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Eucalyptus PGEF17 gene, and plant expression vector, host cell and application thereof

A plant expression vector and host cell technology, applied to plant genes and their application fields, can solve problems such as complex genetic diversity, and achieve the effects of increasing plant biomass, increasing biomass, and promoting plant vegetative growth.

Inactive Publication Date: 2013-01-23
普罗米绿色能源(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Forest trees and other woody plants are rich in genetic resources and complex genetic diversity, with many genes with potential application value but not yet fully explored and effectively isolated

Method used

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  • Eucalyptus PGEF17 gene, and plant expression vector, host cell and application thereof
  • Eucalyptus PGEF17 gene, and plant expression vector, host cell and application thereof
  • Eucalyptus PGEF17 gene, and plant expression vector, host cell and application thereof

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Embodiment Construction

[0026] The present invention will be described in more detail below through specific embodiments and with reference to the accompanying drawings. It should be understood that the examples described below are only for illustration rather than limitation of the present invention.

[0027] 1. Cloning and sequence analysis of PGEF17 gene

[0028] 1. Preparation of Eucalyptus cDNA

[0029] Choose fresh eucalyptus ( Eucalyptus ) leaves, such as Eucalyptus eucalyptus species DH32-29 ( Eucalyptus urophylla × grandis cv DH32-29) leaves were quickly frozen in liquid nitrogen, and then stored in a -80°C ultra-low temperature freezer (SANYO, MDF-382E) for later use.

[0030] Total RNA was extracted from Eucalyptus leaves according to the existing Cetyltrimethyl Ammonium Bromide (CTAB) extraction method (Chang et al., Plant Molecular Biology Reporter 11(2): 113-116 (1993)). Extracted RNA was treated with RNase-free DNase (Takara, Cat# D2270A) to remove contaminating genomic DNA.

[0...

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Abstract

The invention provides a eucalyptus PGEF17 gene. The complementary deoxyribonucleic acid (cDNA) sequence of the gene is characterized by having an DNA sequence which is shown as SEQIDNO:1 or encoding polypeptide which has the amino acid sequence shown as SEQIDNO:2. The invention also provides a host cell which comprises the eucalyptus PGEF17 gene, fragments of the gene or a plant expression vector. The invention also provides application of the gene, the plant expression vector or the host cell to promotion of vegetative growth of plant and improvement on biomass of the plant; and according to the application, the gene, or the fragments of the gene and / or the plant expression vector are transformed into the plant, or the plant is infected by using the host cell. By improving the expression of PGEF17 in the plant, the leaf number and the diameter of the plant are increased, the growth of the root is promoted, and the biomass is improved. The eucalyptus PGEF17 gene can be widely applied to improvement on the yield of forest, vegetables and industrial crop.

Description

technical field [0001] The invention relates to plant genes and applications thereof, in particular to eucalyptus PGEF17 gene and its plant expression vector, host cells and its application in promoting plant vegetative growth and increasing plant growth. Background technique [0002] Trees play an important role in air purification, windbreak and sand fixation, soil and water conservation, ecological balance and biodiversity, and provision of high-quality timber and high-yield and high-quality forest fruits. They have huge ecological benefits, social benefits and economic value. Forest trees and other woody plants are rich in gene resources and complex genetic diversity, with many genes with potential application value but not yet fully explored and effectively isolated. In recent years, with the development of molecular biology, molecular genetics, genomics and bioinformatics, and the establishment and improvement of library construction and screening technology, gene clon...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12N1/21C12N5/10C07K14/415A01H5/00
Inventor 李奕恒孙长斌许文钊
Owner 普罗米绿色能源(深圳)有限公司
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