Breeding method of small strain in vitamin C two-step fermentation

A two-step fermentation method, vitamin technology, applied in biochemical equipment and methods, microorganisms, and treatment of microorganisms with electricity/wave energy, etc., can solve the problems that the excellent characteristics of the target strain are difficult to be fully expressed, and the difficulty of screening is increased. Achieve the effect of short cycle, high acid production and high conversion rate of sugar and acid

Inactive Publication Date: 2013-01-02
NINGXIA QIYUAN PHARMA
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Problems solved by technology

Because the small bacteria are small, if a single colony is not further value-added culture, just use the existing technology to collect a certain number of small bacteria after mutagenesis and mix them for overall inspection, because they are not screened one by one

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  • Breeding method of small strain in vitamin C two-step fermentation

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Embodiment Construction

[0014] The specific examples exemplified below are only for explaining the present invention, and do not constitute any limitation to the present invention.

[0015] 1 Breeding steps: separate the seed liquid in the logarithmic growth phase to the plate culture, collect a certain number of small bacteria for mutagenesis and spread them on the plate, overlap the large bacteria for plate culture, and select the mutated bacteria after the culture is over. The obtained single colonies of small bacteria were spread on the plate one by one, overlapped with the large bacteria for proliferation and culture, and then connected to the inclined surface one by one, overlapped with the large bacteria to cultivate the inclined surface, and shake the flask to screen the target strains. Statistical analysis of experimental data, preliminary screening and retention of seeds.

[0016] 2 Breeding screening process:

[0017] 2.1 Separation of large and small bacteria:

[0018] Take out the stor...

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Abstract

The invention relates to a breeding method of small strians in vitamin C two-step fermentation. According to the method, a seed liquid in a logarithmic growth phase is subjected to plate cultivation; small strains are selected out, mutagenized and coated on the plate; small strains are lapped with large strains for plate cultivation; and then the strains are coated on the plate and lapped with large strains for proliferation cultivation; the strains are connected to an inclined plane one by one, and lapped with large strains for slant cultivation; and flask shaking is carried out for screen target strains. According to the invention, mutagenized small single colony strains are screened by a proliferation cultivation method, so that strains with various characteristics can be reflected one by one; and non-target strains are washed out to successfully obtain the target strains, so as to improve the probability of obtaining target strains with good performance.

Description

technical field [0001] The invention relates to the technical fields of biology and medicine, in particular to a method for breeding small bacteria in a vitamin C two-step fermentation method. Background technique [0002] Vitamin C (Vitanun C, Vc) is an essential vitamin for human nutrition. It is not only used as an important medical product to treat various diseases, but also widely used in food, feed and cosmetics. With the increase of the application range of vitamin C, the market demand is increasing day by day, so that people continue to study and improve the production technology of VC. [0003] At present, the fermentation production of vitamin C is realized by a two-step method, that is, Bacillus (commonly known as large bacteria, mainly Bacillus megaterium, Bacillus cereus and Bacillus thuringiensis) and Gluconobacter oxidans (commonly known as small bacteria) mixed bacteria fermentation , the small bacteria are acid-producing bacteria, which can produce 2-keto-L...

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Application Information

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IPC IPC(8): C12N15/01C12N13/00
Inventor 李学兵郭惠芬陈荔杨玉芳蒋鸿
Owner NINGXIA QIYUAN PHARMA
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