L; the dosage of immune adjuvant (5% Tween-80) is 0.1mL / L. At the same time, autologous dendritic cells of antigen-presenting cells were added.

[0072] The ratio of mononuclear cells to specific antigen (allogeneic lung cancer cells) is 100:1 (cell number ratio).

[0073] The ratio of mononuclear cells to antigen-presenting cells (autologous dendritic cells) is 50:1 (cell number ratio).

[0074] Mononuclear cells were co-cultured with specific antigens for 90 days to obtain specific imm...

Embodiment 3

[0077] Allogeneic mononuclear cells (genetically modified T lymphocytes) are co-cultured with specific antigens in a liquid medium, which includes phytohemagglutinin, HIV virus (antigen), exogenous superantigen, cell Factors, immune adjuvants. Among them, the amount of phytohemagglutinin in the medium is 10 μg / L; the amount of HIV virus in the medium is 10 μg / L; 2 copies / L; the amount of exogenous superantigen (Pseudomonas aeruginosa toxin) in the medium is 5 μg / L; the amount of lymphokine (GM-CSF) in the medium is 500u / L; immune adjuvant (5% Tween -80) The dosage is 0.1mL / L.

[0078] The ratio of mononuclear cells to antigen-specific HIV virus is 100:1 (number of cells: number of copies of virus).

[0079] Mononuclear cells were co-cultured with specific antigens for 10 days to obtain specific immune cell cultures against corresponding antigens.

[0080] Cells with an immune response, mainly cytotoxic T lymphocytes, are isolated from the culture.

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Abstract

The invention provides a preparation method of a body immune function-enhancing biological drug. The method includes: step 1. subjecting an immune cell and a specific antigen to co-culture in a liquid cell medium, which comprises a cell mitogen, an antigen, a super antigen, a cell factor, and an immune adjuvant, etc., thus obtaining a specific immune cell culture resisting the corresponding antigen; and step 2. separating a cell with an immune response from the culture obtained in step 1. The biological preparation and drug prepared in the invention have good safety, high activity, and can effectively improve the body immune function, thus being able to be used in treatment and prevention of malignant tumors, virus infections (such as acquired immune deficiency syndrome and hepatitis) and other immune function related diseases.

Description

technical field [0001] The present invention relates to a novel biomedicine, its preparation method and application, in particular to the preparation of a cell-based biopharmaceutical that can improve the immune function of the body and can be applied to the treatment and prevention of malignant tumors and viral infections (such as AIDS and hepatitis) method. Background technique [0002] The immune system is a defensive system for the body to protect itself. It can accurately identify self and non-self substances to maintain the relative stability of the body. At the same time, it can also receive, transmit, expand, store and remember immune-related information. Enhanced and diminished responses occur and their reactivity is constantly adjusted. The basic functions of the immune system are: immune defense, immune stabilization and immune surveillance. [0003] The immune system is composed of immune organs, immune cells and immune molecules. The immune response can be di...

Claims

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Application Information

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IPC IPC(8): A61K39/00C12N5/078C12N5/0783C12N5/0786A61P37/02A61P37/04
Inventor 刘华
Owner SHANGHAI XINGHUA BIOMEDICAL TECH
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