Eucalyptus PGEF17 gene, and plant expression vector, host cell and application thereof

A plant expression vector and host cell technology, applied to plant genes and their application fields, can solve problems such as complex genetic diversity, and achieve the effects of increasing plant biomass, increasing biomass, and promoting plant vegetative growth.

Inactive Publication Date: 2013-01-23
普罗米绿色能源(深圳)有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Forest trees and other woody plants are rich in genetic resources and complex genetic diversity, with many genes with potential application value but not yet fully explored and effectively isolated

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Eucalyptus PGEF17 gene, and plant expression vector, host cell and application thereof
  • Eucalyptus PGEF17 gene, and plant expression vector, host cell and application thereof
  • Eucalyptus PGEF17 gene, and plant expression vector, host cell and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] The present invention will be described in more detail below through specific embodiments and with reference to the accompanying drawings. It should be understood that the examples described below are only for illustration rather than limitation of the present invention.

[0027] 1. Cloning and sequence analysis of PGEF17 gene

[0028] 1. Preparation of Eucalyptus cDNA

[0029] Choose fresh eucalyptus ( Eucalyptus ) leaves, such as Eucalyptus eucalyptus species DH32-29 ( Eucalyptus urophylla × grandis cv DH32-29) leaves were quickly frozen in liquid nitrogen, and then stored in a -80°C ultra-low temperature freezer (SANYO, MDF-382E) for later use.

[0030] Total RNA was extracted from Eucalyptus leaves according to the existing Cetyltrimethyl Ammonium Bromide (CTAB) extraction method (Chang et al., Plant Molecular Biology Reporter 11(2): 113-116 (1993)). Extracted RNA was treated with RNase-free DNase (Takara, Cat# D2270A) to remove contaminating genomic DNA.

[0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a eucalyptus PGEF17 gene. The complementary deoxyribonucleic acid (cDNA) sequence of the gene is characterized by having an DNA sequence which is shown as SEQIDNO:1 or encoding polypeptide which has the amino acid sequence shown as SEQIDNO:2. The invention also provides a host cell which comprises the eucalyptus PGEF17 gene, fragments of the gene or a plant expression vector. The invention also provides application of the gene, the plant expression vector or the host cell to promotion of vegetative growth of plant and improvement on biomass of the plant; and according to the application, the gene, or the fragments of the gene and / or the plant expression vector are transformed into the plant, or the plant is infected by using the host cell. By improving the expression of PGEF17 in the plant, the leaf number and the diameter of the plant are increased, the growth of the root is promoted, and the biomass is improved. The eucalyptus PGEF17 gene can be widely applied to improvement on the yield of forest, vegetables and industrial crop.

Description

technical field [0001] The invention relates to plant genes and applications thereof, in particular to eucalyptus PGEF17 gene and its plant expression vector, host cells and its application in promoting plant vegetative growth and increasing plant growth. Background technique [0002] Trees play an important role in air purification, windbreak and sand fixation, soil and water conservation, ecological balance and biodiversity, and provision of high-quality timber and high-yield and high-quality forest fruits. They have huge ecological benefits, social benefits and economic value. Forest trees and other woody plants are rich in gene resources and complex genetic diversity, with many genes with potential application value but not yet fully explored and effectively isolated. In recent years, with the development of molecular biology, molecular genetics, genomics and bioinformatics, and the establishment and improvement of library construction and screening technology, gene clon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/29C12N15/82C12N1/21C12N5/10C07K14/415A01H5/00
Inventor 李奕恒孙长斌许文钊
Owner 普罗米绿色能源(深圳)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap