Method for separating and extracting ovotransferrin

Abstract

The invention discloses a method for separating and extracting ovotransferrin, and particularly provides a simple separation and extraction method which has the advantage of high product purity and can implement industrial production. The method comprises the following steps: regulating the pH value of a mucin-free egg white solution to 7.5-9.0, and extracting with an ethanol precipitation method to obtain an ovotransferrin crude product; and purifying the ovotransferrin crude product by a two-step weakly-acidic cation exchange resin chromatography method, and dialyzing the purified ovotransferrin at 1-10 DEG C for desalination, thereby obtaining the high-purity ovotransferrin. The ovotransferrin is extracted and separated by an alcohol precipitation method combined with a weakly-acidic cation exchange method: the ovotransferrin is prepared into a compound under alkaline conditions, and subjected to the alcohol precipitation method to obtain the ovotransferrin crude product; and the ovotransferrin crude product is subjected to the two-step weakly-acidic cation exchange resin chromatography method. The product purity is high; the preparation scale and yield of the ovotransferrin are enhanced; and the method is simple, and is beneficial to implementing industrialized production.

Description

technical field [0001] The invention relates to the technical field of organic compound extraction, in particular to a method for separating and extracting ovotransferrin. Background technique [0002] Ovotransferrin, also known as paraovalbumin or ovaconalbumin, is a single-subunit glycoprotein containing 686 amino acid residues in poultry egg white, accounting for about 10-13% of egg white protein. It is 77-80ku, and its pI value is 6.0-6.7. Ovotransferrin has the ability to reversibly bind ferric ions and transfer iron. It also has antibacterial functions, antiviral functions, regulation of physiological functions, and inhibition of free radical generation. It is widely used in the pharmaceutical and food industries. application prospects. [0003] At present, the methods used to extract and prepare ovotransferrin at home and abroad include salt precipitation method, membrane separation technology method, anion-cation resin exchange method, immobilized metal chelation a...

AI Technical Summary

Problems solved by technology

These chromatographic separation methods provide a certain method for extracting and separating ovotransferrin to a certain extent, although some of these methods can make the purity of the product reach a higher level, the scale of its preparation is very small and is not suitable for industrial production. , especially methods using gel filtration chromatography, and; some prepared products with lower purity

Moreover, the preparation cost of these methods is high, especially the preparation medium used, it is difficult to expand into an industrial preparation method

In 2008, K.Y.Ko and D.U.Ahn used alkaline conditions (pH=9.0) with accompanying anions (HCO 3 - ) in the presence of ovotransferrin through HCO 3 - with Fe 3+ form a ternary complex, namely Fe 3+ 2 -HCO 3 - -Ovotransferrin complex, after the complex is formed, the entire protein molecular structure becomes tighter, closed, and stable, and its tolerance to ethanol precipitation is improved, so let ovotransferrin in alkaline conditions first The complex is generated under the following conditions, and then the ovotransferrin is extracted and separated by the alcohol analysis method. The preparation scale of this method has the possibility of being enlarged to an industrial scale, but the purity of the product is only 80%.