Cryptococcus gattii, and isolated culture method and application thereof

A technology for the isolation and cultivation of Cryptococcus gattii, applied in the biological field, can solve the problems of lack of epidemiology, virulence, diagnosis and treatment of Cryptococcus gattii

Inactive Publication Date: 2013-01-16
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is still a lack of research on the epidemiology

Method used

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  • Cryptococcus gattii, and isolated culture method and application thereof
  • Cryptococcus gattii, and isolated culture method and application thereof
  • Cryptococcus gattii, and isolated culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Cryptococcus gattii (Cryptococcus gattii) S8012

[0039] Namely, Cryptococcus neoformans var.gattii strain CGMCC No.6018, belonging to Basidiomycota, Tremellomycetes, Tremellales, Tremellaceae smut Genus (Filobasidiella).

[0040] The strain of the present invention is separated and detected from the cerebrospinal fluid of patients with cryptococcal meningitis by the Dermatology Department of Shanghai Changzheng Hospital. It was deposited in the General Microbiology Center (CGMCC) of the China Microbial Culture Collection Management Committee on April 16, 2012, with the deposit registration number CGMCCNO.6018.

[0041] 1. Morphological characteristics:

[0042] The cerebrospinal fluid ink staining shows the morphology of rod-shaped, needle-shaped, spindle-shaped and scoop-shaped cells of broad capsular membrane. Such as figure 1 Shown.

[0043] 2. Training characteristics:

[0044] Cultivate on Sabouraud medium at 30°C and start to grow the next day. The colonies ...

Embodiment 2

[0059] Example 2: Fungal susceptibility test

[0060] With reference to the M27-A3 method (Clinical Laboratory and Standards Institute. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. Approved standard M27-A3. Wayne, PA: National Committee for Clinical Laboratory Standards, 2008.), the Cryptococcus gordonii The drug sensitivity of amphotericin b, flucytosine, fluconazole, itraconazole and voriconazole was determined. The MIC values ​​are 0.25, 1, 4, 0.25 and 0.063μg / mL respectively.

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Abstract

The invention relates to the field of biological technologies, in particular to cryptococcus gattii, and an isolated culture method and application thereof. The invention aims to perform isolated culture and purification on a strain of cryptococcus gattii and provides the strain of cryptococcus gattii which was collected in China General Microbiological Culture Collection Center (CGMCC) in April 16, 2012 and has the collection number of CGMCC No.6018. The invention performs further research on the morphological characteristic and the culture characteristic of the strain and the utilization of carbon source. The invention further provides a method for performing isolated culture and purification on the strain and application thereof to screening of anti-fungal medicines.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a Cryptococcus gattii (Cryptococcus gattii) and its isolation and culture method and application. Background technique [0002] Cryptococcus gattii (Cryptococcus gattii) is a basidiomycete yeast with a capsule, which is mainly distributed in tropical and subtropical areas and often infects people with normal immunity. Molecular typing shows that it is composed of four main genotypes: VGI-VGIV. Among them, the VGII genotype caused an outbreak of cryptococcal meningitis on Vancouver Island, Canada. In my country, Cryptococcus neoformans is the main pathogen of cryptococcal meningitis. [0003] With the deepening of phylogenetic research, the Cryptococcus neoformans Gerte variety has now risen to the level of species, and is named Cryptococcus gareth (Kwon-Chung KJ, Boekhout T, Fell, JW, Diaz M. Proposal to conserve the name Cryptococcus gattii against C. hondurianus and C. basillisporus (B...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12Q1/18C12R1/645
Inventor 廖万清
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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