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Genetically engineered bacteria for degrading phenol in petrochemical wastewater

A technology of genetically engineered bacteria and petrochemical wastewater, applied in the field of genetically engineered bacteria that degrade phenol, can solve the problem of low phenol degradation ability

Inactive Publication Date: 2013-01-02
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of low phenol degradation ability of wild strains isolated and identified from petrochemical wastewater, and to provide a genetically engineered bacterium that degrades phenol in petrochemical wastewater

Method used

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specific Embodiment approach 1

[0012] Specific embodiment 1: In this embodiment, the genetically engineered bacterium for degrading phenol in petrochemical wastewater is Bacillus subtilis dqly-2, which is preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, and the preservation address is Chaoyang, Beijing No. 3, No. 1 Yard, Beichen West Road, District, the preservation date is December 20, 2011, and the preservation number is CGMCC No.5619.

[0013] In this embodiment, Bacillus subtilis (Bacillus subtilis) dqly-2 is a Gram-positive aerobic bacterium with a length of 1.0-1.2 μm and a width of 0.7-0.8 μm, endospores, spores, and the surface of free spores is colored Weak, with lateral flagella; irregular round colonies formed on the agar medium, the surface is dark, opaque, rough, wrinkled, milky white or brown.

[0014] The above-mentioned agar medium (1 L) is composed of 10 g of tryptone, 5 g of yeast extract, 10 g of NaCl, 12 g of agar and the rest of d...

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Abstract

The invention relates a strain of genetically engineered bacteria for degrading phenol in petrochemical wastewater, solving the problem that wild bacterial strains for isolating and identifying phenol in petrochemical wastewater has a low capacity of degrading phenol in the prior art. The genetically engineered bacteria strain is (Bacillus subtilis) dqly-2 which is preserved in China General Microbiological Culture Collection Center as on Dec 20, 2011 as the preservation number of CGMCC No.5619. According to the invention, phenol can be degraded to achieve a concentration of 0.3825mg / L in 96h by the (Bacillus subtilis) dqly-2 at the extreme, and the highest degradation rate of phenol can reach to 96.18%, and the wastewater treated by the (Bacillus subtilis) dqly-2 reaches the national first-degree wastewater discharge standard.

Description

technical field [0001] The invention relates to a genetically engineered bacterium for degrading phenol. Background technique [0002] Phenolic compounds are highly toxic and are one of the priority pollutants to be controlled in my country, as well as one of the 129 priority substances listed by the U.S. Environmental Protection Agency. Phenol-containing wastewater mainly comes from coking, gas, oil refining, and chemical and pharmaceutical production processes that use phenol or phenolic formaldehyde as raw materials. It has a wide range of sources, a large amount, pollutes water sources, poisons fish and shrimp, endangers crops, and seriously endangers human health. In the past decade, microorganisms that degrade phenol have received increasing attention. [0003] Some phenol-degrading bacteria have been isolated and identified from petrochemical wastewater in the current related research, including Bacillus thuringiensis and Pseudomonas, etc. However, most of the degrad...

Claims

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Application Information

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IPC IPC(8): C12N1/21C02F3/34C12R1/125C02F101/34
Inventor 刘宇峰杨庆丽姬妍茹董艳高媛石杰
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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