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Reagent kit for detecting H7N1 avian influenza virus

An avian influenza virus, H7N1 technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problem of lack of simple and rapid detection methods, and achieve high sensitivity, low cost, and improved sensitivity.

Inactive Publication Date: 2013-01-09
天津朝海科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The H7N1 avian influenza virus has a great impact on human life, but there is currently no simple, fast, easy-to-use and low-cost detection method for the H7N1 avian influenza virus

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  • Reagent kit for detecting H7N1 avian influenza virus

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Embodiment Construction

[0022] The kit of the present invention includes 2 tubes of solutions, one tube is 50 μl of a nano-gold particle solution labeled with a molecular probe that can recognize the specific sequence of the H7N1 avian influenza virus, and the other tube is 250 μl of a solution that can amplify the DNA in the sample to be detected. H7N1 avian influenza virus DNA or a loop-mediated isothermal nucleic acid amplification system for amplifying H7N1 avian influenza virus RNA in a sample to be detected after a reverse transcription process.

[0023] The gold nanoparticle solution contains gold nanoparticles with a diameter of 20 nm at a concentration of 1.2 nM, and the surface of the gold nanoparticles is marked with thiol-modified DNA probes.

[0024] The components of the loop-mediated isothermal nucleic acid amplification system are as follows: 0.2 μM F3 and B3 primers, 1.6 μM FIP and BIP primers, 0.4 M betaine (Sigma–Aldrich, St. Louis, MO), 10 mM MgSO4, 1.4 mM dNTPs, 1× ThermoPol reac...

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Abstract

The invention relates to a reagent kit for detecting deoxyribonucleic acid (DNA) of a product by using gold nano-particles after the DNA of H7N1 avian influenza virus is amplified by means of a loop-mediated isothermal nucleic acid amplification technology and belongs to the technical field of biological detection. The reagent kit combines the loop-mediated isothermal nucleic acid amplification technology and a biological nano-technology, improves the sensitivity of biomolecule detection, is simple in operation, quick and accurate, does not need special instruments and devices, and can be widely applied to the detection of high-sensitivity H7N1avian influenza virus in the fields of household diagnosis, clinical diagnosis, communicable disease control, environmental monitoring, inspection and quarantine, biotechnologies and the like. The reagent kit comprises (1) the gold nano-particles marked with molecular probes capable of identifying specific sequences of the H7N1 avian influenza virus, and (2) a loop-mediated isothermal nucleic acid amplification system capable of amplifying the DNA of the H7N1 avian influenza virus in a sample to be detected or firstly performing reverse transcription and then amplifying ribonucleic acid (RNA) of the H7N1 avian influenza virus in the sample to be detected.

Description

technical field [0001] The invention relates to a kit for detecting H7N1 avian influenza virus DNA or RNA amplified by a loop-mediated isothermal nucleic acid amplification technology by using nano gold particles, and belongs to the technical field of biological detection. Background technique [0002] The high sensitivity, high specificity, simple and rapid detection of genes (DNA) and their transcription products (RNA) is very useful for the early diagnosis and treatment of diseases, as well as in the fields of health and epidemic prevention, environmental monitoring, inspection and quarantine, etc. Significance. In the past, more accurate and sensitive detection methods in this regard include fluorescent labeling, radioactive labeling and other methods. These methods require complex operations and special equipment, and their application range is very narrow. In 1993, Dr. Kary B. Mullis of PE Company invented the polymerase chain reaction (Polymerase Chain Reaction), ref...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68
Inventor 不公告发明人
Owner 天津朝海科技有限公司