Eucalyptus PGEF17 gene, and plant expression vector, host cell and application thereof
A plant expression vector and host cell technology, applied to plant genes and their application fields, can solve problems such as complex genetic diversity, and achieve the effects of increasing plant biomass, increasing biomass, and promoting plant vegetative growth.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0026] The present invention will be described in more detail below through specific embodiments and with reference to the accompanying drawings. It should be understood that the examples described below are only for illustration rather than limitation of the present invention.
[0027] 1. Cloning and sequence analysis of PGEF17 gene
[0028] 1. Preparation of Eucalyptus cDNA
[0029] Choose fresh eucalyptus ( Eucalyptus ) leaves, such as Eucalyptus eucalyptus species DH32-29 ( Eucalyptus urophylla × grandis cv DH32-29) leaves were quickly frozen in liquid nitrogen, and then stored in a -80°C ultra-low temperature freezer (SANYO, MDF-382E) for later use.
[0030] Total RNA was extracted from Eucalyptus leaves according to the existing Cetyltrimethyl Ammonium Bromide (CTAB) extraction method (Chang et al., Plant Molecular Biology Reporter 11(2): 113-116 (1993)). Extracted RNA was treated with RNase-free DNase (Takara, Cat# D2270A) to remove contaminating genomic DNA.
[0...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com