Method for identifying salt-resistant capability of cotton by using death rate of leaf lower epidermic cells

Abstract

The invention relates to a method for identifying salt-resistant capability of cotton by using death rate of leaf lower epidermic cells. The method comprises the steps of selecting leaves with identical thickness in a cotyledon stage of the cotton, and tearing the lower epidermis from the leaves gently; putting the prepared lower epidermis in a NaCl solution with a concentration of 3.5 M, and treating for 10-15 min; staining the epidermis treated by the salt solution for 5-10 min by using a 0.03-0.05% neutral red solution; immersing the intravitally stained epidermis in tap-water with a pH value a little higher than 7 for 3-5 min; observing death situation of the epidermic cells under an optical microscope; recording the deaths of the cells in the same area according to a principle that living cells are stained into cherry-red color while the dead cells cannot stained; and determining the salt-resistant capability of the cotton through the salt-resistant capability of leaf lower epidermic cells. The method can determine genotype salt-resistant capability rapidly and accurately, is labor-saving and time-saving time, is not influenced by seasons and environment, and increases identification efficiency.

Description

technical field [0001] The invention relates to a method for detecting salt tolerance of crops, in particular to a method for identifying the salt tolerance ability of cotton by using the death rate of epidermal cells under cotyledons. Background technique [0002] At present, there are different opinions on the identification methods of plant salt tolerance, which can be summarized as direct identification method and indirect identification method. Direct identification includes germination comparison method, morphological comparison method and yield comparison method. Indirect identification is to use physiological and biochemical analysis methods to study the material changes in the physiological metabolism process of plants under salt stress and conduct salt tolerance identification. The main indicators are: proline, plasma membrane permeability, chlorophyll, K+ / Na+, enzyme activity, etc. . In cotton salt tolerance identification, also due to the different test conditio...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for identifying cotton tolerance by using the death rate of lower epidermal cells of cotyledons to solve the shortcomings of the current cotton salt tolerance identification methods, such as inconsistency, lack of depth, long identification period in the field or in salt ponds, and great influence from the external environment. method of salt ability